US5190931A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA
Assignee: Research Foundation of the State University of New York
•
Filing: 1989-11-15
•
Granted: 1993-03-02
•
Expires: 2009-11-15
Abstract
Abstract Gene expression in a cell can be regulated or inhibited by incorporating into or associating with the genetic material of the cell a non-native nucleic acid sequence which is transcribed to produce an mRNA which is complementary to and capable of binding to the mRNA produced by the genetic material of said cell.
Inventors
- 👤 Masayori Inouye
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 00:03
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/113; C07; C07H; C07H21/00; A; A61; A61K; A61K38/00; C12N2310/00; C12N2310/10; C12N2310/11; C12N2310/111
Claims Analysis
Total Claims: 94
Claim 1
Independent Claim
88 words
A prokaryotic or eukaryotic cell containing a non-native DNA construct, which construct produces an RNA which regulates the function of a gene, said DNA construct containing the following operably linked DNA segments: a. a transcriptional promoter segment; b. a transcription termination segment; and therebetween c. a DNA segment; whereby transcription of the DNA segment produces a ribonucleotide sequence which does not naturally occur in the cell, is complementary to a ribonucleotide sequence transcribed from said gene, and said non-naturally occurring ribonucleotide sequence regulates the function of said gene.
Claim 2
Dependent Claim
88 words
A prokaryotic or eukaryotic cell containing a non-native DNA construct, which construct produces an RNA which regulates the function of a gene, said DNA construct containing the following operably linked DNA segments: a. a transcriptional promoter segment; b. a transcription termination segment; and therebetween c. a DNA segment; whereby transcription of the DNA segment produces a ribonucleotide sequence which does not naturally occur in the cell, is complementary to a ribonucleotide sequence transcribed from said gene, and said non-naturally occurring ribonucleotide sequence regulates the function of said gene.
Claim 3
Dependent Claim
95 words
A prokaryotic or eukaryotic cell containing a non-native DNA construct, which construct produces an RNA which regulates the function of a gene, said DNA construct containing the following operably linked DNA segments: a. a transcriptional promoter segment; b. a transcription termination segment; and c. a DNA segment comprising a segment of said gene, said gene segment located between said promoter segment and said termination segment and being inverted with respect to said promoter segment and said termination segment, whereby the RNA produced by transcription of the inverted gene segment regulates the function of said gene.
Claim 4
Dependent Claim
95 words
A prokaryotic or eukaryotic cell containing a non-native DNA construct, which construct produces an RNA which regulates the function of a gene, said DNA construct containing the following operably linked DNA segments: a. a transcriptional promoter segment; b. a transcription termination segment; and c. a DNA segment comprising a segment of said gene, said gene segment located between said promoter segment and said termination segment and being inverted with respect to said promoter segment and said termination segment, whereby the RNA produced by transcription of the inverted gene segment regulates the function of said gene.
Claim 5
Dependent Claim
27 words
A method of regulating the function of a gene in a prokaryotic or eukaryotic cell which comprises introducing into said cell the DNA construct of claim 1.
Claim 6
Dependent Claim
54 words
A method of regulating the function of a gene in a prokaryotic or eukaryotic cell which comprises: introducing into said cell the DNA construct of claim 1 whereby a transformed cell is obtained; and growing said transformed cell whereby the RNA produced by transcription of said DNA segment regulates the functioning of said gene.
Claim 7
Dependent Claim
99 words
A non-native DNA construct which, when present in a prokaryotic or eukaryotic cell containing a gene, produces an RNA which regulates the function of said gene, said DNA construct containing the following operably linked DNA segments: a. a transcriptional promoter segment; b. a transcription termination segment; and c. a DNA segment comprising a segment of said gene, said gene segment located between said promoter segment and said termination segment and being inverted with respect to said promoter segment and said termination segment, whereby the RNA produced by transcription of the inverted gene segment regulates the function of said gene.
