US7195909B2
Expired / Lapsed
US
⏱️ 0.0 years remaining
Measurement and use of molecular interactions
Assignee: Alere Switzerland GmbH
•
Filing: 2003-01-24
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Granted: 2007-03-27
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Expires: 2023-01-24
Abstract
Abstract The subject invention pertains to a method for determining the affinity between binding partners, or a property of one of the binding partners dependent on the affinity, comprising the steps of: (i) contacting the binding partners, one of which is immobilised on a surface; (ii) oscillating the surface at increasing amplitude; and (iii) detecting a dissociation event. An analogous method can be used to separate a target analyte from a composition. The subject invention also pertains to an apparatus for determining the affinity between binding partners, and comprises: a surface ( 10 ) having one binding partner ( 16 ) immobilised thereon; means for oscillating the surface at increasing amplitude; and means ( 14, 15 ) for detecting a dissociation event.
Inventors
- 👤 David Klenerman
- 👤 Victor Petrovich Ostanin
- 👤 Fedor Nikolaievich Dultsev
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 05:38
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N33/5438; Y; Y10; Y10S; Y10S435/00; Y10S435/808; Y10S436/00; Y10S436/805
Claims Analysis
Total Claims: 38
Claim 1
Independent Claim
48 words
An apparatus suitable for detecting the dissociation of a bond, which comprises: (i) a surface on which the bond may be formed; (ii) a means for oscillating said surface at monotonously increasing amplitude in the plane of said surface, and (iii) a means for detecting a dissociation event.
Claim 2
Independent Claim
48 words
An apparatus suitable for detecting the dissociation of a bond, which comprises: (i) a surface on which the bond may be formed; (ii) a means for oscillating said surface at monotonously increasing amplitude in the plane of said surface, and (iii) a means for detecting a dissociation event.
Claim 3
Dependent Claim
16 words
The apparatus of claim 1 , wherein one or more ligands are immobilized on the surface.
Claim 4
Dependent Claim
27 words
The apparatus according to claim 2 , wherein said one or more immobilized ligands are selected from the group consisting of cells, bacteria, viruses, prions and phages.
Claim 5
Dependent Claim
18 words
The apparatus according to claim 2 , wherein different ligands are immobilized at different positions on said surface.
Claim 6
Dependent Claim
29 words
The apparatus according to claim 1 , wherein said one or more immobilized ligands are selected from the group consisting of proteins, antibodies, antigens, enzymes, enzyme inhibitors and polynucleotides.
Claim 7
Dependent Claim
14 words
The apparatus according to claim 1 , wherein said detecting means detects acoustic emission.
Claim 8
Dependent Claim
22 words
The apparatus according to claim 1 , wherein said oscillating means and said detecting means comprise a piezoelectric or an acoustic transducer.
Claim 9
Dependent Claim
16 words
The apparatus according to claim 7 , wherein said transducer is a surface acoustic wave device.
Claim 10
Dependent Claim
16 words
The apparatus according to claim 7 , wherein said transducer is a surface crystal microbalance (QCM).
Claim 11
Dependent Claim
36 words
The apparatus according to claim 9 , wherein said oscillating means drives the QCM at its resonance frequency, and said detecting means is sensitive to the acoustic emission at the third harmonic of the driving frequency.
Claim 12
Dependent Claim
29 words
The apparatus according to claim 10 , wherein said oscillating means causes oscillation of the surface at one resonant frequency and said detecting means detects dissociation at another frequency.
Claim 13
Dependent Claim
15 words
The apparatus according to claim 11 , wherein said another frequency is another resonant frequency.
Claim 14
Dependent Claim
16 words
The apparatus according to claim 11 , wherein said oscillation frequency is the fundamental resonant frequency.
Claim 15
Dependent Claim
17 words
The apparatus according to claim 13 , wherein said another frequency is the third overtone resonant frequency.
Claim 16
Dependent Claim
36 words
The apparatus according to claim 11 , wherein said oscillating means drives the QCM at its resonance frequency, and said detecting means is sensitive to the acoustic emission at the third harmonic of the driving frequency.
Claim 17
Dependent Claim
29 words
The apparatus according to claim 1 , wherein said oscillating means causes oscillation of the surface at one resonant frequency and said detecting means detects dissociation at another frequency.
Claim 18
Dependent Claim
15 words
The apparatus according to claim 16 , wherein said another frequency is another resonant frequency.
Claim 19
Dependent Claim
16 words
The apparatus according to claim 16 , wherein said oscillation frequency is the fundamental resonant frequency.
