US20080193418A1
Expired / Lapsed
US
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Bacteriophages that Infect Bacillus Bacteria (Anthrax)
Assignee: University of Northern Iowa Foundation
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Filing: 2005-06-06
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Granted: —
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Expires: 2025-06-06
Abstract
Abstract The invention provides bacteriophages that infect Bacillus bacteria, including Bacillus anthracis , and compositions containing the bacteriophages. The invention also provides methods for using the bacteriophages of the invention to detect, prevent and treat infection of an organism by Bacillus bacteria. Methods and materials to decontaminate a surface or an organism that is contaminated with Bacillus bacteria or Bacillus spores are also provided.
Inventors
- 👤 Michael H. Walter
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 16:32
CPC Classifications
A; A61; A61K; A61K51/00; A61K51/12; A61K51/1203; C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; G; G01; G01N; G01N2333/00; G01N2333/195; G01N2333/32
Claims Analysis
Total Claims: 63
Claim 1
Independent Claim
45 words
1 . An isolated SBP1a bacteriophage, a culture of which was deposited with the American Type Culture Collection as ATCC Accession No. PTA-5057 or an isolated SBP8a bacteriophage, a culture of which was deposited with the American Type Culture Collection as ATCC Accession No. PTA-5072.
Claim 2
Dependent Claim
45 words
1 . An isolated SBP1a bacteriophage, a culture of which was deposited with the American Type Culture Collection as ATCC Accession No. PTA-5057 or an isolated SBP8a bacteriophage, a culture of which was deposited with the American Type Culture Collection as ATCC Accession No. PTA-5072.
Claim 3
Dependent Claim
21 words
2 . The bacteriophage of claim 1 , wherein the bacteriophage is coupled to a detectable marker or a solid substrate.
Claim 4
Dependent Claim
25 words
3 . The bacteriophage of claim 2 , wherein the detectable marker is an enzyme, a fluorescent tag, a radioactive tag or a colorimetric tag.
Claim 5
Dependent Claim
33 words
4 . The bacteriophage of claim 1 , wherein the bacteriophage can lyse B. anthracis strains AMES-1-RIID, AMES-RIID, ANR-1, 240, 937, 4229, 4728, 6602, 6603, 8705, 9660, 11949, 11966, 14578, 14187 or 14185.
Claim 6
Dependent Claim
26 words
5 . A pharmaceutical composition comprising a SBP1a bacteriophage (ATCC Accession No. PTA-5057) or a SBP8a bacteriophage (ATCC Accession No. PTA-5072), and a pharmaceutically acceptable carrier.
Claim 7
Dependent Claim
26 words
5 . A pharmaceutical composition comprising a SBP1a bacteriophage (ATCC Accession No. PTA-5057) or a SBP8a bacteriophage (ATCC Accession No. PTA-5072), and a pharmaceutically acceptable carrier.
Claim 8
Dependent Claim
24 words
6 . The composition of claim 5 , wherein the composition is formulated as an aerosol, a paste, a powder, or an injectable formulation.
Claim 9
Dependent Claim
40 words
7 . The pharmaceutical composition of claim 5 , wherein the composition further comprises a bacteriophage selected from NikoA (ATCC accession number PTA-4171), DDBa (ATCC accession number PTA-4172), MHWa (ATCC accession number PTA-4173), a combination or a recombinant form thereof
Claim 10
Dependent Claim
26 words
8 . An isolated antibody that can bind to a bacteriophage selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 11
Dependent Claim
26 words
8 . An isolated antibody that can bind to a bacteriophage selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 12
Dependent Claim
28 words
9 . The antibody of claim 8 , wherein the antibody is a monoclonal antibody, a polyclonal antibody, a single-chain antibody, a chimeric antibody or a humanized antibody.
Claim 13
Dependent Claim
21 words
10 . The antibody of claim 8 , wherein the antibody is coupled to a detectable marker or a solid substrate.
