US5874555A
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US
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Triple helices and processes for making same
Assignee: California Institute of Technology
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Filing: 1993-11-22
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Granted: 1999-02-23
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Expires: 2013-11-22
Abstract
Abstract Oligonucleotides and processes for their use for specific recognition of a target sequence in double-stranded nucleic acid through the formation of a triple helix. The oligonucleotides can bind in a parallel or antiparallel orientation to one of the strands of the target sequence depending upon the sequence composition of the target sequence and oligonucleotide used. The oligonucleotides are used as diagnostic or therapeutic agents through incorporation of an appropriate moiety in one or more nucleotides in the triple helix forming oligonucleotide.
Inventors
- 👤 Peter B. Dervan
- 👤 Heinz E. Moser
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 21:38
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6839
Claims Analysis
Total Claims: 38
Claim 1
Independent Claim
70 words
A triple helix comprising a double-helical nucleic acid having more than 500 nucleotide base pairs and comprising first and second strands and a heteropolymeric oligonucleotide bound to a target sequence within said double-helical nucleic acid, wherein when said oligonucleotide is bound in a parallel orientation to said first strand, said oligonucleotide comprises a T or a C + bound to an A or a G respectively, on said first strand.
Claim 2
Dependent Claim
70 words
A triple helix comprising a double-helical nucleic acid having more than 500 nucleotide base pairs and comprising first and second strands and a heteropolymeric oligonucleotide bound to a target sequence within said double-helical nucleic acid, wherein when said oligonucleotide is bound in a parallel orientation to said first strand, said oligonucleotide comprises a T or a C + bound to an A or a G respectively, on said first strand.
Claim 3
Dependent Claim
31 words
The triple helix of claim 1 wherein said oligonucleotide comprises a pyrimidine-rich oligonucleotide and said target sequence comprises a purine-rich sequence on one of the strands of said double-helical nucleic acid.
Claim 4
Dependent Claim
24 words
The triple helix of claim 2 wherein said pyrimidine-rich oligonucleotide binds to said target sequence in a parallel orientation to said purine-rich target sequence.
Claim 5
Dependent Claim
19 words
The triple helix of claim 1 wherein said oligonucleotide is bound in said parallel orientation to said first strand.
Claim 6
Dependent Claim
31 words
The triple helix of claim 1 wherein said oligonucleotide comprises a purine-rich oligonucleotide and said target sequence comprises a purine-rich sequence on one of the strands of said double-helical nucleic acid.
Claim 7
Dependent Claim
24 words
The triple helix of claim 5 wherein said purine-rich oligonucleotide binds to said target sequence in an antiparallel orientation to said purine-rich target sequence.
Claim 8
Dependent Claim
44 words
The triple helix of claim 6 wherein said purine-rich oligonucleotide contains a G when the nucleotide at the complementary position in said purine-rich target sequence is G and an A when the nucleotide at the complementary position in said purine-rich target sequence is A.
Claim 9
Dependent Claim
47 words
The triple helix of claim 7 wherein said purine-rich target sequence contains up to about 40% of the pyrimidine nucleotide C and said purine-rich oligonucleotide contains the pyrimidine nucleotide T when the nucleotide at the nucleotide at the complementary position in said purine-rich target sequence is C.
Claim 10
Dependent Claim
17 words
The triple helix of claim 8 wherein said purine-rich target sequence contains up to about 25% C.
Claim 11
Dependent Claim
79 words
The triple helix of claim 6 wherein said purine-rich target sequence contains one or more or the pyrimidine nucleotide C and said purine-rich oligonucleotide contains a G when the nucleotide at the complementary position in said purine-rich target sequence is G, an A or a T when the nucleotide at the complementary position in said purine-rich target sequence is an A and a nebularine nucleotide when the nucleotide at the complementary position in said purine-rich target sequence is C.
Claim 12
Dependent Claim
31 words
The triple helix of claim 1 wherein said oligonucleotide bound to said target sequence within said double-helical nucleic acid further comprises at least one nucleotide to which a moiety is attached.
Claim 13
Dependent Claim
13 words
The triple helix of claim 11 wherein said moiety is a detectable moiety.
Claim 14
Dependent Claim
22 words
The triple helix of claim 11 wherein said moiety is a cleaving moiety capable of causing cleavage of said double-helical nucleic acid.
Claim 15
Dependent Claim
16 words
The triple helix of claim 1 wherein said double-helical nucleic acid is an isolated nucleic acid.
Claim 16
Dependent Claim
14 words
The triple helix of claim 1 wherein said double-helical nucleic acid is substantially pure.
Claim 17
Dependent Claim
22 words
The triple helix of claim 1 wherein said target sequence comprises at least one purine nucleotide and at least one pyrimidine nucleotide.
Claim 18
Dependent Claim
16 words
The triple helix of claim 1 wherein said oligonucleotide is at least eleven nucleotides in length.