Claim 8
Dependent Claim
99 words
A non-native DNA construct which, when present in a prokaryotic or eukaryotic cell containing a gene, produces an RNA which regulates the function of said gene, said DNA construct containing the following operably linked DNA segments: a. a transcriptional promoter segment; b. a transcription termination segment; and c. a DNA segment comprising a segment of said gene, said gene segment located between said promoter segment and said termination segment and being inverted with respect to said promoter segment and said termination segment, whereby the RNA produced by transcription of the inverted gene segment regulates the function of said gene.
Claim 9
Dependent Claim
92 words
A non-native DNA construct which, when present in a prokaryotic or eukaryotic cell containing a gene, produces an RNA which regulates the function of said gene, said DNA construct containing the following operably linked DNA segments: a. a transcriptional promoter segment; b. a transcription termination segment; and therebetween c. a DNA segment; whereby transcription of the DNA segment produces a ribonucleotide sequence which does not naturally occur in the cell, is complementary to a ribonucleotide sequence transcribed from said gene, and said non-naturally occurring ribonucleotide sequence regulates the function of said gene.
Claim 10
Dependent Claim
92 words
A non-native DNA construct which, when present in a prokaryotic or eukaryotic cell containing a gene, produces an RNA which regulates the function of said gene, said DNA construct containing the following operably linked DNA segments: a. a transcriptional promoter segment; b. a transcription termination segment; and therebetween c. a DNA segment; whereby transcription of the DNA segment produces a ribonucleotide sequence which does not naturally occur in the cell, is complementary to a ribonucleotide sequence transcribed from said gene, and said non-naturally occurring ribonucleotide sequence regulates the function of said gene.
Claim 11
Dependent Claim
27 words
A method of regulating the function of a gene in a prokaryotic or eukaryotic cell which comprises introducing into said cell the DNA construct of claim 5.
Claim 12
Dependent Claim
55 words
A method of regulating the function of a gene in a prokaryotic or eukaryotic cell which comprises: introducing into said cell the DNA construct of claim 5 whereby a transformed cell is obtained; and growing said transformed cell whereby the RNA produced by transcription of the inverted gene segment regulates the functioning of said gene.
Claim 13
Dependent Claim
96 words
A non-native polynucleotide construct which, when present in a cell containing a gene, produces an RNA which regulates the function of said gene, said polynucleotide construct containing the following operably linked polynucleotide segments: a. a transcriptional promoter segment; b. a transcription termination segment; and c. a polynucleotide segment comprising a segment of said gene, said gene segment located between said promoter segment and said termination segment and being inverted with respect to said promoter segment and said termination segment, whereby the RNA produced by transcription of the inverted gene segment regulates the function of said gene.
Claim 14
Dependent Claim
96 words
A non-native polynucleotide construct which, when present in a cell containing a gene, produces an RNA which regulates the function of said gene, said polynucleotide construct containing the following operably linked polynucleotide segments: a. a transcriptional promoter segment; b. a transcription termination segment; and c. a polynucleotide segment comprising a segment of said gene, said gene segment located between said promoter segment and said termination segment and being inverted with respect to said promoter segment and said termination segment, whereby the RNA produced by transcription of the inverted gene segment regulates the function of said gene.
Claim 15
Dependent Claim
11 words
The polynucleotide construct of claim 9 wherein said cell is prokaryotic.
Claim 16
Dependent Claim
11 words
The polynucleotide construct of claim 9 wherein said cell is eukaryotic.
Claim 17
Dependent Claim
15 words
A polynucleotide construct of claim 9 wherein said transcriptional promoter segment comprises an inducible promoter.
Claim 18
Dependent Claim
12 words
A polynucleotide construct of claim 9 wherein said gene is an oncogene.
Claim 19
Dependent Claim
13 words
A polynucleotide construct of claim 9 wherein said gene is a viral gene.
Claim 20
Dependent Claim
12 words
A polynucleotide construct of claim 9 wherein said gene encodes a protein.
