US3988115A
Expired / Lapsed
US
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Diagnostic method for determining pathological condition by antigen-combining capacity of lymphocytes
Assignee: Individual
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Filing: 1975-09-18
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Granted: 1976-10-26
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Expires: 1995-09-18
Abstract
Abstract A method for determining in a patient the presence or absence of a pathological condition producing a specific immune response. The antigen associated with the pathological condition is labeled and added to a sample of the patient's lymphocytes and to a control sample. Comparison of the antigen-binding capacity of the patient's lymphocytes to that of the control sample is related to the presence or absence of the pathological condition.
Inventors
- 👤 Farrokh Z. Modabber
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 23:52
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/554; G01N33/5005; G01N33/5091; G01N33/569; G01N33/56966; G01N33/56972; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/871; Y10S435/911; Y10S436/00; Y10S436/80; Y10S436/811
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
208 words
A method for determining in a patient the presence or absence of a pathological condition which produces at least one specific immune response which comprises: a. extracting from the patient a sample of blood, b. providing a control sample containing particles of predetermined binding activity with respect to an antigen associated with said pathological condition, c. adding to at least the lymphocyte fraction of said patient's blood and of said control sample an excess of a detection agent comprising an antigen for said pathological condition linked to a marker material whereby a portion of said detection agent attaches to a portion of the lymphocytes in said lymphocyte fraction, d. eliminating from each of said lymphocyte fractions any unattached detection agent, e. determining the amount of attached detection agent in each of said lymphocyte fractions through determination of the amount of said marker material therein and calculating the amount of attached detection agent in each of said lymphocyte fractions per designated number of white blood cells, and f. comparing the amount of said marker material per designated number of white blood cells in said blood of said patient with the amount of said marker material per designated number of white blood cells in the blood of said control sample.
Claim 2
Independent Claim
208 words
A method for determining in a patient the presence or absence of a pathological condition which produces at least one specific immune response which comprises: a. extracting from the patient a sample of blood, b. providing a control sample containing particles of predetermined binding activity with respect to an antigen associated with said pathological condition, c. adding to at least the lymphocyte fraction of said patient's blood and of said control sample an excess of a detection agent comprising an antigen for said pathological condition linked to a marker material whereby a portion of said detection agent attaches to a portion of the lymphocytes in said lymphocyte fraction, d. eliminating from each of said lymphocyte fractions any unattached detection agent, e. determining the amount of attached detection agent in each of said lymphocyte fractions through determination of the amount of said marker material therein and calculating the amount of attached detection agent in each of said lymphocyte fractions per designated number of white blood cells, and f. comparing the amount of said marker material per designated number of white blood cells in said blood of said patient with the amount of said marker material per designated number of white blood cells in the blood of said control sample.
Claim 3
Dependent Claim
12 words
The method of claim 1 wherein said marker material is an enzyme.
Claim 4
Dependent Claim
10 words
The method of claim 2 wherein said enzyme is beta-galactosidase.
Claim 5
Dependent Claim
19 words
The method of claim 1 wherein said unattached detection agent is eliminated by the washing of said lymphocyte fraction.
Claim 6
Dependent Claim
24 words
The method of claim 1 wherein said pathological condition in an amebic infection and said antigen is derived from a culture of Entamoeba histolytica.
Claim 7
Dependent Claim
22 words
The method of claim 1 wherein said pathological condition is gonorrhea and said antigen is derived from a culture of Neisseria gonorrheae.
Claim 8
Dependent Claim
22 words
The method of claim 1 wherein said pathological condition is histoplasmosis and said antigen is derived from a culture of Histoplasma capsulatum.
Claim 9
Dependent Claim
13 words
The method of claim 1 wherein said pathological condition is a virus infection.
Claim 10
Dependent Claim
13 words
The method of claim 1 wherein said pathological condition is an allergic sensitivity.
Claim 11
Dependent Claim
24 words
The method of claim 1 wherein said control sample is a sample of blood from at least one individual free of said pathological condition.
Claim 12
Independent Claim
70 words
A method for determining in a patient the presence or absence of a pathological condition which produces lymphocyte proliferation in the blood which comprises extracting from said patient a sample of blood, determining in said sample the proportion of lymphocytes containing receptors specific for an antigen for said pathological condition and comparing said proportion to the proportion of such lymphocytes in the blood of individual free of said pathological condition.
Claim 13
Independent Claim
70 words
A method for determining in a patient the presence or absence of a pathological condition which produces lymphocyte proliferation in the blood which comprises extracting from said patient a sample of blood, determining in said sample the proportion of lymphocytes containing receptors specific for an antigen for said pathological condition and comparing said proportion to the proportion of such lymphocytes in the blood of individual free of said pathological condition.
Claim 14
Dependent Claim
39 words
The method of claim 11 wherein the determination of said lymphocytes sensitive to an antigen for said pathological condition comprises attaching said lymphocytes to a detection agent comprising an antigen for said pathological condition linked to a marker material.
Claim 15
Dependent Claim
12 words
The method of claim 12 wherein said marker material is an enzyme.
Claim 16
Dependent Claim
10 words
The method of claim 13 wherein said enzyme is beta-galactosidase.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-10-26 | 1980-04-26 | 1980-10-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-10-26 | 1984-04-26 | 1984-10-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-10-26 | 1988-04-26 | 1988-10-26 | $7700.00 | Window Closed / Lapsed |