US3669846A
Expired / Lapsed
US
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Process for obtaining and preserving stable bacterial variants
Assignee: Laboratoires Albert Rolland SA
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Filing: 1970-03-23
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Granted: 1972-06-13
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Expires: 1990-03-23
Abstract
Abstract The invention provides a process for obtaining and preserving stable bacterial variants arising from pathogenic or nonpathogenic bacteria, the said process comprising subjecting the bacteria, in an acellular medium, at the moment of scissiparity, to the action of an inducing agent, especially plasmolysis by osmotic shock, so as to do away with the cell wall, and culturing the variants obtained on an osmotically equilibrated acellular nutrient medium. The bacterial variants so obtained can be used for the preparation of vaccines and of reagents for serodiagnostics and the identification of germs of atypical illnesses.
Inventors
- 👤 Yvonne Thuillier
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 17:41
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/01; C12N1/00; C12N1/20; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/873
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
14 words
Process according to claim 1, in which the agent is plasmolysis by osmotic shock.
Claim 2
Dependent Claim
26 words
Process according to claim 2, in which the plasmolysis is effected in an acellular medium under an osmotic pressure greater than or equal to 60 atmospheres.
Claim 3
Dependent Claim
13 words
Process according to claim 3 in which the pressure is about 80 atmospheres.
Claim 4
Dependent Claim
22 words
Process according to claim 3, in which the bacteria subjected to the plasmolysis are the Enterobacteriaceae, Microccocaceae, Pseudomonadaceae, Parvobacteriaceae, Actinomycetes or Vibrionaceae.
Claim 5
Dependent Claim
15 words
Process according to claim 5, in which the bacteria subjected to plasmolysis is Proteus rettgeri.
Claim 6
Dependent Claim
16 words
Process according to claim 3 in which the acellular plasmolysis medium contains sucrose and inorganic salts.
Claim 7
Dependent Claim
18 words
Process according to claim 1, in which the acellular culture and transplantation medium contains sucrose and inorganic salts.
Claim 8
Dependent Claim
217 words
Process according to claim 1 in which a culture of Proteus rettgeri, 24 hours old, in an aqueous culture medium containing, in grams per thousand trams of culture medium, 20 g. of pancreatic peptone, 0.10 g. of nucleic acids, 0.01 g. of adenosine diphosphate and 2 g. of glucose, is introduced into an aqueous hypertonic solution of pH between 7.5 and 8.4 having an osmotic pressure of 80 atmospheres and containing, in grams per thousand grams of solution, 102 g. of 0.3 M sucrose, 4 g. of CaCl2, 40 g. of NaCl, 1 g. of NaHCO3, 14. g. of MgCl2 . 6H2O, 18 g. of Na2SO4 and 0.6 g. of KCl, at the rate of 0.1 ml. of culture per 10 ml. of hypertonic solution so as to undergo plasmolysis for 12 to 24, and the variants obtained are transplanted and cultured at 37* C in an aqueous hypertonic nutrient medium containing, in grams per thousand grams of the said medium, 20 g. of pancreatic peptone, 0.10 g. of nucleic acids, 0.01 g. of adenosine diphosphate, 2 g. glucose, 102 g. of 0.3 M sucrose, 18 g. of NaCl, 2 g. of NaHCO3, 0.10 g. of MgCl2 . 6H2O, 0.40 g. of MgSO4 . 7H2O and 0.40 g. of KCl, and transplanted every 4 to 6 weeks.
Claim 9
Dependent Claim
27 words
Process according to claim 9, in which the transplantation of the variants obtained is effected during the spheroidal phase of the evolutionary cycle of the said variants.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-06-13 | 1975-12-13 | 1976-06-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-06-13 | 1979-12-13 | 1980-06-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-06-13 | 1983-12-13 | 1984-06-13 | $7700.00 | Window Closed / Lapsed |