Claim 21
Dependent Claim
15 words
A polynucleotide construct of claim 15 wherein said transcriptional promoter segment comprises an inducible promoter.
Claim 22
Dependent Claim
18 words
A polynucleotide construct of claim 15 wherein said gene segment includes the 5' non-coding region of said gene.
Claim 23
Dependent Claim
18 words
A polynucleotide construct of claim 15 wherein said gene segment includes the ribosome binding portion of said gene.
Claim 24
Dependent Claim
18 words
A polynucleotide construct of claim 15 wherein said gene segment includes the translation initiation portion of said gene.
Claim 25
Dependent Claim
15 words
A vector having incorporated therein a polynucleotide construct according to any one of claims 9-19.
Claim 26
Dependent Claim
12 words
A vector according to claim 20 wherein said vector is a plasmid.
Claim 27
Dependent Claim
13 words
A vector according to claim 20 wherein said vector is a viral vector.
Claim 28
Dependent Claim
14 words
A pharmaceutical composition which comprises the polynucleotide construct of any one of claims 9-19.
Claim 29
Dependent Claim
10 words
A pharmaceutical composition which comprises the vector of claim 20.
Claim 30
Dependent Claim
10 words
A pharmaceutical composition which comprises the vector of claim 21.
Claim 31
Dependent Claim
10 words
A pharmaceutical composition which comprises the vector of claim 22.
Claim 32
Independent Claim
29 words
A method of regulating the function of a gene in a cell which comprises introducing into said cell the polynucleotide construct of any one of claims 9 or 12-19.
Claim 33
Dependent Claim
23 words
A method of regulating the function of a gene in a cell which comprises introducing into said cell the vector of claim 20.
Claim 34
Dependent Claim
10 words
The method of claim 27 wherein said cell is prokaryotic.
Claim 35
Dependent Claim
10 words
The method of claim 27 wherein said cell is eukaryotic.
Claim 36
Dependent Claim
10 words
The method of claim 28 wherein said cell is prokaryotic.
Claim 37
Dependent Claim
10 words
The method of claim 28 wherein said cell is eukaryotic.
Claim 38
Independent Claim
55 words
A method of regulating the function of a gene in a cell which comprises: introducing into said cell the polynucleotide construct of any one of claims 9-19 whereby a transformed cell is obtained; and growing said transformed cell whereby the RNA produced by transcription of the inverted gene segment regulates the functioning of said gene.
Claim 39
Dependent Claim
89 words
A non-native polynucleotide construct which, when present in a cell containing a gene, produces an RNA which regulates the function of said gene, said polynucleotide construct containing the following operably linked polynucleotide segments: a. a transcriptional promoter segment; b. a transcription termination segment; and therebetween c. a polynucleotide segment; whereby transcription of the polynucleotide segment produces a ribonucleotide sequence which does not naturally occur in the cell, is complementary to a ribonucleotide sequence transcribed from said gene, and said non-naturally occurring ribonucleotide sequence regulates the function of said gene.
Claim 40
Dependent Claim
89 words
A non-native polynucleotide construct which, when present in a cell containing a gene, produces an RNA which regulates the function of said gene, said polynucleotide construct containing the following operably linked polynucleotide segments: a. a transcriptional promoter segment; b. a transcription termination segment; and therebetween c. a polynucleotide segment; whereby transcription of the polynucleotide segment produces a ribonucleotide sequence which does not naturally occur in the cell, is complementary to a ribonucleotide sequence transcribed from said gene, and said non-naturally occurring ribonucleotide sequence regulates the function of said gene.
Claim 41
Dependent Claim
11 words
The polynucleotide construct of claim 34 wherein said cell is prokaryotic.
Claim 42
Dependent Claim
11 words
The polynucleotide construct of claim 34 wherein said cell is eukaryotic.
Claim 43
Dependent Claim
15 words
A polynucleotide construct of claim 34 wherein said transcriptional promoter segment comprises an inducible promoter.
Claim 44
Dependent Claim
12 words
A polynucleotide construct of claim 34 wherein said gene is an oncogene.
