US6835938B2 — Biopolymer array substrate thickness dependent au…

Claim 1

Independent Claim 109 words

A method for determining a biopolymer array substrate thickness dependent optimal focus distance of a biopolymer array scanner, the method comprising: (a) scanning portions of a reference substrate over a range of positions, each position representing a different distance between an illuminated region of the reference substrate and an emitted-light detection component of the scanner, each portion scanned at a different position; (b) assembling data collected from scanning the reference substrate into a representation of an intensity/position function; and (c) employing an array substrate thickness dependent focus-distance determination method to determine an array substrate thickness dependent optimal focus distance or range of focus distances for said biopolymer array scanner.

Claim 2

Independent Claim 109 words

A method for determining a biopolymer array substrate thickness dependent optimal focus distance of a biopolymer array scanner, the method comprising: (a) scanning portions of a reference substrate over a range of positions, each position representing a different distance between an illuminated region of the reference substrate and an emitted-light detection component of the scanner, each portion scanned at a different position; (b) assembling data collected from scanning the reference substrate into a representation of an intensity/position function; and (c) employing an array substrate thickness dependent focus-distance determination method to determine an array substrate thickness dependent optimal focus distance or range of focus distances for said biopolymer array scanner.

Claim 3

Dependent Claim 35 words

The method according to claim 1 , wherein said method further comprises determining the thickness of said reference substrate to obtain a substrate thickness value employed in said array substrate thickness dependent focus-distance determination method.

Claim 4

Dependent Claim 27 words

The method according to claim 2 , wherein the thickness of said reference substrate is determined using a position sensing detector (PSD) of said biopolymer array scanner.

Claim 5

Dependent Claim 39 words

The method according to claim 1 , wherein said method comprises determining an array substrate thickness dependent optimal focus distance or range of focus distances for said biopolymer array scanner for two or more reference substrates of different thickness.

Claim 6

Dependent Claim 52 words

The method according to claim 4 , wherein said method further comprises determining a calibration function of optimal focus distance versus substrate thickness from said determined array substrate thickness dependent optimal focus distances or ranges of focus distances for said biopolymer array scanner for two or more reference substrates of different thickness.

Claim 7

Dependent Claim 27 words

The method according to claim 5 , wherein said calibration function is stored in a non-volatile memory storage element of said scanner to produce a calibrated scanner.

Claim 8

Dependent Claim 54 words

A calibration function prepared according to the method of claim 5 encoded by one of: (a) storing said calibration function in a machine readable medium; (b) transmitting said calibration function over an electronic communications medium; (c) displaying said calibration function on display device; and (d) printing said calibration function in a human readable medium.

Claim 9

Dependent Claim 61 words

The method according to claim 1 , wherein scanning portions of a reference substrate over a range of positions relative to an emitted-light detection component further includes: for each position, scanning a set of rows, filtering the intensity values scanned for each row, and averaging the intensity values for the set of rows into an average intensity value for the position.

Claim 10

Dependent Claim 33 words

The method according to claim 1 , wherein assembling data collected from scanning the reference substrate into a representation of an intensity/position function further includes associating each position with an average intensity value.

Claim 11

Dependent Claim 53 words

The method according to claim 1 , wherein employing an array substrate thickness dependent focus-distance determination method to determine an optimal thickness dependent focus distance or range of focus distances comprises employing a method chosen from: (i) a peak-intensity-focus-distance determination method; (ii) a window-focus-distance determination method; and (iii) an average peak-intensity-focus-distance determination method.

Claim 12

Dependent Claim 43 words

The method according to claim 10 , wherein when said array substrate thickness is between about 0.95 and 1.05 mm, said employed array substrate thickness dependent focus-distance determination method is chosen from: (i) a peak-intensity-focus-distance determination method; and (ii) a window-focus-distance determination method.

Claim 13

Dependent Claim 22 words

The method according to claim 11 , wherein said employed array substrate thickness dependent focus-distance determination method is a peak-intensity-focus-distance determination method.

Claim 14

Dependent Claim 22 words

The method according to claim 11 , wherein said employed array substrate thickness dependent focus-distance determination method is a window-focus-distance determination method.

