EP0028132A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Method of passively adsorbing immuno-reactive haptens to solid phases
Assignee: Dynasciences Corp
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Filing: 1980-10-23
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Granted: —
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Expires: 2000-10-23
Abstract
Abstract The method comprises covalently binding an immuno-reactive to a selected macromolecular carrier and then contacting the resulting hapten-carrier conjugate at a selected concentration in a liquid phase with a selected solid phase until a desired amount of the hapten-carrier conjugate is adsorbed to the surface of the solid phase. Unbound hapten-carrier conjugate is then separated from the solid phase, and the solid phase containing the bound hapten-carrier conjugate is recovered for use in quantitative immunoassays and the like. The solid phase can be, for example, surfaces of a test tube or microtiter well or the like. The method is simple and inexpensive and permits hapten assays of improved sensitivity.
Inventors
- 👤 Harold R. Cooper
- 👤 Andrew O'beirne
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-03 09:58
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; G01N33/74; G01N33/78
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
77 words
A method of passively absorbing immuno-reactive hapten to a solid phase for an immuno-assay, which method comprises: a) covalently binding an immuno-reactive hapten to a selected macromolecular carrier; b) contacting the resulting hapten-carrier conjugate with a selected solid phase until a desired concentration of said hapten-carrier conjugate is absorbed to the surface of said solid phase; and, c) thereafter removing unbound hapten-carrier conjugate from said solid phase and recovering said solid phase containing said bound hapten-carrier conjugate.
Claim 2
Independent Claim
77 words
A method of passively absorbing immuno-reactive hapten to a solid phase for an immuno-assay, which method comprises: a) covalently binding an immuno-reactive hapten to a selected macromolecular carrier; b) contacting the resulting hapten-carrier conjugate with a selected solid phase until a desired concentration of said hapten-carrier conjugate is absorbed to the surface of said solid phase; and, c) thereafter removing unbound hapten-carrier conjugate from said solid phase and recovering said solid phase containing said bound hapten-carrier conjugate.
Claim 3
Dependent Claim
26 words
The method of claim 1 wherein said covalent binding of said hapten to said macromolecular carrier is effected through the use of a selected coupling agent.
Claim 4
Dependent Claim
23 words
The method of claim 2 wherein said hapten-carrier conjugate is in a liquid phase when said contacting with said solid phase is initiated.
Claim 5
Dependent Claim
31 words
"The method of claim 3 wherein said hapten-carrier conjugate is diluted to a desired concentration with a liquid diluent and then contacted with said solid phase while still in said diluent.
Claim 6
Dependent Claim
34 words
The method of claim 2 wherein said hapten comprises digoxin, wherein said macromolecular carrier comprises human serum albumin, wherein said coupling reagent comprises periodate and sodium borohydride, and wherein said solid phase comprises polystyrene.
Claim 7
Dependent Claim
31 words
The method of claim 2 wherein said hapten comprises thyroxin, wherein said macromolecular carrier comprises bovine serum albumin, wherein said coupling reagent comprises glutaraldehyde, and wherein said solid phase comprises polystyrene.
Claim 8
Dependent Claim
31 words
The method of claim 2 wherein said hapten comprises gentamicin, wherein said macromolecular carrier comprises bovine serum albumin, wherein said coupling reagent comprises glutaraldehyde, and wherein said solid phase comprises polystyrene.
Claim 9
Dependent Claim
32 words
The method of claim 2 wherein said hapten comprises triiodothyronine, wherein said macromolecular car=ier comprises bovine serum albumin, wherein said coupling reagent comprises glutaraldehyde, and wherein said solid phase comprises polyvinyl acetate.
References Cited (Backward Citations)
No citation records indexed.
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