US5324647A
Expired / Lapsed
US
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Fibrin coated polymer surfaces
Assignee: McMaster University
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Filing: 1993-09-14
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Granted: 1994-06-28
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Expires: 2013-09-14
Abstract
Abstract Surfaces of polymeric materials are coated with a layer of thermally denatured fibrinogen. The fibrinogen may be treated with thrombin to produce fibrin, and additional fibrinogen and factor XIII added to produce a cross-linked fibrin coating. The resulting coated surface is stable, anti-thrombotic and resistant to platelet adhesion. Polymeric materials coated in this way are useful in constructing vascular grafts and prosthetic devices, and provide test systems useful in the study of thrombogenesis.
Inventors
- 👤 Fraser D. Rubens
- 👤 Paul D. Bishop
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 03:03
CPC Classifications
A; A61; A61L; A61L33/00; A61L33/06; A61L33/12; A61L33/126; A61L27/00; A61L27/28; A61L27/34; Y; Y10; Y10T; Y10T436/00; Y10T436/10
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
82 words
In a method of coating a surface of a polymeric material with fibrin, the improvement comprising: incubating said polymeric material in a solution of fibrinogen at a temperature of at least 56° C. but less than 100° C. to produce a fibrinogen-coated surface; treating the fibrinogen-coated surface with thrombin to produce fibrin monomers; and then exposing the thrombin-treated surface to a solution comprising factor XIII and additional fibrinogen, whereby the additional fibrinogen is converted to fibrin and cross-linked to the fibrin-coated surface.
Claim 2
Dependent Claim
82 words
In a method of coating a surface of a polymeric material with fibrin, the improvement comprising: incubating said polymeric material in a solution of fibrinogen at a temperature of at least 56° C. but less than 100° C. to produce a fibrinogen-coated surface; treating the fibrinogen-coated surface with thrombin to produce fibrin monomers; and then exposing the thrombin-treated surface to a solution comprising factor XIII and additional fibrinogen, whereby the additional fibrinogen is converted to fibrin and cross-linked to the fibrin-coated surface.
Claim 3
Dependent Claim
24 words
A method according to claim 1 wherein the factor XIII concentration in the solution comprising factor XIII and additional fibrinogen is from 0.25-20 μg/mL.
Claim 4
Dependent Claim
24 words
A method according to claim 1 wherein the factor XIII concentration in the solution comprising factor XIII and additional fibrinogen is from 0.25-5.0 μg/mL.
Claim 5
Dependent Claim
23 words
A method according to claim 1 wherein the fibrinogen concentration in the solution comprising factor XIII and additional fibrinogen is from 0.5-5.0 mg/mL.
Claim 6
Dependent Claim
36 words
A method according to claim 1 wherein said solution comprising factor XIII and additional fibrinogen further comprises basic fibroblast growth factor, endothelial cell growth factor, α 2 macroglobulin, vitronectin, fibronectin, or a cell-binding fragment of fibronectin.
Claim 7
Dependent Claim
18 words
A method according to claim 1 wherein said solution comprising factor XIII and additional fibrinogen comprises endothelial cells.
Claim 8
Dependent Claim
18 words
A method according to claim 1 further comprising the step of seeding the cross-linked fibrin with endothelial cells.
Claim 9
Dependent Claim
11 words
A polymeric material prepared according to the method of claim 1.
Claim 10
Dependent Claim
36 words
A polymeric material prepared according to the method of incubating said polymeric material in a solution of fibrinogen at a temperature of at least 56° C. but less than 100° C. to produce a fibrinogen-coated surface.
Claim 11
Dependent Claim
36 words
A polymeric material prepared according to the method of incubating said polymeric material in a solution of fibrinogen at a temperature of at least 56° C. but less than 100° C. to produce a fibrinogen-coated surface.
Claim 12
Dependent Claim
48 words
A polymeric material prepared according to the method of: incubating said polymeric material in a solution of fibrinogen at a temperature of at least 56° C. but less than 100° C. to produce a fibrinogen-coated surface; and then treating the fibrinogen-coated surface with thrombin to produce fibrinogen monomers.
Claim 13
Dependent Claim
48 words
A polymeric material prepared according to the method of: incubating said polymeric material in a solution of fibrinogen at a temperature of at least 56° C. but less than 100° C. to produce a fibrinogen-coated surface; and then treating the fibrinogen-coated surface with thrombin to produce fibrinogen monomers.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-06-27 | 1997-12-27 | 1998-06-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-06-27 | 2001-12-27 | 2002-06-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-06-27 | 2005-12-27 | 2006-06-28 | $7700.00 | Window Closed / Lapsed |