Patent US6506559B1 successfully refreshed from Google Patents.
US6506559B1
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Genetic inhibition by double-stranded RNA
Assignee: Carnegie Institution for Science
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Filing: 1998-12-18
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Granted: 2003-01-14
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Expires: 2018-12-18
Abstract
Abstract A process is provided of introducing an RNA into a living cell to inhibit gene expression of a target gene in that cell. The process may be practiced ex vivo or in vivo. The RNA has a region with double-stranded structure. Inhibition is sequence-specific in that the nucleotide sequences of the duplex region of the RNA and of a portion of the target gene are identical. The present invention is distinguished from prior art interference in gene expression by antisense or triple-strand methods.
Inventors
- 👤 Andrew Fire
- 👤 Stephen Kostas
- 👤 Mary Montgomery
- 👤 Lisa Timmons
- 👤 SiQun Xu
- 👤 Hiroaki Tabara
- 👤 Samuel E. Driver
- 👤 Craig C. Mello
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 11:48
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8241; C12N15/8261; C12N15/8271; C12N15/8279; C12N15/8285; A; A61; A61K; A61K31/00; A61K31/70; A61K31/7088; A61K31/7105; A61K31/713; A61P; A61P35/00; A61P35/02; A61P43/00; C12N15/11; C12N15/113; C12N15/8216; C12N15/8218; C12N15/85; A01; A01K; A01K2217/00; A01K2217/05; A01K2227/00; A01K2227/70; A01K2227/703; C12N2310/00; C12N2310/10; C12N2310/11; C12N2310/14; C12N2310/30; C12N2310/32; C12N2310/321; C12N2310/50; C12N2310/53
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
115 words
A method to inhibit expression of a target gene in a cell in vitro comprising introduction of a ribonucleic acid (RNA) into the cell in an amount sufficient to inhibit expression of the target gene, wherein the RNA is a double-stranded molecule with a first strand consisting essentially of a ribonucleotide sequence which corresponds to a nucleotide sequence of the target gene and a second strand consisting essentially of a ribonucleotide sequence which is complementary to the nucleotide sequence of the target gene, wherein the first and the second ribonucleotide strands are separate complementary strands that hybridize to each other to form said double-stranded molecule, and the double-stranded molecule inhibits expression of the target gene.
Claim 2
Independent Claim
115 words
A method to inhibit expression of a target gene in a cell in vitro comprising introduction of a ribonucleic acid (RNA) into the cell in an amount sufficient to inhibit expression of the target gene, wherein the RNA is a double-stranded molecule with a first strand consisting essentially of a ribonucleotide sequence which corresponds to a nucleotide sequence of the target gene and a second strand consisting essentially of a ribonucleotide sequence which is complementary to the nucleotide sequence of the target gene, wherein the first and the second ribonucleotide strands are separate complementary strands that hybridize to each other to form said double-stranded molecule, and the double-stranded molecule inhibits expression of the target gene.
Claim 3
Dependent Claim
14 words
The method of claim 1 in which the target gene is a cellular gene.
Claim 4
Dependent Claim
14 words
The method of claim 1 in which the target gene is an endogenous gene.
Claim 5
Dependent Claim
13 words
The method of claim 1 in which the target gene is a transgene.
Claim 6
Dependent Claim
14 words
The method of claim 1 in which the target gene is a viral gene.
Claim 7
Dependent Claim
13 words
The method of claim 1 in which the cell is from an animal.
Claim 8
Dependent Claim
13 words
The method of claim 1 in which the cell is from a plant.
Claim 9
Dependent Claim
14 words
The method of claim 6 in which the cell is from an invertebrate animal.
Claim 10
Dependent Claim
13 words
The method of claim 8 in which the cell is from a nematode.
Claim 11
Dependent Claim
43 words
The method of claim 1 in which the first ribonucleotide sequence comprises at least 25 bases which correspond to the target gene and the second ribonucleotide sequence comprises at least 25 bases which are complementary to the nucleotide sequence of the target gene.
Claim 12
Dependent Claim
17 words
The method of claim 1 in which the target gene expression is inhibited by at least 10%.
Claim 13
Independent Claim
146 words
A method to inhibit expression of a target gene in an invertebrate organism comprising: (a) providing an invertebrate organism containing a target cell, wherein the target cell contains the target gene and the target cell is susceptible to RNA interference, and the target gene is expressed in the target cell; (b) contacting said invertebrate organism with a ribonucleic acid (RNA), wherein the RNA is a double-stranded molecule with a first strand consisting essentially of a ribonucleotide sequence which corresponds to a nucleotide sequence of the target gene and a second strand consisting essentially of a ribonucleotide sequence which is complementary to the nucleotide sequence of the target gene, wherein the first and the second ribonucleotide sequences are separate complementary strands that hybridize to each other to form the double-stranded molecule; and (c) introducing the RNA into the target cell, thereby inhibiting expression of the target gene.
Claim 14
Independent Claim
146 words
A method to inhibit expression of a target gene in an invertebrate organism comprising: (a) providing an invertebrate organism containing a target cell, wherein the target cell contains the target gene and the target cell is susceptible to RNA interference, and the target gene is expressed in the target cell; (b) contacting said invertebrate organism with a ribonucleic acid (RNA), wherein the RNA is a double-stranded molecule with a first strand consisting essentially of a ribonucleotide sequence which corresponds to a nucleotide sequence of the target gene and a second strand consisting essentially of a ribonucleotide sequence which is complementary to the nucleotide sequence of the target gene, wherein the first and the second ribonucleotide sequences are separate complementary strands that hybridize to each other to form the double-stranded molecule; and (c) introducing the RNA into the target cell, thereby inhibiting expression of the target gene.
Claim 15
Dependent Claim
12 words
The method of claim 12 in which the organism is a nematode.
Claim 16
Dependent Claim
33 words
The method of claim 13 in which a formulation comprised of the RNA is applied on or adjacent to a plant, and disease associated with nematode infection of the plant is thereby reduced.
Claim 17
Dependent Claim
46 words
The method of claim 12 in which said double-stranded ribonucleic acid structure is at least 25 bases in length and each of the ribonucleic acid strands is able to specifically hybridize to a deoxyribonucleic acid strand of the target gene over the at least 25 bases.
Claim 18
Dependent Claim
19 words
The method of claim 12 in which the expression of the target gene is inhibited by at least 10%.
Claim 19
Dependent Claim
23 words
The method of claim 12 in which the RNA is introduced within a body cavity of the organism and outside the target cell.
Claim 20
Dependent Claim
17 words
The method of claim 12 in which the RNA is introduced by extracellular injection into the organism.
Claim 21
Dependent Claim
22 words
The method of claim 12 in which the organism is contacted with the RNA by feeding the organism food containing the RNA.
Claim 22
Dependent Claim
16 words
The method of claim 19 in which the food comprises a genetically-engineered host transcribing the RNA.
Claim 23
Dependent Claim
22 words
The method of claim 12 in which at least one strand of the RNA is produced by transcription of an expression construct.
Claim 24
Dependent Claim
33 words
The method of claim 21 in which the organism is a nematode and the expression construct is contained in a plant, and disease associated with nematode infection of the plant is thereby reduced.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 2006-01-13 | 2006-07-15 | 2007-01-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2010-01-13 | 2010-07-15 | 2011-01-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2014-01-13 | 2014-07-15 | 2015-01-14 | $7700.00 | Window Closed / Lapsed |