Claim 45
Dependent Claim
13 words
A polynucleotide construct of claim 34 wherein said gene is a viral gene.
Claim 46
Dependent Claim
12 words
A polynucleotide construct of claim 34 wherein said gene encodes a protein.
Claim 47
Dependent Claim
15 words
A polynucleotide construct of claim 40 wherein said transcriptional promoter segment comprises an inducible promoter.
Claim 48
Dependent Claim
29 words
A polynucleotide construct of claim 40 wherein said polynucleotide segment encodes a ribonucleotide sequence complementary to a 5' end non-coding portion of said ribonucleotide sequence transcribed from said gene.
Claim 49
Dependent Claim
28 words
A polynucleotide construct of claim 40 wherein said polynucleotide segment encodes a ribonucleotide sequence complementary to a ribosome binding portion of said ribonucleotide sequence transcribed from said gene.
Claim 50
Dependent Claim
28 words
A polynucleotide construct of claim 40 wherein said polynucleotide segment encodes a ribonucleotide sequence complementary to the translation initiation portion of said ribonucleotide sequence transcribed from said gene.
Claim 51
Dependent Claim
15 words
A vector having incorporated therein a polynucleotide construct according to any one of claims 34-44.
Claim 52
Dependent Claim
12 words
A vector according to claim 45 wherein said vector is a plasmid.
Claim 53
Dependent Claim
13 words
A vector according to claim 45 wherein said vector is a viral vector.
Claim 54
Dependent Claim
14 words
A pharmaceutical composition which comprises the polynucleotide construct of any one of claims 34-44.
Claim 55
Dependent Claim
10 words
A pharmaceutical composition which comprises the vector of claim 45.
Claim 56
Dependent Claim
10 words
A pharmaceutical composition which comprises the vector of claim 46.
Claim 57
Dependent Claim
10 words
A pharmaceutical composition which comprises the vector of claim 47.
Claim 58
Dependent Claim
85 words
A cell containing a non-native polynucleotide construct, which construct produces an RNA which regulates the function of a gene, said polynucleotide construct containing the following operably linked polynucleotide segments: a. a transcriptional promoter segment; b. a transcription termination segment; and therebetween c. a polynucleotide segment; whereby transcription of the polynucleotide segment produces a ribonucleotide sequence which does not naturally occur in the cell, is complementary to a ribonucleotide sequence transcribed from said gene, and said non-naturally occurring ribonucleotide sequence regulates the function of said gene.
Claim 59
Dependent Claim
85 words
A cell containing a non-native polynucleotide construct, which construct produces an RNA which regulates the function of a gene, said polynucleotide construct containing the following operably linked polynucleotide segments: a. a transcriptional promoter segment; b. a transcription termination segment; and therebetween c. a polynucleotide segment; whereby transcription of the polynucleotide segment produces a ribonucleotide sequence which does not naturally occur in the cell, is complementary to a ribonucleotide sequence transcribed from said gene, and said non-naturally occurring ribonucleotide sequence regulates the function of said gene.
Claim 60
Dependent Claim
10 words
The cell of claim 52 wherein said cell is prokaryotic.
Claim 61
Dependent Claim
10 words
The cell of claim 52 wherein said cell is eukaryotic.
Claim 62
Dependent Claim
14 words
The cell of claim 52 wherein said transcriptional promoter segment comprises an inducible promoter.
Claim 63
Dependent Claim
11 words
The cell of claim 52 wherein said gene is an oncogene.
Claim 64
Dependent Claim
12 words
The cell of claim 52 wherein said gene is a viral gene.
Claim 65
Dependent Claim
11 words
The cell of claim 52 wherein said gene encodes a protein.
Claim 66
Dependent Claim
14 words
The cell of claim 58 wherein said transcriptional promoter segment comprises an inducible promoter.
Claim 67
Dependent Claim
27 words
The cell of claim wherein said polynucleotide segment encodes a ribonucleotide sequence complementary to a 5' end non-coding portion of said ribonucleotide sequence transcribed from said gene.
