US3966898A
Expired / Lapsed
US
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Method and reagent for determining immunologic materials
Assignee: Phadia AB
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Filing: 1974-06-19
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Granted: 1976-06-29
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Expires: 1994-06-19
Abstract
Abstract A method of qualitatively or quantitatively indicating immunoglobulin whose Fab-portion is bound to or is caused to bind to an antigen or a hapten or indicating the antigen or hapten which is bound to or caused to bind to the Fab-portion of the immunoglobulin, the immunoglobulin being labelled -- before or after binding to the antigen or hapten -- with one or more analytically indicatable atoms or groups by contact with a polypeptide labelled with the analytically indicatable atom or group or atoms or groups, wherein the immunoglobulin belongs to the IgG-class and the polypeptide is one which is obtained from microorganisms and which is able to bind itself to the Fc-portion of said immunoglobulin belonging to the IgG-class. The method utilizes an auxiliary agent for labelling immunoglobulin and a reagent for quantitatively or qualitatively indicating antigens or haptens.
Inventors
- 👤 John A. Sjoquist
- 👤 Arne V. Sjodin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:10
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/563; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/68; A61K47/6801; A61K47/6803; A61K47/6811; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/828; Y10S530/00; Y10S530/806; Y10S530/862
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
95 words
In the method of qualitatively or quantitatively indicating an immunoglobulin belonging to the IgG-class, the Fab-portion of which immunoglobulin is bound to an antigen or hapten, the immunoglobulin being labelled, before or after binding to the antigen or hapten, with one or more analytically indicatable atoms or groups by contacting said immunoglobulin with a polypeptide labelled with said analytically indicatable atoms or groups, the improvement which comprises using as the polypeptide, a polypeptide which is obtained from Staphylococci and which is able to bind itself to the Fc-portion of said immunoglobulin belonging to the IgG-class.
Claim 2
Dependent Claim
95 words
In the method of qualitatively or quantitatively indicating an immunoglobulin belonging to the IgG-class, the Fab-portion of which immunoglobulin is bound to an antigen or hapten, the immunoglobulin being labelled, before or after binding to the antigen or hapten, with one or more analytically indicatable atoms or groups by contacting said immunoglobulin with a polypeptide labelled with said analytically indicatable atoms or groups, the improvement which comprises using as the polypeptide, a polypeptide which is obtained from Staphylococci and which is able to bind itself to the Fc-portion of said immunoglobulin belonging to the IgG-class.
Claim 3
Dependent Claim
38 words
A method as set forth in claim 1, wherein the indication of the immunoglobulin belonging to the IgG-class is used for the indication of an antigen or a hapten which is bound to the Fab-portion of said immunoglobulin.
Claim 4
Dependent Claim
96 words
A reagent for quantitatively or qualitatively indicating antigens or haptens in free or bound form, which reagent comprises a reaction product between (a) an immunoglobulin belonging to the IgG-class and having a Fab-portion which has an antibody activity against an antigen or a hapten and (b) a polypeptide which is obtained from Staphylococci and subsequently labelled and which is able to bind to the Fc-portion of said immunoglobulin belonging to the IgG-class, the polypeptide being bound to the Fc-portion of the immunoglobulin leaving the antibody active Fab-portion of the immunoglobulin free to bind antigen or hapten.
Claim 5
Dependent Claim
96 words
A reagent for quantitatively or qualitatively indicating antigens or haptens in free or bound form, which reagent comprises a reaction product between (a) an immunoglobulin belonging to the IgG-class and having a Fab-portion which has an antibody activity against an antigen or a hapten and (b) a polypeptide which is obtained from Staphylococci and subsequently labelled and which is able to bind to the Fc-portion of said immunoglobulin belonging to the IgG-class, the polypeptide being bound to the Fc-portion of the immunoglobulin leaving the antibody active Fab-portion of the immunoglobulin free to bind antigen or hapten.
References Cited (Backward Citations)
US3859430A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-06-29 | 1979-12-29 | 1980-06-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-06-29 | 1983-12-29 | 1984-06-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-06-29 | 1987-12-29 | 1988-06-29 | $7700.00 | Window Closed / Lapsed |