Patent EP0177191A1 successfully refreshed from Google Patents.
EP0177191A1
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Methods of assay
Assignee: Adaltis US Inc
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Filing: 1985-09-04
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Granted: —
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Expires: 2005-09-04
Abstract
Abstract The present invention provides a method of assay of a ligand in a sample, which comprises incubating, simultaneously or in sequence, (a) the sample (containing the analyte ligand), (b) a directly labelled specific binding partner to the ligand, (c) a ligand analogue as herein defined, conjugated with a reagent X (the said reagent not being present as a free reagent in the mixture); separating, after a suitable incubation period, the portion containing component (c) from the mixture by means of a solid phase carrying a binding partner specific for reagent X; and determining the extent of complexing between components (b) and (c) by measurement of the label.
Inventors
- 👤 Gerald John Allen
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-02 18:29
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/563; G01N33/536; G01N33/537; G01N33/538
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
103 words
A method of assay of a ligand in a sample, which comprises incubating, simultaneously or in sequence, (a) the sample (containing the analyte ligand), (b) a directly labelled specific binding partner to the ligand, (c) a ligand analogue as herein defined, conjugated with a reagent X (the said reagent not being present as a free reagent in the mixture); separating, after a suitable incubation period, the portion containing component (c) from the mixture by means of a solid phase carrying a binding partner specific for reagent X; and determining the extent of complexing between components (b) and (c) by measurement of the label.
Claim 2
Independent Claim
103 words
A method of assay of a ligand in a sample, which comprises incubating, simultaneously or in sequence, (a) the sample (containing the analyte ligand), (b) a directly labelled specific binding partner to the ligand, (c) a ligand analogue as herein defined, conjugated with a reagent X (the said reagent not being present as a free reagent in the mixture); separating, after a suitable incubation period, the portion containing component (c) from the mixture by means of a solid phase carrying a binding partner specific for reagent X; and determining the extent of complexing between components (b) and (c) by measurement of the label.
Claim 3
Dependent Claim
14 words
A method as claimed in claim 1 wherein the analyte ligand is a hapten.
Claim 4
Dependent Claim
18 words
A method as claimed in claim 2 wherein the analyte ligand is a non-peptide hormone or metabolite thereof.
Claim 5
Independent Claim
19 words
A method as claimed in any one of claims 1 to 3 wherein component (b) is a directly-labelled antibody.
Claim 6
Dependent Claim
15 words
A method as claimed in claim 4 wherein component (b) is a directly-labelled monoclonal antibody.
Claim 7
Independent Claim
28 words
A method as claimed in any one of claims 1 to 3 wherein component (b) is a directly-labelled monoclonal or polyclonal antibody fraqment devoid of the Fc portion.
Claim 8
Independent Claim
26 words
A method as claimed in any one of claims 1 to 6 wherein reagent X is fluorescein isothiocyanate, rhodamine isothiocyanate, 2,4-dinitrofluorobenzene, phenyl isothiocyanate or dansyl chloride.
Claim 9
Independent Claim
27 words
A method as claimed in any one of claims 1 to 7 wherein reagent X and the ligand analogue in component (c) are separated by a spacer.
Claim 10
Dependent Claim
20 words
A method as claimed in claim 8 wherein the spacer is selected from proteins, peptides, oligosaccharides, polysaccharides and synthetic polymers.
Claim 11
Independent Claim
19 words
A method as claimed in any one of claims 1 to 9 wherein the solid phase comprises magnetisable particles.
Claim 12
Dependent Claim
34 words
A kit of reagents for carrying out a method of assay as claimed in claim 1 which comprises components (b) and (c) and a solid phase carrying a binding partner specific for reagent X.
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