US4366242A
Expired / Lapsed
US
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Method and agent for the immunological determination of enzymes
Assignee: Merck Patent GmbH
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Filing: 1980-12-29
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Granted: 1982-12-28
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Expires: 2000-12-29
Abstract
Abstract A process for the quantitative immunological determination of an enzyme in a liquid, comprises incubating anti-enzyme antibodies which are adsorbed on a water-insoluble carrier, with the sample solution containing the enzyme, whereby the enzyme bonds to the antibodies but retains a reproducible proportion of its activity in its complex with the antibodies; removing the sample solution; washing the antibody carrier, which is charged with enzyme; and determining the activity of the enzyme bonded to the antibodies.
Inventors
- 👤 Siegfried Neumann
- 👤 Norbert Hennrich
- 👤 Hans-Dieter Orth
- 👤 Gerhard Pfleiderer
- 👤 Evangelista Jockers-Wretou
- 👤 Hans Pauly
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 02:24
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/573; Y; Y10; Y10S; Y10S435/00; Y10S435/975
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
83 words
A process for the quantitative immunological determination of an isoenzyme of the acid or alkaline phosphatase in body fluid, comprising incubating anti-isoenzyme antibodies which are absorbed on a water-insoluble carrier, with the sample solution containing the isoenzyme, whereby the isoenzyme bonds to the antibodies but retains a measurable reproducible proportion of its activity in its complex with the antibodies; removing the sample solution; washing the antibody carrier which is charged with isoenzyme; and determining the activity of the isoenzyme bonded to the antibodies.
Claim 2
Dependent Claim
83 words
A process for the quantitative immunological determination of an isoenzyme of the acid or alkaline phosphatase in body fluid, comprising incubating anti-isoenzyme antibodies which are absorbed on a water-insoluble carrier, with the sample solution containing the isoenzyme, whereby the isoenzyme bonds to the antibodies but retains a measurable reproducible proportion of its activity in its complex with the antibodies; removing the sample solution; washing the antibody carrier which is charged with isoenzyme; and determining the activity of the isoenzyme bonded to the antibodies.
Claim 3
Dependent Claim
18 words
A process of claim 1 wherein the isoenzyme is the prostate isoenzyme of acid phosphatase in body fluids.
Claim 4
Dependent Claim
13 words
A process of claim 1 wherein the water-insoluble carrier is a plastic vessel.
Claim 5
Dependent Claim
16 words
A process of claim 3 wherein the water-insoluble carrier is a polystyrene or polypropylene test tube.
Claim 6
Dependent Claim
66 words
A test system for immunochemically determining an isoenzyme of either acid or alkaline phosphatase comprising, an antibody bonded by adsorption to a water insoluble carrier wherein said antibody is specific for an isoenzyme of either acid phosphatase or alkaline phosphatase and is capable of binding to the isoenzyme in a manner such that the isoenzyme retains an analytically significant measurable and reproducible portion of its activity.
Claim 7
Dependent Claim
66 words
A test system for immunochemically determining an isoenzyme of either acid or alkaline phosphatase comprising, an antibody bonded by adsorption to a water insoluble carrier wherein said antibody is specific for an isoenzyme of either acid phosphatase or alkaline phosphatase and is capable of binding to the isoenzyme in a manner such that the isoenzyme retains an analytically significant measurable and reproducible portion of its activity.
Claim 8
Dependent Claim
13 words
A water-insoluble carrier of claim 5 wherein the carrier is a plastic vessel.
Claim 9
Dependent Claim
16 words
A water-insoluble carrier of claim 9 wherein the carrier is a polystyrene or polypropylene test tube.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-12-27 | 1986-06-28 | 1986-12-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-12-27 | 1990-06-28 | 1990-12-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-12-27 | 1994-06-28 | 1994-12-28 | $7700.00 | Window Closed / Lapsed |