Claim 20
Dependent Claim
17 words
The apparatus according to claim 18 , wherein said another frequency is the third overtone resonant frequency.
Claim 21
Independent Claim
61 words
An apparatus suitable for detecting the dissociation of a bond, which comprises: (i) a surface on which the bond may be formed; (ii) a means for oscillating said surface at increasing amplitude in the plane of said surface; and (iii) a means for detecting a change in amplitude of another, simultaneous oscillation of said oscillating means caused by a dissociation event.
Claim 22
Independent Claim
61 words
An apparatus suitable for detecting the dissociation of a bond, which comprises: (i) a surface on which the bond may be formed; (ii) a means for oscillating said surface at increasing amplitude in the plane of said surface; and (iii) a means for detecting a change in amplitude of another, simultaneous oscillation of said oscillating means caused by a dissociation event.
Claim 23
Dependent Claim
16 words
The apparatus of claim 20 , wherein one or more ligands are immobilized on the surface.
Claim 24
Dependent Claim
29 words
The apparatus according to claim 21 , wherein said one or more immobilized ligands are selected from the group consisting of proteins, antibodies, antigens, enzymes, enzyme inhibitors and polynucleotides.
Claim 25
Dependent Claim
27 words
The apparatus according to claim 21 , wherein said one or more immobilized ligands are selected from the group consisting of cells, bacteria, viruses, prions and phages.
Claim 26
Dependent Claim
18 words
The apparatus according to claim 21 , wherein different ligands are immobilized at different positions on the surface.
Claim 27
Dependent Claim
14 words
The apparatus according to claim 20 , wherein said detecting means detects acoustic emission.
Claim 28
Dependent Claim
22 words
The apparatus according to claim 20 , wherein said oscillating means and said detecting means comprise a piezoelectric or an acoustic transducer.
Claim 29
Dependent Claim
16 words
The apparatus according to claim 26 , wherein said transducer is a surface acoustic wave device.
Claim 30
Dependent Claim
16 words
The apparatus according to claim 26 , wherein said transducer is a quartz crystal microbalance (QCM).
Claim 31
Dependent Claim
14 words
The apparatus according to claim 20 , wherein said increasing amplitude is monotonously increasing.
Claim 32
Independent Claim
70 words
An apparatus suitable for detecting the dissociation of a bond, which comprises: (i) a surface on which the bond may be formed; (ii) a means for oscillating said surface at monotonously increasing amplitude in the plane of said surface, and (iii) a means for detecting a dissociation event; wherein the means for detecting the dissociation event comprises detecting a signal other than the signal driving the oscillation of the surface.
Claim 33
Independent Claim
70 words
An apparatus suitable for detecting the dissociation of a bond, which comprises: (i) a surface on which the bond may be formed; (ii) a means for oscillating said surface at monotonously increasing amplitude in the plane of said surface, and (iii) a means for detecting a dissociation event; wherein the means for detecting the dissociation event comprises detecting a signal other than the signal driving the oscillation of the surface.
Claim 34
Dependent Claim
14 words
The apparatus, according to claim 30 , wherein said detecting means detects acoustic emission.
Claim 35
Independent Claim
55 words
An apparatus suitable for detecting the dissociation of a bond, which comprises: (i) a surface on which the bond may be formed; (ii) a means for oscillating said surface at monotonously increasing amplitude in the plane of said surface, and (iii) a means for detecting a dissociation event; wherein said detecting means detects acoustic emission.
Claim 36
Independent Claim
55 words
An apparatus suitable for detecting the dissociation of a bond, which comprises: (i) a surface on which the bond may be formed; (ii) a means for oscillating said surface at monotonously increasing amplitude in the plane of said surface, and (iii) a means for detecting a dissociation event; wherein said detecting means detects acoustic emission.
Claim 37
Independent Claim
55 words
An apparatus suitable for detecting the dissociation of a bond, which comprises: (i) a surface on which the bond may be formed; (ii) a means for oscillating said surface at monotonously increasing amplitude in the plane of said surface, and (iii) a means for detecting a dissociation event; wherein said detecting means detects optical emissions.
Claim 38
Independent Claim
55 words
An apparatus suitable for detecting the dissociation of a bond, which comprises: (i) a surface on which the bond may be formed; (ii) a means for oscillating said surface at monotonously increasing amplitude in the plane of said surface, and (iii) a means for detecting a dissociation event; wherein said detecting means detects optical emissions.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2010-03-26 | 2010-09-25 | 2011-03-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2014-03-26 | 2014-09-25 | 2015-03-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2018-03-26 | 2018-09-25 | 2019-03-27 | $7700.00 | Window Closed / Lapsed |