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US3936355A
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Microorganism growth media and the stabilization thereof
Assignee: University of California, San Diego
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Filing: 1973-11-12
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Granted: 1976-02-03
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Expires: 1993-11-12
Abstract
Abstract Media for supporting the growth of certain types of microorganisims, especially Neisseria gonorrhoeae and Neisseria meningitidis, are described. These media, when supplemented with polyvinylpyrrolidone (PVP), albumin and penicillin induce microorganism transformation to the wall-defective or "L-form." The PVP is necessarily "detoxified" for use in the L-form inducing media.
Inventors
- 👤 John W. Lawson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 18:31
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/12; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/871
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
48 words
A medium for the induction of the wall defective forms of cocci, including the genus neisseria, comprising a standard virology tissue culture medium known as Medium 199, and added amounts of cysteine, glycerophosphate, alkali metal chloride salts, ammonium chloride salt, magnesium sulfate, glucose, detoxified polyvinylpyrrolidone albumin, and penicillin.
Claim 2
Independent Claim
48 words
A medium for the induction of the wall defective forms of cocci, including the genus neisseria, comprising a standard virology tissue culture medium known as Medium 199, and added amounts of cysteine, glycerophosphate, alkali metal chloride salts, ammonium chloride salt, magnesium sulfate, glucose, detoxified polyvinylpyrrolidone albumin, and penicillin.
Claim 3
Dependent Claim
8 words
The medium of claim 1 solidified with agarose.
Claim 4
Dependent Claim
85 words
The medium of claim 1 wherein the components are present in the amounts of about 50 ml/l of the standard virology tissue culture, and added amounts of about 0.012 g/l of cysteine, about 2.5 g/l of glycerophosphate, about 0.09 g/l of KCl, about 6 g/l of NaCl, about 1.25 g/l of NH 4 Cl, about 0.6 g/l of MgSO 4 .7H 2 O, about 5 g/l of glucose, about 2% by weight albumin, about 8% by weight detoxified polyvinylpyrrolidone, and about 20 units/ml of penicillin.
Claim 5
Dependent Claim
8 words
The medium of claim 3 solidified with agarose.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-02-02 | 1979-08-04 | 1980-02-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-02-02 | 1983-08-04 | 1984-02-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-02-02 | 1987-08-04 | 1988-02-03 | $7700.00 | Window Closed / Lapsed |