US3816261A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Culture medium for lertospira organisms
Assignee: Johnson and Johnson
•
Filing: 1971-04-01
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Granted: 1974-06-11
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Expires: 1991-04-01
Abstract
Abstract A chemically defined, protein-free culture medium embodying an anion-exchange resin and a water-soluble lipid source provides means for producing high yields of Leptospira organisms.
Inventors
- 👤 H Torney
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 10:42
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/02; A61K39/0225; C; C12; C12N; C12N1/00; C12N1/20; C12N1/38; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
14 words
A composition in accordance with claim 1 wherein the lipid source is a polysorbate.
Claim 2
Dependent Claim
16 words
The composition of claim 2 wherein the lipid source is the polyoxyethylene derivative of sorbitan monostearate.
Claim 3
Dependent Claim
16 words
The composition of claim 1 wherein the anion-exchange resin is a weakly basic anion exchange resin.
Claim 4
Independent Claim
98 words
A method for culturing Leptospira organisms which comprises inoculating said organisms into a sterile, protein-free culture medium consisting essentially of a buffered aqueous solution of a water-soluble lipid source selected from the group consisting of sodium oleate, sodium stearate and polysorbates thereof, glucose and essential amino acids, mineral salts and vitamins in admixture with from about five to about 40 parts by weight of an anion-exchange resin per part by weight of water-soluble lipid source in the finished medium and maintaining said medium at an incubation temperature for said organisms until substantial multiplication of the organisms has occurred.
Claim 5
Dependent Claim
15 words
The method of claim 5 wherein the anion-exchange resin is a weakly basic anion-exchange resin.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-06-10 | 1977-12-10 | 1978-06-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-06-10 | 1981-12-10 | 1982-06-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-06-10 | 1985-12-10 | 1986-06-11 | $7700.00 | Window Closed / Lapsed |