US7543476B2
Expired / Lapsed
US
⏱️ 0.0 years remaining
Rupture event sensors
Assignee: Alere Switzerland GmbH
•
Filing: 2004-04-14
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Granted: 2009-06-09
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Expires: 2024-04-14
Abstract
Abstract An apparatus for separating an analyte from a mixture or for detecting an analyte or for determining the affinity, or a property related to affinity, between binding partners includes: a) a surface having the analyte or one of the binding partners immobilized thereon, in use; b) a transducer for oscillating the surface; c) a controller connected to the transducer for varying the amplitude and/or frequency of the oscillation to cause a dissociation event; and, d) an analyzer connected to the transducer for detecting an oscillation of the transducer due to the dissociation event. The controller includes an oscillator connected in a resonant circuit with the transducer such that the transducer oscillates at two frequencies simultaneously, one of these causing the transducer to oscillate the surface and the other being supplied as an output to the analyzer.
Inventors
- 👤 Victor Petrovich Ostanin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 21:22
CPC Classifications
G; G01; G01N; G01N29/00; G01N29/02; G01N29/036; H; H03; H03B; H03B21/00; H03B5/00; H03B5/30; H03B5/32; H03B5/36; H03B5/366; H03B5/368; H03L; H03L5/00; G01N2291/00; G01N2291/02; G01N2291/025; G01N2291/0256
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
133 words
Apparatus for separating an analyte from a mixture or for detecting an analyte or for determining the affinity, or a property related to affinity, between binding partners comprising: a) a surface having the analyte or one of the binding partners immobilised thereon, in use; b) a transducer for oscillating the surface; c) a controller connected to the transducer for varying the amplitude, frequency, or amplitude and frequency of the oscillation to cause a dissociation event; and, d) an analyser connected to the transducer for detecting an oscillation of the transducer due to the dissociation event; characterised in that the controller includes an oscillator connected in a resonant circuit with the transducer such that the transducer oscillates at first and second frequencies simultaneously, the second frequency being supplied as an output to the analyser.
Claim 2
Dependent Claim
133 words
Apparatus for separating an analyte from a mixture or for detecting an analyte or for determining the affinity, or a property related to affinity, between binding partners comprising: a) a surface having the analyte or one of the binding partners immobilised thereon, in use; b) a transducer for oscillating the surface; c) a controller connected to the transducer for varying the amplitude, frequency, or amplitude and frequency of the oscillation to cause a dissociation event; and, d) an analyser connected to the transducer for detecting an oscillation of the transducer due to the dissociation event; characterised in that the controller includes an oscillator connected in a resonant circuit with the transducer such that the transducer oscillates at first and second frequencies simultaneously, the second frequency being supplied as an output to the analyser.
Claim 3
Dependent Claim
26 words
Apparatus according to claim 1 , wherein the second frequency that is supplied as an output to the analyser is a multiple of the first frequency.
Claim 4
Dependent Claim
34 words
Apparatus according to claim 2 , wherein the first frequency is the transducer's fundamental resonant frequency and the second frequency supplied as an output to the analyser is one of the transducer's overtone frequencies.
Claim 5
Dependent Claim
26 words
Apparatus according to claim 1 , wherein the first frequency is a multiple of the second frequency that is supplied as an output to the analyser.
Claim 6
Dependent Claim
33 words
Apparatus according to claim 4 , wherein the first frequency is one of the transducer's overtone frequencies and the second frequency supplied as an output to the analyser is the transducer's fundamental frequency.
Claim 7
Dependent Claim
33 words
Apparatus according to claim 1 , wherein the oscillation of the transducer due to the dissociation event is at a range of frequencies located around at least one of the transducer's resonant frequencies.
Claim 8
Dependent Claim
48 words
Apparatus according to claim 1 , wherein the immobilised analyte or binding partner is a metal, a polymer, a dendrimer, a self-assembled monolayer, a peptide, a protein, an antibody, an antigen, an enzyme, an enzyme inhibitor, a biologically active molecule, a drug, a polynucleotide or a peptide polynucleotide.
