US3755086A
Expired / Lapsed
US
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Diagnostic method utilizing synthetic deoxyrilionucleotide oligomer template
Assignee: F Hoffmann La Roche AG
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Filing: 1971-02-09
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Granted: 1973-08-28
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Expires: 1991-02-09
Abstract
Abstract A diagnostic method for the detection of virus-related neoplastic disease states is described. This method involves employing synthetic nucleotide oligomers hybridized with RNA-type polymers as a template for assaying RNA-dependent DNA polymerase activity. RNA-dependent DNA polymerase activity has been found to be specifically characteristic of several neoplastic disease states including human leukemia. In a preferred embodiment the instant method employs synthetic thymidylic acid oligomers (d-pT) hybridized with polymeric ribonucleotide rA.
Inventors
- 👤 E Heimer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 04:40
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/48; C12Q1/68
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
15 words
The method of claim 1 wherein said synthetic nucleotide oligomer is a linear thymidine polynucleotide.
Claim 2
Dependent Claim
12 words
The method of claim 2 wherein said synthetic nucleotide oligomer is d-pT6.
Claim 3
Dependent Claim
12 words
The method of claim 2 wherein said synthetic nucleotide oligomer is d-pT8.
Claim 4
Dependent Claim
12 words
The method of claim 2 wherein said synthetic nucleotide oligomer is d-pT9.
Claim 5
Dependent Claim
12 words
The method of claim 2 wherein said synthetic nucleotide oligomer is d-pT3.
Claim 6
Dependent Claim
11 words
The method of claim 1 wherein said RNA-type polymer is rA.
Claim 7
Dependent Claim
14 words
The method of claim 1 wherein said sample is obtained from human leukemia cells.
Claim 8
Dependent Claim
28 words
The method of claim 1 wherein said sample is a mammalian cellular extract obtained from whole blood which has been purified by running it through a glycerol gradient.
Claim 9
Dependent Claim
73 words
The method of claim 9 wherein said reaction mixture comprises 100-200 Mu g. of purified sample, 1-5 Mu g. of said template; and is 4 X 10 2 M with respect to K and 6 X 10 3 M with respect to Mg ; and contains 800 m Mu moles/ml. of two deoxyribonucleotides complementary to said template, at least one of which is isotopically labeled having a specific activity of about 500 cpm/pmole.
Claim 10
Dependent Claim
83 words
The method of claim 1 wherein said sample is a purified plasma obtained from whole blood purified by treatment with Kieselguhr and said reaction mixture comprises 25 Mu g. of said purified sample; is 0.006 M in Mg is 0.04 M in K , is 0.008 M in a first complementary nucleoside triphosphate and is 0.00016 M in an isotopically labeled second complementary nucleoside triphosphate having a specific activity of about 100 cpm/pmole, and contains about 1 Mu g. of said synthetic template.
References Cited (Backward Citations)
US3597318A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-08-27 | 1977-02-26 | 1977-08-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-08-27 | 1981-02-26 | 1981-08-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-08-27 | 1985-02-26 | 1985-08-28 | $7700.00 | Window Closed / Lapsed |