Claim 15

Dependent Claim 49 words

The method according to claim 10 , wherein when said array substrate thickness is less than about 0.95 mm or greater than about 1.05 mm, said employed array substrate thickness dependent focus-distance determination method is chosen from: (i) a peak-intensity-focus distance determination method; and (ii) an average-peak-intensity-focus-distance determination method.

Claim 16

Dependent Claim 24 words

The method according to claim 14 , wherein said employed array substrate thickness dependent focus distance determination method is a peak-intensity-focus distance determination method.

Claim 17

Dependent Claim 22 words

The method according to claim 14 , wherein said employed array substrate thickness dependent focus-distance determination method is an average-peak-intensity-focus-distance determination method.

Claim 18

Dependent Claim 75 words

The method according to claim 14 , wherein said average peak-intensity-focus-distance determination method comprises: (a) determining the position of the peak intensity in the intensity/position function; (b) identifying a left and right threshold position relative to said determined peak intensity position that have an intensity below a threshold value; and (c) determining the mean of said left and right threshold positions to determine said array substrate dependent optimal focus distance or range of focus distances.

Claim 19

Dependent Claim 45 words

A set of computer instructions for carrying out the method of claim 1 encoded by one of: storing the computer instructions in a machine readable medium; transmitting the computer instructions over an electronic communications medium; and printing the computer instructions in a human readable medium.

Claim 20

Independent Claim 46 words

A method of determining a biopolymer array substrate thickness, said method comprising: (a) positioning said array substrate on a receiving element of a biopolymer array scanner; and (b) employing a position sensing detector (PSD) of said biopolymer array scanner to determine said biopolymer array substrate thickness.

Claim 21

Independent Claim 46 words

A method of determining a biopolymer array substrate thickness, said method comprising: (a) positioning said array substrate on a receiving element of a biopolymer array scanner; and (b) employing a position sensing detector (PSD) of said biopolymer array scanner to determine said biopolymer array substrate thickness.

Claim 22

Dependent Claim 88 words

The method according to claim 19 , wherein said employing step (b) comprises: (i) identifying a first position of said receiving element wherein light reflected from a first surface of said array substrate is received at a predetermined location of said PSD; (ii) identifying a second position of said receiving element wherein light reflected from a second surface of said array is received at said predetermined location of said PSD; and (iii) determining the difference of said first and second positions to determine said biopolymer array substrate thickness.

Claim 23

Dependent Claim 75 words

A biopolymer array scanner comprising: (a) a probe-molecule excitation system; (b) an emitted-light photodetection system that produces a signal representative of the emitted-light intensity; (c) a biopolymer-array-holding stage that holds a biopolymer array for scanning and that can be moved through a range of positions that place an illuminated region of the surface of the biopolymer array at different distances from the emitted-light photodetection system; and (d) an automated array substrate thickness dependent focus-distance-determination subsystem.

Claim 24

Dependent Claim 75 words

A biopolymer array scanner comprising: (a) a probe-molecule excitation system; (b) an emitted-light photodetection system that produces a signal representative of the emitted-light intensity; (c) a biopolymer-array-holding stage that holds a biopolymer array for scanning and that can be moved through a range of positions that place an illuminated region of the surface of the biopolymer array at different distances from the emitted-light photodetection system; and (d) an automated array substrate thickness dependent focus-distance-determination subsystem.

Claim 25

Dependent Claim 116 words

The biopolymer array scanner according to claim 21 , wherein said automated array substrate thickness dependent focus-distance-determination subsystem is a set of computer instructions for performing a method comprising: (a) scanning portions of a reference substrate over a range of positions, each position representing a different distance between an illuminated region of the reference substrate and an emitted-light detection component of the scanner, each portion scanned at a different position; (b) assembling data collected from scanning the reference substrate into a representation of an intensity/position function; and employing an array substrate thickness dependent focus-distance determination method to determine an array substrate thickness dependent optimal focus distance or range of focus distances for said biopolymer array scanner.

