US7267993B2
Expired / Lapsed
US
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Phage ligand sensor devices and uses thereof
Assignee: Auburn University
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Filing: 2002-11-07
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Granted: 2007-09-11
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Expires: 2022-11-07
Abstract
Abstract Methods and compositions for identifying and characterizing one or more ligands of a peptide are provided. In particular, the invention provides a phage ligand sensor device (PLSD) comprising a sensor coupled to a binding element of interest. Binding elements of interest comprise phage displaying at least one foreign peptide. The PLSD and assays find particular use in identifying and characterizing ligand-peptide interactions.
Inventors
- 👤 Valery A. Pentrenko
- 👤 Vitaly J. Vodyanoy
- 👤 Alexandre M. Samoylov
- 👤 Iryna Sorokulova
- 👤 Viswaprakash Nanduri
- 👤 Bryan A. Chin
- 👤 James M. Barbaree
- 👤 W. Charles Neely
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 19:21
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N33/569
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
114 words
A phage ligand sensor device comprising: a) a sensor comprising a piezoelectric crystal; and b) a binding element comprising engineered phage, wherein said binding element is capable of binding to a ligand that is Bacillus anthracis bacteria or spores, wherein said phage have been engineered to express a foreign peptide known to bind to said Bacillus anthracis bacteria or spores, and wherein said foreign peptide comprises an amino acid sequence selected from the group consisting of DPRTVSTA (SEQ ID NO:8), DRTSSNTT (SEQ ID NO:9), DRSASSTA (SEQ ID NO:10), DRTSQNLQ (SEQ ID NO:11), DRSGTSPS (SEQ ID NO:12), DRNVNGPS (SEQ ID NO:13), ERGSNATL (SEQ ID NO:14), DSRNEKSM (SEQ ID NO: 15), ESRNSPAA (SEQ ID NO: 16).
Claim 2
Dependent Claim
114 words
A phage ligand sensor device comprising: a) a sensor comprising a piezoelectric crystal; and b) a binding element comprising engineered phage, wherein said binding element is capable of binding to a ligand that is Bacillus anthracis bacteria or spores, wherein said phage have been engineered to express a foreign peptide known to bind to said Bacillus anthracis bacteria or spores, and wherein said foreign peptide comprises an amino acid sequence selected from the group consisting of DPRTVSTA (SEQ ID NO:8), DRTSSNTT (SEQ ID NO:9), DRSASSTA (SEQ ID NO:10), DRTSQNLQ (SEQ ID NO:11), DRSGTSPS (SEQ ID NO:12), DRNVNGPS (SEQ ID NO:13), ERGSNATL (SEQ ID NO:14), DSRNEKSM (SEQ ID NO: 15), ESRNSPAA (SEQ ID NO: 16).
Claim 3
Dependent Claim
24 words
The phage ligand sensor device of claim 1 , wherein said binding element is coupled to said sensor by a layer of biotinylated lipid.
Claim 4
Dependent Claim
24 words
The phage ligand sensor device of claim 1 , wherein said foreign peptide comprises an amino acid sequence of the β-galactosidase amino acid sequence.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2010-09-10 | 2011-03-12 | 2011-09-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2014-09-10 | 2015-03-12 | 2015-09-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2018-09-10 | 2019-03-12 | 2019-09-11 | $7700.00 | Window Closed / Lapsed |