Claim 19
Dependent Claim
16 words
The triple helix of claim 1 wherein said oligonucleotide is at least fifteen nucleotides in length.
Claim 20
Dependent Claim
15 words
The triple helix of claim 1 wherein said oligonucleotide at least eighteen nucleotides in length.
Claim 21
Dependent Claim
93 words
A process for forming a triple helix wherein a heteropolymeric oligonucleotide is bound to a target sequence within a double-helical nucleic acid having more than 500 nucleotide base pairs and comprising first and second strands, comprising contacting said double-helical nucleic acid with a heteropolymeric oligonucleotide capable of binding to a target sequence contained within said double-helical nucleic acid, wherein when said oligonucleotide is bound in a parallel orientation to said first strand, said oligonucleotide comprises a T or a C + bound to an A or a G respectively, on said first strand.
Claim 22
Dependent Claim
93 words
A process for forming a triple helix wherein a heteropolymeric oligonucleotide is bound to a target sequence within a double-helical nucleic acid having more than 500 nucleotide base pairs and comprising first and second strands, comprising contacting said double-helical nucleic acid with a heteropolymeric oligonucleotide capable of binding to a target sequence contained within said double-helical nucleic acid, wherein when said oligonucleotide is bound in a parallel orientation to said first strand, said oligonucleotide comprises a T or a C + bound to an A or a G respectively, on said first strand.
Claim 23
Dependent Claim
29 words
The method claim 20 wherein said oligonucleotide comprises a pyrimidine-rich oligonucleotide and said target sequence comprises a purine-rich sequence on one of the strands of said double-helical nucleic acid.
Claim 24
Dependent Claim
23 words
The method of claim 21 wherein said pyrimidine-rich oligonucleotide binds to said target sequence in a parallel orientation to said purine-rich target sequence.
Claim 25
Dependent Claim
18 words
The method of claim 20 wherein said oligonucleotide is bound in said parallel orientation to said first strand.
Claim 26
Dependent Claim
30 words
The method of claim 20 wherein said oligonucleotide comprises a purine-rich oligonucleotide and said target sequence comprises a purine-rich sequence on one of the strands of said double-helical nucleic acid.
Claim 27
Dependent Claim
23 words
The method of claim 24 wherein said purine-rich oligonucleotide binds to said target sequence in an antiparallel orientation to said purine-rich target sequence.
Claim 28
Dependent Claim
44 words
The method of claim 25 wherein said purine-rich oligonucleotide contains a G when the nucleotide at the complementary position in said purine-rich target sequence is G and an A when the nucleotide at the complementary position in said purine-rich target sequence is an A.
Claim 29
Dependent Claim
46 words
The method of claim 26 wherein said purine-rich target sequence contains up to about 40% of the pyrimidine nucleotide C and said purine-rich oligonucleotide contains the pyrimidine nucleotide T when the nucleotide at the nucleotide at the complementary position in said purine-rich target sequence is C.
Claim 30
Dependent Claim
16 words
The method of claim 27 wherein said purine-rich target sequence contains up to about 25% C.
Claim 31
Dependent Claim
78 words
The method of claim 25 wherein said purine-rich target sequence contains one or more or the pyrimidine nucleotide C and said purine-rich oligonucleotide contains a G when the nucleotide at the complementary position in said purine-rich target sequence is G, an A or a T when the nucleotide at the complementary position in said purine-rich target sequence is an A and a nebularine nucleotide when the nucleotide at the complementary position in said purine-rich target sequence is C.
Claim 32
Dependent Claim
30 words
The method of claim 20 wherein said oligonucleotide bound to said target sequence within said double-helical nucleic acid further comprises at least one nucleotide to which a moiety is attached.
Claim 33
Dependent Claim
12 words
The method of claim 30 wherein said moiety is a detectable moiety.
Claim 34
Dependent Claim
21 words
The method of claim 30 wherein said moiety is a cleaving moiety capable of causing cleavage of said double-helical nucleic acid.
Claim 35
Dependent Claim
15 words
The process of claim 20 wherein said double-helical nucleic acid is an isolated nucleic acid.
Claim 36
Dependent Claim
13 words
The process of claim 20 wherein said double-helical nucleic acid is substantially pure.
Claim 37
Dependent Claim
41 words
A process for forming a triple-helix comprising contacting a triple-helical nucleic acid having more than 500 nucleotide base pairs with an oligonucleotide comprising a nebularine-containing nucleotide which is capable of binding to a target sequence contained within said double-helical nucleic acid.
Claim 38
Dependent Claim
41 words
A process for forming a triple-helix comprising contacting a triple-helical nucleic acid having more than 500 nucleotide base pairs with an oligonucleotide comprising a nebularine-containing nucleotide which is capable of binding to a target sequence contained within said double-helical nucleic acid.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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