US3852415A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Compositions for use in radioimmunoassay as a substitute for blood plasma extract in determination of carcinoembryonic antigen
Assignee: F Hoffmann La Roche AG
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Filing: 1972-10-13
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Granted: 1974-12-03
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Expires: 1992-10-13
Abstract
Abstract A composition suitable for use as a substitute for blood plasma extract in producing antiserum titration curves and standard antigen inhibition curves for the determination of carcinoembryonic antigen in human plasma is disclosed.
Inventors
- 👤 J Vandervoorde
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 21:03
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/96; Y; Y10; Y10S; Y10S435/00; Y10S435/967; Y10S436/00; Y10S436/811; Y10S436/813; Y10T; Y10T436/00; Y10T436/10; Y10T436/106664; Y10T436/107497; Y10T436/108331
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
95 words
A DILUENT COMPOSITION SUITABLE FOR FORMING AN ANTIBODY TO CEA TITRATION CURVE OR A CEA STANDARD INHIBITION CURVE SUBSTANTIALLY IDENTICAL TO THAT RESULTING WHEN BLOOD PLASMA IS USED AS THE DILUENT, SAID COMPOSITION HAVING A PH 6.45-6.55 AND COMPRISING PER LITER, AN AMOUNT OF A SALT OF AN ORGANIC CARBOXYLIC ACID WITH AN ALKALI METAL WHICH IS EQUIVALENT TO 1.3 GRAMS OF DISODIUM ETHYLENEDIAMINE TETRAACETIC AND DIHYDRATE, 0.17 GRAMS OF AN ANTIMICROBIAL PRESERVATIVE, 70 UL. OF A 30 PERCENT W/W AQUEOUS SOLUTION OF BOVINE SERUM ALBUMIN, SODIUM HYDROXIDE, AND SUFFICIENT WATER TO MAKE 1 LITER.
Claim 2
Dependent Claim
95 words
A DILUENT COMPOSITION SUITABLE FOR FORMING AN ANTIBODY TO CEA TITRATION CURVE OR A CEA STANDARD INHIBITION CURVE SUBSTANTIALLY IDENTICAL TO THAT RESULTING WHEN BLOOD PLASMA IS USED AS THE DILUENT, SAID COMPOSITION HAVING A PH 6.45-6.55 AND COMPRISING PER LITER, AN AMOUNT OF A SALT OF AN ORGANIC CARBOXYLIC ACID WITH AN ALKALI METAL WHICH IS EQUIVALENT TO 1.3 GRAMS OF DISODIUM ETHYLENEDIAMINE TETRAACETIC AND DIHYDRATE, 0.17 GRAMS OF AN ANTIMICROBIAL PRESERVATIVE, 70 UL. OF A 30 PERCENT W/W AQUEOUS SOLUTION OF BOVINE SERUM ALBUMIN, SODIUM HYDROXIDE, AND SUFFICIENT WATER TO MAKE 1 LITER.
Claim 3
Dependent Claim
78 words
A diluent composition suitable for forming an antibody to CEA titration curve or a CEA standard inhibition curve substantially identical to that resulting when blood plasma is used as the diluent, said composition having a pH 6.45-6.55 and comprising per liter; 1.3 grams of disodium ethylenediamine tetraacetic acid dihydrate, 0.17 grams of an antimicrobial preservative, 70 Mu l. of a 30 percent w/w aqueous solution of bovine serum albumin, sodium hydroxide, and sufficient water to make 1 liter.
Claim 4
Dependent Claim
78 words
A diluent composition suitable for forming an antibody to CEA titration curve or a CEA standard inhibition curve substantially identical to that resulting when blood plasma is used as the diluent, said composition having a pH 6.45-6.55 and comprising per liter; 1.3 grams of disodium ethylenediamine tetraacetic acid dihydrate, 0.17 grams of an antimicrobial preservative, 70 Mu l. of a 30 percent w/w aqueous solution of bovine serum albumin, sodium hydroxide, and sufficient water to make 1 liter.
Claim 5
Dependent Claim
11 words
The composition of claim 2 wherein the preservative is sodium azide.
Claim 6
Dependent Claim
71 words
A diluent composition suitable for forming an antibody to CEA titration curve or a CEA standard inhibition curve substantially identical to that resulting when blood plasma is used as the diluent, said composition having a pH 6.25 and comprising per liter; 13.0 grams of disodium EDTA dihydrate, 1.7 grams of an antimicrobial preservative, 700 Mu l. of 30 percent w/w aqueous BSA, sodium hydroxide, and sufficient water to make 1 liter.
Claim 7
Dependent Claim
71 words
A diluent composition suitable for forming an antibody to CEA titration curve or a CEA standard inhibition curve substantially identical to that resulting when blood plasma is used as the diluent, said composition having a pH 6.25 and comprising per liter; 13.0 grams of disodium EDTA dihydrate, 1.7 grams of an antimicrobial preservative, 700 Mu l. of 30 percent w/w aqueous BSA, sodium hydroxide, and sufficient water to make 1 liter.
Claim 8
Dependent Claim
11 words
The composition of claim 4 wherein the preservative is sodium azide.
Claim 9
Dependent Claim
78 words
In a method of making an antibody to CEA titration curve by forming graded amounts of antiserum to CEA by dilution with a buffer, incubating in a diluent, adding radiolabelled CEA, incubating, adding zirconyl phosphate gel, separating the resulting precipitated gel bound with radiolabelled material, assaying it for radioactivity and recording the results on a graph as counts per minute vs. Mu liters of antiserum, the improvement which comprises utilizing as the diluent, the composition of claim 1.
Claim 10
Dependent Claim
75 words
In a method of making a CEA standard inhibition curve by adding antiserum to CEA to graded amounts of CEA in a diluent, incubating the mixture, adding radiolabelled CEA, incubating, adding zirconyl phosphate gel, recovering the resulting gel precipitate bound with radiolabelled material, assaying it for radioactivity and recording the results on a graph as counts per minute vs. nanograms of CEA, the improvement which comprises utilizing as the diluent, the composition of claim 1.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-12-02 | 1978-06-03 | 1978-12-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-12-02 | 1982-06-03 | 1982-12-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-12-02 | 1986-06-03 | 1986-12-03 | $7700.00 | Window Closed / Lapsed |