US20030194697A1
Expired / Lapsed
US
⏱️ 0.0 years remaining
Measurement and use of molecular interactions
Assignee: Alere Switzerland GmbH
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Filing: 2003-01-24
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Granted: —
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Expires: 2023-01-24
Abstract
Abstract The subject invention pertains to a method for determining the affinity between binding partners or a property of one of the binding partners dependent on the affinity, comprising the step of: (i) contacting the binding partners, one of which is immobilised on a surface; (ii) oscillating the surface at increasing amplitude; and (iii) detecting a dissociation event. An analogous method can be used to separate a target analyte from a composition. The subject invention also pertains to an apparatus for determining the affinity between binding partners, and comprises: a surface ( 10 ) having one binding partner ( 16 ) immobilised thereon; means for oscillating the surface at increasing amplitude; and means ( 14, 15 ) for detecting a dissociation event.
Inventors
- 👤 David Klenerman
- 👤 Victor Ostanin
- 👤 Fedor Dultsev
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 22:40
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N33/5438; Y; Y10; Y10S; Y10S435/00; Y10S435/808; Y10S436/00; Y10S436/805
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
80 words
1 . An apparatus for determining the affinity between first and second binding partners, which comprises: (i) a surface to which one of the binding partners can be immobilised thereon; (ii) a means for oscillating the surface at increasing amplitude in the plane of the surface; and (iii) a means for detecting dissociation of bound binding partners that results from oscillating the surface at increasing amplitude, wherein affinity of the first and second binding partners is determined from the dissociation.
Claim 2
Dependent Claim
80 words
1 . An apparatus for determining the affinity between first and second binding partners, which comprises: (i) a surface to which one of the binding partners can be immobilised thereon; (ii) a means for oscillating the surface at increasing amplitude in the plane of the surface; and (iii) a means for detecting dissociation of bound binding partners that results from oscillating the surface at increasing amplitude, wherein affinity of the first and second binding partners is determined from the dissociation.
Claim 3
Dependent Claim
29 words
2 . The apparatus according to claim 1 , wherein the immobilised binding partner is a protein, antibody, antigen, enzyme, enzyme inhibitor, polynucleotide, cell, bacterium, virus, prion or phage.
Claim 4
Dependent Claim
21 words
3 . The apparatus according to claim 2 , wherein different binding partners are immobilised at different positions on the surface.
Claim 5
Dependent Claim
16 words
4 . The apparatus according to claim 1 , wherein the detecting means detects acoustic emission.
Claim 6
Dependent Claim
24 words
5 . The apparatus according to claim 1 , wherein the oscillating means and the detecting means comprises a piezoelectric or an acoustic transducer.
Claim 7
Dependent Claim
22 words
6 . The apparatus according to claim 5 , wherein the transducer is a quartz crystal microbalance or surface acoustic wave device.
Claim 8
Dependent Claim
21 words
7 . The apparatus according to claim 1 , wherein different binding partners are immobilised at different positions on the surface.
Claim 9
Dependent Claim
87 words
8 . An apparatus for determining the affinity between first and second binding partners, which comprises: (i) a surface to which one of the binding partners can be immobilised thereon; (ii) a means for oscillating the surface at increasing amplitude; and (iii) a means for detecting acoustic emission of the dissociation of bound binding partners that results from oscillating the surface at increasing amplitude, wherein affinity of the first and second binding partners is determined from the amplitude of oscillation at which the acoustic emission is detected.
Claim 10
Dependent Claim
87 words
8 . An apparatus for determining the affinity between first and second binding partners, which comprises: (i) a surface to which one of the binding partners can be immobilised thereon; (ii) a means for oscillating the surface at increasing amplitude; and (iii) a means for detecting acoustic emission of the dissociation of bound binding partners that results from oscillating the surface at increasing amplitude, wherein affinity of the first and second binding partners is determined from the amplitude of oscillation at which the acoustic emission is detected.
Claim 11
Dependent Claim
29 words
9 . The apparatus according to claim 8 , wherein the immobilised binding partner is a protein, antibody, antigen, enzyme, enzyme inhibitor, polynucleotide, cell, bacterium, virus, prion, or phage.
Claim 12
Dependent Claim
21 words
10 . The apparatus according to claim 8 , wherein different binding partners are immobilised at different positions on the surface.
Claim 13
Dependent Claim
25 words
11 . The apparatus according to claim 8 , wherein the oscillating means and the detecting means comprises a piezoelectric transducer or an acoustic transducer.
