US4298592A
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Double antibody separation method
Assignee: Takeda GmbH
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Filing: 1979-04-05
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Granted: 1981-11-03
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Expires: 1999-04-05
Abstract
Abstract A double antibody radioimmunoassay method for determining antigens in a sample wherein polyethylene glycol is provided in a reaction medium to accelerate an immunoprecipitation reaction between water-soluble first antibodies bound to antigen and water-soluble second antibodies which are not specific to said antigens but which are specific to said first antibodies. A double antibody, multi-component radioimmunoassay test kit is also provided. The kit contains radiolabeled antigen reagent, first water-soluble antibody reagent and second water-soluble antibody reagent, as well as a polyethylene glycol reagent, which may optionally be combined with the second antibody reagent, in an amount sufficient to accelerate an immunoprecipitation reaction, between said first and second reagents.
Inventors
- 👤 Wayne H. T. Lin
- 👤 James J. Grib
- 👤 Larry D. Mosier
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 02:43
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/537; G01N33/539; G01N33/5306; Y; Y10; Y10S; Y10S435/00; Y10S435/975; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/808
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
89 words
A double antibody radioimmunoassay method for determining antigens in a sample, comprising providing polyethylene glycol having a molecular weight of from about 200 to about 10,000 in a reaction medium containing labeled antigen, said polyethylene glycol being present in said reaction medium in an amount sufficient to accelerate an immunoprecipitation reaction between water-soluble first antibodies in a first antibody reagent bound to antigens and water-soluble antibodies in a second antibody reagent which are not specific to said antigens to be assayed but which are specific to said first antibodies.
Claim 2
Dependent Claim
89 words
A double antibody radioimmunoassay method for determining antigens in a sample, comprising providing polyethylene glycol having a molecular weight of from about 200 to about 10,000 in a reaction medium containing labeled antigen, said polyethylene glycol being present in said reaction medium in an amount sufficient to accelerate an immunoprecipitation reaction between water-soluble first antibodies in a first antibody reagent bound to antigens and water-soluble antibodies in a second antibody reagent which are not specific to said antigens to be assayed but which are specific to said first antibodies.
Claim 3
Dependent Claim
20 words
The method of claim 1 wherein said polyethylene glycol has a molecular weight of from bout 4,000 to about 7,500.
Claim 4
Dependent Claim
28 words
The method of claim 1 wherein said sample is selected from blood serum and blood plasma and said antigen is selected from prostatic acid phosphatase and creatine phosphokinase.
Claim 5
Dependent Claim
22 words
The method of claim 1 wherein said first antibody is specific to an antigen selected from prostatic acid phosphatase and creatine phosphokinase.
Claim 6
Dependent Claim
93 words
A package for a double antibody radioimmunoanalysis of a sample for an antigen which comprises: a radiolabeled antigen reagent; a first, water-soluble antibody reagent specific to said antigen: a second, water-soluble antibody reagent which is not specific to the antigen but which is specific to the first antibody, said second antibody reagent containing polyethylene glycol having a molecular weight of from about 2,000 to 10,000 in an amount sufficient to accelerate separation of the reaction product of second, water-soluble antibody reagent and antigen bound to said first antibody reagent in a reaction medium.
Claim 7
Dependent Claim
93 words
A package for a double antibody radioimmunoanalysis of a sample for an antigen which comprises: a radiolabeled antigen reagent; a first, water-soluble antibody reagent specific to said antigen: a second, water-soluble antibody reagent which is not specific to the antigen but which is specific to the first antibody, said second antibody reagent containing polyethylene glycol having a molecular weight of from about 2,000 to 10,000 in an amount sufficient to accelerate separation of the reaction product of second, water-soluble antibody reagent and antigen bound to said first antibody reagent in a reaction medium.
Claim 8
Dependent Claim
95 words
A package for a double antibody radioimmunoanalysis of a sample for an antigen which comprises: a radiolabeled antigen reagent; a first water-soluble antibody reagent specific to said antigen; a second, water-soluble antibody reagent which is not specific to the antigen but which is specific to the first antibody, and a polyethylene glycol reagent containing polyethylene glycol having a molecular weight of from about 2,000 to 10,000 in an amount sufficient to accelerate separation of the reaction product of second, water-soluble antibody reagent and radiolabeled antigen bound to said first antibody reagent in a reaction medium.
Claim 9
Dependent Claim
95 words
A package for a double antibody radioimmunoanalysis of a sample for an antigen which comprises: a radiolabeled antigen reagent; a first water-soluble antibody reagent specific to said antigen; a second, water-soluble antibody reagent which is not specific to the antigen but which is specific to the first antibody, and a polyethylene glycol reagent containing polyethylene glycol having a molecular weight of from about 2,000 to 10,000 in an amount sufficient to accelerate separation of the reaction product of second, water-soluble antibody reagent and radiolabeled antigen bound to said first antibody reagent in a reaction medium.
Claim 10
Dependent Claim
20 words
The package of claim 5 or 6, which also includes pure antigen reagent for preparation of standard and control samples.
Claim 11
Dependent Claim
24 words
The package of claim 5 or 6, wherein sad reagents are provided in an aqueous solution having a pH of about 4 to 10.
Claim 12
Dependent Claim
19 words
The package of claim 5 or 6, wherein said antigen is selected from prostatic acid phosphatase; and creatine phosphokinase.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-11-02 | 1985-05-04 | 1985-11-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-11-02 | 1989-05-04 | 1989-11-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-11-02 | 1993-05-04 | 1993-11-03 | $7700.00 | Window Closed / Lapsed |