US4459359A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Sensitive immunoassays of antigens or antibodies sequestered within immune complexes
Assignee: New York Blood Center
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Filing: 1981-11-19
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Granted: 1984-07-10
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Expires: 2001-11-19
Abstract
Abstract A process for determining the presence of an antigen or antibody in a sample wherein said antigen or antibody exists in the form of an immune complex which comprises: A. contacting the immune complex originating from the sample suspected of containing immune complex with a dissociating buffer whereby said immune complex, if present, is dissociated into antigen and antibody; B. contacting a solid support which binds proteins with said dissociating buffer suspected of containing antigen or antibody and removing said buffer; C. washing said solid support; D. adding protein to fill unoccupied sites on said solid support; E. adding radioactively labeled or enzyme labeled antibody or antigen to said solid support, said labeled antibody or antigen corresponding to antigen or antibody on said solid support, incubating the resultant mass and washing the same; F. measuring the radioactivity or enzymatic activity associated with the solid support.
Inventors
- 👤 A. Robert Neurath
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 07:28
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/54306; G01N33/54393; G01N33/564; G01N33/576; G01N33/5761; Y; Y10; Y10S; Y10S435/00; Y10S435/962; Y10S436/00; Y10S436/804; Y10S436/825; Y10S436/828
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
145 words
A process for determining the presence of an antigen or antibody in a sample wherein said antigen or antibody exists in the form of an immune complex which comprises: A. contacting the immune complex originating from the sample suspected of containing immune complex with a dissociating buffer whereby said immune complex, if present, is dissociated into antigen and antibody; B. contacting a solid support which binds proteins with said dissociating buffer suspected of containing antigen or antibody and removing said buffer; C. washing said solid support; D. adding protein to fill unoccupied sites on said solid support; E. adding radioactively labeled or enzyme labeled antibody or antigen to said solid support, said labeled antibody or antigen corresponding to antigen or antibody on said solid support, incubating the resultant mass and washing the same; F. measuring the radioactivity or enzymatic activity associated with the solid support.
Claim 2
Dependent Claim
145 words
A process for determining the presence of an antigen or antibody in a sample wherein said antigen or antibody exists in the form of an immune complex which comprises: A. contacting the immune complex originating from the sample suspected of containing immune complex with a dissociating buffer whereby said immune complex, if present, is dissociated into antigen and antibody; B. contacting a solid support which binds proteins with said dissociating buffer suspected of containing antigen or antibody and removing said buffer; C. washing said solid support; D. adding protein to fill unoccupied sites on said solid support; E. adding radioactively labeled or enzyme labeled antibody or antigen to said solid support, said labeled antibody or antigen corresponding to antigen or antibody on said solid support, incubating the resultant mass and washing the same; F. measuring the radioactivity or enzymatic activity associated with the solid support.
Claim 3
Dependent Claim
20 words
A process according to claim 1, wherein the solid support of step B is a derivatized paper or plastic material.
Claim 4
Dependent Claim
15 words
A process according to claim 2, wherein said solid support is a paper (cellulose) derivative.
Claim 5
Dependent Claim
13 words
A process according to claim 3, wherein said paper is a nitrocellulose paper.
Claim 6
Dependent Claim
17 words
A process according to claim 3, wherein said paper is a diazobenzyloxymethyl paper or a diazophenylthioether paper.
Claim 7
Dependent Claim
14 words
A process according to claim 2, wherein said solid support is a plastic material.
Claim 8
Dependent Claim
12 words
A process according to claim 6, wherein said plastic is a polystyrene.
Claim 9
Dependent Claim
13 words
A process according to claim 6, wherein said plastic is a polyvinyl material.
Claim 10
Dependent Claim
12 words
A process according to claim 6, wherein said plastic is a polyolefin.
Claim 11
Dependent Claim
26 words
A process according to claim 1, wherein the solid support of step B is a paper derivatized and step D is performed prior to step E.
Claim 12
Dependent Claim
14 words
A process according to claim 6, wherein steps D and E are performed simultaneously.
Claim 13
Dependent Claim
26 words
A process according to claim 1, wherein step E is performed using a radiolabeled antigen or antibody and step F is performed using radio-immunoassay or autoradiography.
Claim 14
Dependent Claim
29 words
A process according to claim 1, wherein step E is performed using an enzyme labeled antibody or antigen and step F is performed using an enzyme labeled immunoassay method.
Claim 15
Dependent Claim
67 words
A process according to claim 1, wherein prior to step A a mixture suspected of containing immune complex is brought in contact with a separating support which binds proteins whereby said immune complex, if present, is adsorbed to said separating support and thereby separated from materials with which it has been in admixture and thereafter the dissociating buffer is added to said separating support containing immune complex.
Claim 16
Dependent Claim
64 words
A process according to claim 14, wherein the sample suspected of containing immune complex is initially treated with a selective protein precipitating agent whereby immune complexes are precipitated if present. Precipitate is separated from supernatant material and the immune complex is taken up in solution by contacting the precipitate with a neutral, non-dissociating buffer prior to being brought in contact with said separating support.
Claim 17
Dependent Claim
15 words
A process according to claim 14, wherein said separating support comprises Staphylococci carrying proteint (A).
Claim 18
Dependent Claim
15 words
A process according to claim 15, wherein said separating support comprises Staphylococci carrying protein (A).
Claim 19
Dependent Claim
20 words
A process according to claim 1, wherein said buffer comprises an alkali or alkaline earth metal thiocyanate of pH 6-8.
Claim 20
Dependent Claim
20 words
A process according to claim 14, wherein said buffer comprises an alkali or alkaline earth metal thiocyanate of pH 6-8.
Claim 21
Dependent Claim
20 words
A process according to claim 15, wherein said buffer comprises an alkali or alkaline earth metal thiocyanate of pH 6-8.
Claim 22
Dependent Claim
18 words
A process according to claim 1, wherein said sample contains an immune complex of Hepatitis B (surface) antigen.
Claim 23
Dependent Claim
18 words
A process according to claim 14, wherein said sample contains an immune complex of Hepatitis B (surface) antigen.
Claim 24
Dependent Claim
18 words
A process according to claim 15, wherein said sample contains an immune complex of Hepatitis B (surface) antigen.
References Cited (Backward Citations)
US3872225A
Cited
US4138213A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-07-10 | 1988-01-09 | 1988-07-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-07-10 | 1992-01-09 | 1992-07-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-07-10 | 1996-01-09 | 1996-07-10 | $7700.00 | Window Closed / Lapsed |