Claim 9
Dependent Claim
30 words
Apparatus according to claim 1 , wherein the immobilised analyte or binding partner is a cell, a bacterium, a virus, a prion, an amyloid, a proteinaceous aggregate or a phage.
Claim 10
Dependent Claim
16 words
Apparatus according to claim 1 , wherein the dissociation event is detected as a motional oscillation.
Claim 11
Dependent Claim
13 words
Apparatus according to claim 1 , wherein the transducer is a piezoelectric transducer.
Claim 12
Dependent Claim
14 words
Apparatus according to claim 10 , wherein the transducer is a quartz crystal microbalance.
Claim 13
Dependent Claim
18 words
Apparatus according to claim 10 , wherein the transducer comprises zinc oxide, a piezoelectric polymer or a piezo-ceramic.
Claim 14
Dependent Claim
15 words
Apparatus according to claim 10 , wherein the oscillator is a dual frequency crystal oscillator.
Claim 15
Dependent Claim
60 words
Apparatus according to claim 1 , wherein the oscillator comprises two bandpass filters, each having its input connected to the transducer and its output connected to a respective amplifier, the outputs of which are combined by a power adder and supplied to the transducer, the centre frequencies of the bandpass filters corresponding to the two oscillating frequencies of the transducer.
Claim 16
Dependent Claim
16 words
Apparatus according to claim 1 , wherein the analyser comprises a radiofrequency detector and a digitiser.
Claim 17
Independent Claim
111 words
A method for separating an analyte from a mixture or for detecting an analyte or for determining the affinity, or a property related to affinity, between binding partners, the method comprising: a) immobilising the analyte or one of the binding partners on a surface; b) oscillating the surface; c) varying the amplitude, frequency, or amplitude and frequency of the oscillation to cause a dissociation event; and, d) detecting an oscillation due to the dissociation event using an analyser; characterised by oscillating the surface at first and second frequencies simultaneously, the second frequency being supplied as an output to the analyser for use in detecting the oscillation due to the dissociation event.
Claim 18
Independent Claim
111 words
A method for separating an analyte from a mixture or for detecting an analyte or for determining the affinity, or a property related to affinity, between binding partners, the method comprising: a) immobilising the analyte or one of the binding partners on a surface; b) oscillating the surface; c) varying the amplitude, frequency, or amplitude and frequency of the oscillation to cause a dissociation event; and, d) detecting an oscillation due to the dissociation event using an analyser; characterised by oscillating the surface at first and second frequencies simultaneously, the second frequency being supplied as an output to the analyser for use in detecting the oscillation due to the dissociation event.
Claim 19
Dependent Claim
35 words
A method according to claim 16 , wherein the surface is oscillated using a transducer and the second frequency that is supplied as an output to the analyser is a multiple of the first frequency.
Claim 20
Dependent Claim
35 words
A method according to claim 17 , wherein the first frequency is the transducer's fundamental resonant frequency and the second frequency supplied as an output to the analyser is one of the transducer's overtone frequencies.
Claim 21
Dependent Claim
36 words
A method according to claim 16 , wherein the surface is oscillated using a transducer, and wherein the first frequency is a multiple of the second frequency that is supplied as an output to the analyser.
Claim 22
Dependent Claim
34 words
A method according to claim 19 , wherein the first frequency is one of the transducer's overtone frequencies and the second frequency supplied as an output to the analyser is the transducer's fundamental frequency.
Claim 23
Dependent Claim
41 words
A method according to any claim 16 , wherein the surface is oscillated using a transducer, and wherein the oscillation due to the dissociation event is at a range of frequencies located around at least one of the transducer's resonant frequencies.
Claim 24
Dependent Claim
17 words
A method according to claim 16 , further comprising detecting the dissociation event as a motional oscillation.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 2012-06-08 | 2012-12-08 | 2013-06-09 | $2000.00 | Window Closed / Lapsed |
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