Claim 14
Dependent Claim
49 words
11 . A method to decontaminate a surface that is contaminated by a Bacillus anthracis bacterium or a Bacillus anthracis spore comprising applying to the surface a composition comprising a carrier and a bacteriophage selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 15
Dependent Claim
49 words
11 . A method to decontaminate a surface that is contaminated by a Bacillus anthracis bacterium or a Bacillus anthracis spore comprising applying to the surface a composition comprising a carrier and a bacteriophage selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 16
Dependent Claim
39 words
12 . The method of claim 11 , wherein the composition further comprises a bacteriophage selected from NikoA (ATCC accession number PTA-4171), DDBa (ATCC accession number PTA-4172), MHWa (ATCC accession number PTA4173), a combination or a recombinant form thereof.
Claim 17
Dependent Claim
27 words
13 . The method of claim 11 , wherein the composition kills the Bacillus anthracis bacterium or Bacillus anthracis bacterium that germinated from the Bacillus anthracis spore.
Claim 18
Dependent Claim
33 words
14 . The method of claim 11 , wherein the composition can lyse B. anthracis strains AMES-1-RIID, AMES-RIID, ANR-1, 240, 937, 4229, 4728, 6602, 6603, 8705, 9660, 11949, 11966, 14578, 14187 or 14185.
Claim 19
Dependent Claim
49 words
15 . A method to decontaminate an organism that has been contacted with Bacillus anthracis or with a Bacillus anthracis spore comprising administering to the organism a composition comprising a carrier and a bacteriophage selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 20
Dependent Claim
49 words
15 . A method to decontaminate an organism that has been contacted with Bacillus anthracis or with a Bacillus anthracis spore comprising administering to the organism a composition comprising a carrier and a bacteriophage selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 21
Dependent Claim
39 words
16 . The method of claim 15 , wherein the composition further comprises a bacteriophage selected from NikoA (ATCC accession number PTA-4171), DDBa (ATCC accession number PTA-4172), MHWa (ATCC accession number PTA-4173), a combination or a recombinant form thereof.
Claim 22
Dependent Claim
28 words
17 . The method of claim 15 , wherein the composition is inhaled by the organism, ingested by the organism, or applied to a surface of the organism.
Claim 23
Dependent Claim
14 words
18 . The method of claim 15 , wherein the organism is a mammal.
Claim 24
Dependent Claim
14 words
19 . The method of claim 15 , wherein the mammal is a human.
Claim 25
Dependent Claim
33 words
20 . The method of claim 15 , wherein the composition can lyse B. anthracis strains AMES-1-RIID, AMES-RIID, ANR-1, 240, 937, 4229, 4728, 6602, 6603, 8705, 9660, 11949, 11966, 14578, 14187 or 14185.
Claim 26
Dependent Claim
42 words
21 . A method for treating or preventing a Bacillus infection in an organism comprising administering to the organism a composition comprising a carrier and a bacteriophage selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 27
Dependent Claim
42 words
21 . A method for treating or preventing a Bacillus infection in an organism comprising administering to the organism a composition comprising a carrier and a bacteriophage selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 28
Dependent Claim
39 words
22 . The method of claim 21 , wherein the composition further comprises a bacteriophage selected from NikoA (ATCC accession number PTA-4171), DDBa (ATCC accession number PTA-4172), MHWa (ATCC accession number PTA-4173), a combination or a recombinant form thereof.
Claim 29
Dependent Claim
21 words
23 . The method of claim 21 , wherein the Bacillus is a Bacillus anthracis bacterium or a Bacillus anthracis spore.
Claim 30
Dependent Claim
14 words
24 . The method of claim 21 , wherein the organism is a mammal.
Claim 31
Dependent Claim
14 words
25 . The method of claim 21 , wherein the organism is a human.