Claim 14
Dependent Claim
22 words
12 . The apparatus according to claim 11 , wherein the transducer is a quartz crystal microbalance or surface acoustic wave device.
Claim 15
Dependent Claim
46 words
13 . The apparatus according to claim 8 , wherein the oscillating means is an oscillator adapted to oscillate the surface at a frequency corresponding to one major resonance frequency, and the detecting means is a detector adapted to detect acoustic emission near to another mode.
Claim 16
Dependent Claim
48 words
14 . The apparatus according to claim 8 , wherein the oscillating means and the detecting means comprises a quartz crystal microbalance (QCM), and the oscillating means drives the QCM at its resonance frequency, and the detecting means is sensitive to the acoustic emission at its third harmonic.
Claim 17
Dependent Claim
83 words
15 . A method for detecting dissociation of first and second binding partners, which comprises the steps of: (i) contacting the first and second binding partners, wherein the first binding partner is immobilised on a surface, and wherein the first and second binding partners bind to each other; (ii) oscillating the surface such that the amplitude of oscillation increases to a value at which the first and second binding partners dissociate; and (iii) detecting the dissociation of the first and second binding partners.
Claim 18
Dependent Claim
83 words
15 . A method for detecting dissociation of first and second binding partners, which comprises the steps of: (i) contacting the first and second binding partners, wherein the first binding partner is immobilised on a surface, and wherein the first and second binding partners bind to each other; (ii) oscillating the surface such that the amplitude of oscillation increases to a value at which the first and second binding partners dissociate; and (iii) detecting the dissociation of the first and second binding partners.
Claim 19
Dependent Claim
25 words
16 . The method according to claim 15 , wherein the first or second binding partner is a particle or is immobilised on a particle.
Claim 20
Dependent Claim
31 words
17 . The method according to claim 15 , wherein the first or second binding partner is a protein, antibody, antigen, enzyme, enzyme inhibitor, polynucleotide, cell, bacterium, virus, prion, or phage.
Claim 21
Dependent Claim
24 words
18 . The method according to claim 15 , wherein the dissociation of the first and second binding partners is detected by acoustic emission.
Claim 22
Dependent Claim
19 words
19 . The method according to claim 15 , wherein the surface is a piezoelectric transducer or acoustic transducer.
Claim 23
Dependent Claim
22 words
20 . The method according to claim 19 , wherein the transducer is a quartz crystal microbalance or surface acoustic wave device.
Claim 24
Dependent Claim
33 words
21 . The method according to claim 15 , wherein the oscillation of the surface is conducted at one resonant frequency and the detection of the dissociation is conducted at another resonant frequency.
Claim 25
Dependent Claim
27 words
22 . The method according to claim 21 , wherein the oscillation frequency is the fundamental resonant frequency and the detection frequency is the third overtone frequency.
Claim 26
Dependent Claim
29 words
23 . The method according to claim 21 , wherein the oscillation frequency is the fundamental resonant frequency and the detection frequency is at three times the fundamental frequency.
Claim 27
Dependent Claim
65 words
24 . An apparatus for detecting dissociation of first and second binding partners, which comprises: (i) a surface to which one of the binding partners can be immobilised; (ii) a means for oscillating the surface at increasing amplitude in the plane of the surface; and (iii) a means for detecting the dissociation of bound binding partners that results from oscillating the surface at increasing amplitude.
Claim 28
Dependent Claim
65 words
24 . An apparatus for detecting dissociation of first and second binding partners, which comprises: (i) a surface to which one of the binding partners can be immobilised; (ii) a means for oscillating the surface at increasing amplitude in the plane of the surface; and (iii) a means for detecting the dissociation of bound binding partners that results from oscillating the surface at increasing amplitude.
Claim 29
Dependent Claim
29 words
25 . The apparatus according to claim 33 , wherein the immobilised binding partner is a protein, antibody, antigen, enzyme, enzyme inhibitor, polynucleotide, cell, bacterium, virus, prion or phage.
Claim 30
Dependent Claim
21 words
26 . The apparatus according to claim 24 , wherein different binding partners are immobilised at different positions on the surface.
Claim 31
Dependent Claim
16 words
27 . The apparatus according to claim 24 , wherein the detecting means detects acoustic emission.
Claim 32
Dependent Claim
24 words
28 . The apparatus according to claim 27 , wherein the oscillating means and the detecting means comprises a piezoelectric or an acoustic transducer.
Claim 33
Dependent Claim
22 words
29 . The apparatus according to claim 28 , wherein the transducer is a quartz crystal microbalance or surface acoustic wave device.
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