Claim 26

Dependent Claim 83 words

A calibrated biopolymer array scanner comprising: (a) a probe-molecule excitation system; (b) an emitted-light photodetection system that produces a signal representative of the emitted-light intensity; (c) a biopolymer-array-holding stage that holds a biopolymer array for scanning and that can be moved through a range of positions that place an illuminated region of the surface of the biopolymer array at different distances from the emitted-light photodetection system; and (d) a calibration function that selects an optimal focus distance based on an array substrate thickness.

Claim 27

Dependent Claim 83 words

A calibrated biopolymer array scanner comprising: (a) a probe-molecule excitation system; (b) an emitted-light photodetection system that produces a signal representative of the emitted-light intensity; (c) a biopolymer-array-holding stage that holds a biopolymer array for scanning and that can be moved through a range of positions that place an illuminated region of the surface of the biopolymer array at different distances from the emitted-light photodetection system; and (d) a calibration function that selects an optimal focus distance based on an array substrate thickness.

Claim 28

Dependent Claim 22 words

The scanner according to claim 23 , wherein said calibration function is a calibration function of optimal focus distance versus substrate thickness.

Claim 29

Dependent Claim 51 words

A method of assaying a sample, said method comprising: (a) contacting said sample with a biopolymeric array of two or more biopolymer ligands immobilized on a surface of a solid support; and (b) reading said array with a calibrated biopolymer array optical scanner according to claim 23 to obtain a result.

Claim 30

Dependent Claim 36 words

The method according to claim 25 , wherein said reading step (b) includes: (i) determining the thickness of said biopolymeric array; and (ii) automatically selecting an optimal focus distance using said determined thickness and calibration function.

Claim 31

Dependent Claim 22 words

The method according to claim 26 , wherein said calibration function is a calibration function of optimal focus distance versus substrate thickness.

Claim 32

Independent Claim 56 words

A method of reading a biopolymer array, said method comprising: (a) determining said biopolymer array's substrate thickness; (b) automatically selecting an optimal focus distance using the determined substrate thickness and a calibration function on substrate thickness versus optimal focus distance; and (c) optically reading said biopolymer array to obtain a result using said optimal focus distance.

Claim 33

Independent Claim 56 words

A method of reading a biopolymer array, said method comprising: (a) determining said biopolymer array's substrate thickness; (b) automatically selecting an optimal focus distance using the determined substrate thickness and a calibration function on substrate thickness versus optimal focus distance; and (c) optically reading said biopolymer array to obtain a result using said optimal focus distance.

Claim 34

Dependent Claim 56 words

The method according to claim 28 , wherein said thickness is determined using the PSD based method according to a method comprising: (a) positioning said array substrate on a receiving element of a biopolymer array scanner; and (b) employing a position sensing detector (PSD) of said biopolymer array scanner to determine said biopolymer array substrate thickness.

Claim 35

Dependent Claim 22 words

The method according to claim 28 , wherein said biopolymer array is chosen from a polypeptide array and a nucleic acid array.

Claim 36

Dependent Claim 20 words

The method according to claim 28 , further comprising transmitting said result from a first location to a second location.

Claim 37

Dependent Claim 15 words

The method according to claim 31 , where said second location is a remote location.

Claim 38

Dependent Claim 19 words

A method comprising receiving data representing said result of a scan obtained by the method of claim 28 .

Claim 39

Dependent Claim 33 words

A kit for use in a biopolymer array optical scanner, said kit comprising: (a) a computer-readable medium according to claim 19 ; and (b) instructions for operating said scanner according to said programming.

Claim 40

Dependent Claim 17 words

The kit according to claim 34 , wherein said kit further includes at least one reference array.

Annuity Stage	Window Opens	Payment Deadline	Surcharge Period Until	Estimated Fee	Status
3.5 Years (Year 4)	2007-12-28	2008-06-28	2008-12-28	$2000.00	Window Closed / Lapsed
7.5 Years (Year 8)	2011-12-28	2012-06-28	2012-12-28	$3760.00	Window Closed / Lapsed
11.5 Years (Year 12)	2015-12-28	2016-06-28	2016-12-28	$7700.00	Window Closed / Lapsed

Biopolymer array substrate thickness dependent automated focus-distance determination method for biopolymer array scanners

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