Claim 32
Dependent Claim
89 words
26 . A method to detect Bacillus in a sample comprising: (a) contacting the sample with two or more bacteriophages that are incorporated into an electrical circuit, wherein binding of the bacterium or the bacterial spore to two or more bacteriophages changes an electrical characteristic, and (b) detecting the changed electrical characteristic to indicate that a bacterium or a bacterial spore was present in the sample; (c) wherein the two or more bacteriophages are selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 33
Dependent Claim
89 words
26 . A method to detect Bacillus in a sample comprising: (a) contacting the sample with two or more bacteriophages that are incorporated into an electrical circuit, wherein binding of the bacterium or the bacterial spore to two or more bacteriophages changes an electrical characteristic, and (b) detecting the changed electrical characteristic to indicate that a bacterium or a bacterial spore was present in the sample; (c) wherein the two or more bacteriophages are selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 34
Dependent Claim
21 words
27 . The method of claim 26 , wherein the Bacillus is a Bacillus anthracis bacterium or a Bacillus anthracis spore.
Claim 35
Dependent Claim
82 words
28 . A method to determine if a sample contains Bacillus comprising: (a) contacting the sample with an antibody and a bacteriophage, wherein the bacteriophage binds to the Bacillus and the antibody binds to the bacteriophage; (b) treating the sample to separate unbound antibody from bound antibody; and (c) detecting the presence of bound antibody to determine whether the sample contains a Bacillus; wherein the bacteriophage is selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 36
Dependent Claim
82 words
28 . A method to determine if a sample contains Bacillus comprising: (a) contacting the sample with an antibody and a bacteriophage, wherein the bacteriophage binds to the Bacillus and the antibody binds to the bacteriophage; (b) treating the sample to separate unbound antibody from bound antibody; and (c) detecting the presence of bound antibody to determine whether the sample contains a Bacillus; wherein the bacteriophage is selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 37
Dependent Claim
21 words
29 . The method of claim 28 , wherein the Bacillus is a Bacillus anthracis bacterium or a Bacillus anthracis spore.
Claim 38
Dependent Claim
72 words
30 . A method to determine whether or not a sample contains a bacterium or a bacterial spore comprising: (a) contacting the sample with a liquid crystal to which a bacteriophage is bound; and (b) determining whether or not a signal results from binding of the bacteriophage with a bacterium or bacterial spore wherein the bacteriophage is selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 39
Dependent Claim
72 words
30 . A method to determine whether or not a sample contains a bacterium or a bacterial spore comprising: (a) contacting the sample with a liquid crystal to which a bacteriophage is bound; and (b) determining whether or not a signal results from binding of the bacteriophage with a bacterium or bacterial spore wherein the bacteriophage is selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 40
Dependent Claim
53 words
31 . A method to determine whether or not a sample contains a bacterium or a bacterial spore comprising: (a) contacting the sample with a quartz crystal to which a bacteriophage is bound; and (b) determining whether or not a signal results from binding of the bacteriophage with a bacterium or bacterial spore.
Claim 41
Dependent Claim
53 words
31 . A method to determine whether or not a sample contains a bacterium or a bacterial spore comprising: (a) contacting the sample with a quartz crystal to which a bacteriophage is bound; and (b) determining whether or not a signal results from binding of the bacteriophage with a bacterium or bacterial spore.
Claim 42
Dependent Claim
27 words
32 . The method of claim 31 , wherein the bacteriophage is selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 43
Dependent Claim
101 words
33 . An apparatus for detecting a Bacillus comprising: (a) a mounting surface to which two or more bacteriophages are bound, wherein the bacteriophages can bind to the bacterium or the bacterial spore; (b) means for providing an electrical signal to two or more bacteriophages, wherein contact of the bacterium or the bacterial spore with the two or more bacteriophages bound to the mounting surface changes an electrical characteristic; and (c) a means for detecting the changed electrical characteristic. wherein the two or more bacteriophages are selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 44
Dependent Claim
101 words
33 . An apparatus for detecting a Bacillus comprising: (a) a mounting surface to which two or more bacteriophages are bound, wherein the bacteriophages can bind to the bacterium or the bacterial spore; (b) means for providing an electrical signal to two or more bacteriophages, wherein contact of the bacterium or the bacterial spore with the two or more bacteriophages bound to the mounting surface changes an electrical characteristic; and (c) a means for detecting the changed electrical characteristic. wherein the two or more bacteriophages are selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 45
Dependent Claim
21 words
34 . The apparatus of claim 33 , wherein the Bacillus is a Bacillus anthracis bacterium or a Bacillus anthracis spore.
