US6552172B2
Expired / Lapsed
US
⏱️ 0.0 years remaining
Fibrin nanoparticles and uses thereof
Assignee: Hapto Biotech Inc
•
Filing: 2001-08-30
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Granted: 2003-04-22
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Expires: 2021-08-30
Abstract
Abstract The present invention provides fibrin nanoparticles having a mean diameter of 200-2000 nm. The present invention provides also methods for preparing fibrin particles of various sizes, including fibrin nanoparticles and fibrin microbeads. The present invention further provides compositions comprising fibrin particles and an agent, wherein the agent is coupled to the amine or carboxy moieties on the surface of the fibrin particles. In addition the present invention provides a composition comprising fibrin nanoparticles and an agent, wherein the agent is admixed with the fibrin nanoparticles. Still further, the present invention provides a method for introducing an agent into a cell, a method for isolating stem and/or progenitor cells from a biological sample, as well as a composition comprising fibrin particles bound to stem and/or progenitor cells.
Inventors
- 👤 Gerard Marx
- 👤 Raphael Gorodetsky
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:58
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/745; C07K14/75; B; B82; B82Y; B82Y5/00; Y; Y10; Y10S; Y10S977/00; Y10S977/70; Y10S977/773; Y10S977/902; Y10S977/904; Y10S977/908; Y10S977/91
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
10 words
Isolated fibrin nanoparticles having a mean diameter of 200-2000 nm.
Claim 2
Dependent Claim
10 words
Isolated fibrin nanoparticles having a mean diameter of 200-2000 nm.
Claim 3
Dependent Claim
13 words
The fibrin nanoparticles of claim 1 , having a density of 1.32±0.2 g/ml.
Claim 4
Independent Claim
81 words
A method for preparing fibrin nanoparticles comprising the steps of: (i) mixing an aqueous solution comprising fibrinogen, thrombin and Factor XIII in an oil emulsion at a temperature of 50-80° C., without the addition of an exogenous chemical cross-linking agent, until fibrin microbeads are formed in the mixture; (ii) homogenizing the mixture to form fibrin nanoparticles having a mean diameter of 200-2000 nm in the mixture; and (iii) isolating fibrin nanoparticles having a mean diameter of 200-2000 nm from the mixture.
Claim 5
Independent Claim
81 words
A method for preparing fibrin nanoparticles comprising the steps of: (i) mixing an aqueous solution comprising fibrinogen, thrombin and Factor XIII in an oil emulsion at a temperature of 50-80° C., without the addition of an exogenous chemical cross-linking agent, until fibrin microbeads are formed in the mixture; (ii) homogenizing the mixture to form fibrin nanoparticles having a mean diameter of 200-2000 nm in the mixture; and (iii) isolating fibrin nanoparticles having a mean diameter of 200-2000 nm from the mixture.
Claim 6
Dependent Claim
28 words
The method of claim 3 , wherein the aqueous solution comprising fibrinogen, thrombin and Factor XIII is prepared by contacting thrombin with purified fibrinogen containing endogenous Factor XIII.
Claim 7
Dependent Claim
31 words
The method of claim 3 , wherein the aqueous solution comprising fibrinogen, thrombin and Factor XIII is prepared by combining thrombin with a cryoprecipitate containing endogenous fibrinogen and endogenous Factor XIII.
Claim 8
Dependent Claim
28 words
The method of claim 3 , wherein the aqueous solution comprising fibrinogen, thrombin and Factor XIII is prepared by adding separate amounts of fibrinogen, Factor XIII and thrombin.
Claim 9
Dependent Claim
20 words
The method of claim 3 , wherein ratio of fibrinogen:thrombin:Factor XIII in the aqueous solution is 5-100 mg/mL:1-100 U/mL:1-50 U/mL.
Claim 10
Dependent Claim
20 words
The method of claim 3 , wherein ratio of fibrinogen:thrombin:Factor XIII in the aqueous solution is 20-40 mg/mL:5-10 U/mL:2-20 U/mL.
Claim 11
Dependent Claim
16 words
The method of claim 3 , wherein the aqueous solution further comprises at least one agent.
Claim 12
Dependent Claim
26 words
The method of claim 9 , wherein the agent is a drug, protein, peptide, nucleic acid, saccharide, antigen, hapten, alginate, biodegradable polymer, polyethylene glycol or sugar.
Claim 13
Dependent Claim
30 words
The method of claim 3 , wherein the aqueous solution comprising fibrinogen, Factor XIII and thrombin is introduced into the oil within about 30 seconds after preparing the aqueous solution.
Claim 14
Dependent Claim
33 words
The method of claim 3 , wherein the oil in the oil emulsion is selected from the group consisting of corn oil, olive oil, soy oil, castor oil, mineral oil and combinations thereof.
Claim 15
Dependent Claim
12 words
The method of claim 12 , wherein the oil is corn oil.
Claim 16
Dependent Claim
18 words
The method of claim 3 , wherein the mixture in step (i) is mixed for about 3-9 hours.
Claim 17
Dependent Claim
23 words
The method of claim 3 , wherein the fibrin nanoparticles having a mean diameter of 200-2000 nm are isolated by filtration and sedimentation.
Claim 18
Dependent Claim
20 words
The method of claim 3 , wherein the fibrin nanoparticles isolated in step (iii) have a density of 1.32±0.2 g/mL.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 2006-04-21 | 2006-10-21 | 2007-04-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2010-04-21 | 2010-10-21 | 2011-04-22 | $3760.00 | Window Closed / Lapsed |
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