US4379839A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for detecting cancer
Assignee: Columbia University
•
Filing: 1980-02-29
•
Granted: 1983-04-12
•
Expires: 2000-02-29
Abstract
Abstract The existence and status of cancers in humans can be detected by assaying for viral related proteins in plasma samples. Suitable viral related proteins include the enzyme RNA-dependent DNA polymerase (reverse transcriptase) or an extracellular tumor associated protein which is of viral origin. The aforesaid enzyme and tumor associated protein are immunologically cross-reactive with antibodies to Mason-Pfizer Monkey Virus (MPMV) and murine mammary tumor virus (MMTV) which thereby provide a convenient source of reagents for the instant method.
Inventors
- 👤 Sol Spiegelman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 21:15
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/10; C12N9/12; C12N9/1241; C12N9/1276; C12Q; C12Q1/00; C12Q1/48; C12Q1/70; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/575; G01N33/57515; G01N2474/00; G01N2474/20
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
48 words
A method for the detection of breast cancer in a human subject, which method comprises immunologically assaying a sample from said subject for breast cancer specific viral related protein, the assay utilizing the cross-reactivity of the protein with antibodies to Mason-Pfizer Monkey Virus or murine mammary tumor virus.
Claim 2
Independent Claim
48 words
A method for the detection of breast cancer in a human subject, which method comprises immunologically assaying a sample from said subject for breast cancer specific viral related protein, the assay utilizing the cross-reactivity of the protein with antibodies to Mason-Pfizer Monkey Virus or murine mammary tumor virus.
Claim 3
Dependent Claim
30 words
The method of claim 1 wherein plasma samples are taken from said subject before and after initiation of therapy and said assay serves to monitor the effectiveness of said therapy.
Claim 4
Dependent Claim
27 words
The method of claim 1 wherein said breast cancer specific viral related protein is human breast cancer DNA polymerase and said sample is a blood plasma sample.
Claim 5
Dependent Claim
13 words
The method of claim 3 wherein said DNA polymerase is assayed by radioimmunoassay.
Claim 6
Dependent Claim
23 words
The method of claim 4 wherein said radioimmunoassay utilizes human breast cancer DNA polymerase specific antibody and 125 I-human breast cancer DNA polymerase.
Claim 7
Dependent Claim
40 words
The method of claim 4 wherein said radioimmunoassay utilizes Mason-Pfizer Monkey Virus or murine mammary tumor virus specific antibody, which are cross-reactive with human breast cancer DNA polymerase, and 125 I-Mason-Pfizer Monkey Virus or 125 I-murine mammary tumor virus, respectively.
Claim 8
Dependent Claim
27 words
The method of claim 1 wherein said breast cancer specific viral related protein is viral origin tumor associated protein and said sample is a blood plasma sample.
Claim 9
Dependent Claim
16 words
The method of claim 7 wherein said viral origin tumor associated protein is assayed by radioimmunoassay.
Claim 10
Dependent Claim
23 words
The method of claim 8 wherein said radioimmunoassay utilizes viral origin tumor associated protein specific antibody and 125 I-viral origin tumor associated protein.
Claim 11
Dependent Claim
39 words
The method of claim 8 wherein said radioimmunoassay utilizes Mason-Pfizer Monkey Virus or murine mammary tumor virus specific antibody, wich are cross-reactive with said tumor associated protein, and 125 I-Mason-Pfizer Monkey Virus or 125 I-murine mammary tumor virus, respectively.
Claim 12
Independent Claim
98 words
A method for the immunohistological detection of breast cancer which method comprises treating a human breast tissue section with a first antibody against a virus selected from Mason-Pfizer Monkey Virus and murine mammary tumor virus, thereafter treating said tissue section with a second antibody directed against the first antibody, which second antibody is conjugated to peroxidase enzyme and then treating the resulting tissue section with a substrate of said enzyme which forms a colored precipitate on contact with said enzyme whereby positive staining of said tissue section is indicative of the presence of malignant cells in said tissue.
Claim 13
Independent Claim
98 words
A method for the immunohistological detection of breast cancer which method comprises treating a human breast tissue section with a first antibody against a virus selected from Mason-Pfizer Monkey Virus and murine mammary tumor virus, thereafter treating said tissue section with a second antibody directed against the first antibody, which second antibody is conjugated to peroxidase enzyme and then treating the resulting tissue section with a substrate of said enzyme which forms a colored precipitate on contact with said enzyme whereby positive staining of said tissue section is indicative of the presence of malignant cells in said tissue.
Claim 14
Dependent Claim
15 words
The method of claim 11 wherein said first antibody is murine mammary tumor virus antibody.
Claim 15
Dependent Claim
13 words
The method of claim 12 wherein said second antibody is goat anti-rabbit IgG.
Claim 16
Dependent Claim
13 words
The method of claim 11 wherein said peroxidase enzyme is horseradish peroxidase enzyme.
References Cited (Backward Citations)
US3867363A
Cited
US3755086A
Cited
US3816263A
Cited
CA977681A
Cited
US3798131A
Cited
US3852415A
Cited
US3859430A
Cited
US4002532A
Cited
US3999944A
Cited
US4043757A
Cited
US4016043A
Cited
US3988115A
Cited
US4146603A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-04-11 | 1986-10-11 | 1987-04-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-04-11 | 1990-10-11 | 1991-04-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-04-11 | 1994-10-11 | 1995-04-12 | $7700.00 | Window Closed / Lapsed |