Claim 46
Dependent Claim
14 words
35 . The apparatus of claim 33 , wherein the mounting surface is quartz.
Claim 47
Dependent Claim
37 words
36 . An apparatus comprising: (a) a liquid crystal; and (b) a bacteriophage bound to the liquid crystal; wherein the bacteriophage is selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 48
Dependent Claim
37 words
36 . An apparatus comprising: (a) a liquid crystal; and (b) a bacteriophage bound to the liquid crystal; wherein the bacteriophage is selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 49
Dependent Claim
23 words
37 . The apparatus of claim 36 , wherein the liquid crystal is a twisted nematic liquid crystal or a thermotropic liquid crystal.
Claim 50
Dependent Claim
26 words
38 . The apparatus of claim 36 , further comprising: c) a means for transducing a signal produced by binding of the bacteriophage to a target.
Claim 51
Dependent Claim
37 words
39 . The apparatus of claim 36 , wherein the apparatus comprises another bacteriophage selected from NikoA (ATCC accession number PTA-4171), DDBa (ATCC accession number PTA-4172) and MHWa (ATCC accession number PTA-4173), or a recombinant form thereof.
Claim 52
Dependent Claim
18 words
40 . An apparatus comprising: (a) a quartz crystal; and (b) a bacteriophage bound to the liquid crystal.
Claim 53
Dependent Claim
18 words
40 . An apparatus comprising: (a) a quartz crystal; and (b) a bacteriophage bound to the liquid crystal.
Claim 54
Dependent Claim
30 words
41 . The apparatus of claim 40 , further comprising: c) a means for transducing a signal produced by binding of a bacterium or a bacterial spore to the bacteriophage.
Claim 55
Dependent Claim
47 words
42 . The apparatus of claim 40 , wherein the bacteriophage is selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057), SBP8a bacteriophage (ATCC Accession No. PTA-5072), NikoA (ATCC accession number PTA-4171), DDBa (ATCC accession number PTA-4172) and MHWa (ATCC accession number PTA-4173), or a recombinant form thereof.
Claim 56
Dependent Claim
27 words
43 . A biosensor comprising a detector operatively coupled to a bacteriophage selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 57
Dependent Claim
27 words
43 . A biosensor comprising a detector operatively coupled to a bacteriophage selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 58
Dependent Claim
34 words
44 . The biosensor of statement 43, wherein the detector is a piezoelectric device, an acoustic wave device, a surface plasmon resonance device, an optical fiber device or a light addressable potentiometric sensor device.
Claim 59
Dependent Claim
34 words
44 . The biosensor of statement 43, wherein the detector is a piezoelectric device, an acoustic wave device, a surface plasmon resonance device, an optical fiber device or a light addressable potentiometric sensor device.
Claim 60
Dependent Claim
40 words
45 . A biosensor comprising solid substrate and an antibody operatively coupled to the solid substrate, wherein the antibody can bind a bacteriophage that is selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 61
Dependent Claim
40 words
45 . A biosensor comprising solid substrate and an antibody operatively coupled to the solid substrate, wherein the antibody can bind a bacteriophage that is selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 62
Dependent Claim
22 words
46 . A kit comprising a bacteriophage selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
Claim 63
Dependent Claim
22 words
46 . A kit comprising a bacteriophage selected from SBP1a bacteriophage (ATCC Accession No. PTA-5057) or SBP8a bacteriophage (ATCC Accession No. PTA-5072).
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