Claim 68
Dependent Claim
27 words
The cell of claim 58 wherein said polynucleotide segment encodes a ribonucleotide sequence complementary to a ribosome binding portion of said ribonucleotide sequence transcribed from said gene.
Claim 69
Dependent Claim
27 words
The cell of claim 58 wherein said polynucleotide segment encodes a ribonucleotide sequence complementary to the translation initiation portion of said ribonucleotide sequence transcribed from said gene.
Claim 70
Dependent Claim
18 words
The cell according to any one of claims 52-62 wherein said polynucleotide construct is incorporated into a vector.
Claim 71
Dependent Claim
12 words
The cell according to claim 63 wherein said vector is a plasmid.
Claim 72
Dependent Claim
13 words
The cell according to claim 63 wherein said vector is a viral vector.
Claim 73
Independent Claim
29 words
A method of regulating the function of a gene in a cell which comprises introducing into said cell the polynucleotide construct of any one of claims 34 or 37-44.
Claim 74
Dependent Claim
23 words
A method of regulating the function of a gene in a cell which comprises introducing into said cell the vector of claim 45.
Claim 75
Dependent Claim
10 words
The method of claim 66 wherein said cell is prokaryotic.
Claim 76
Dependent Claim
10 words
The method of claim 66 wherein said cell is eukaryotic.
Claim 77
Dependent Claim
10 words
The method of claim 67 wherein said cell is prokaryotic.
Claim 78
Dependent Claim
10 words
The method of claim 67 wherein said cell is eukaryotic.
Claim 79
Independent Claim
54 words
A method of regulating the function of a gene in a cell which comprises: introducing into said cell the polynucleotide construct of any one of claims 34-44 whereby a transformed cell is obtained; and growing said transformed cell whereby the RNA produced by transcription of said polynucleotide segment regulates the functioning of said gene.
Claim 80
Dependent Claim
92 words
A cell containing a non-native polynucleotide construct, which construct produces an RNA which regulates the function of a gene, said polynucleotide construct containing the following operably linked polynucleotide segments: a. a transcriptional promoter segment; b. a transcription termination segment; and c. a polynucleotide segment comprising a segment of said gene, said gene segment located between said promoter segment and said termination segment and being inverted with respect to said promoter segment and said termination segment, whereby the RNA produced by transcription of the inverted gene segment regulates the function of said gene.
Claim 81
Dependent Claim
92 words
A cell containing a non-native polynucleotide construct, which construct produces an RNA which regulates the function of a gene, said polynucleotide construct containing the following operably linked polynucleotide segments: a. a transcriptional promoter segment; b. a transcription termination segment; and c. a polynucleotide segment comprising a segment of said gene, said gene segment located between said promoter segment and said termination segment and being inverted with respect to said promoter segment and said termination segment, whereby the RNA produced by transcription of the inverted gene segment regulates the function of said gene.
Claim 82
Dependent Claim
10 words
The cell of claim 73 wherein said cell is prokaryotic.
Claim 83
Dependent Claim
10 words
The cell of claim 73 wherein said cell is eukaryotic.
Claim 84
Dependent Claim
14 words
The cell of claim 73 wherein said transcriptional promoter segment comprises an inducible promoter.
Claim 85
Dependent Claim
11 words
The cell of claim 73 wherein said gene is an oncogene.
Claim 86
Dependent Claim
12 words
The cell of claim 73 wherein said gene is a viral gene.
Claim 87
Dependent Claim
11 words
The cell of claim 73 wherein said gene encodes a protein.
Claim 88
Dependent Claim
14 words
The cell of claim 79 wherein said transcriptional promoter segment comprises an inducible promoter.
Claim 89
Dependent Claim
17 words
The cell of claim 79 wherein said gene segment includes the 5' non-coding region of said gene.
Claim 90
Dependent Claim
17 words
The cell of claim 79 wherein said gene segment includes the ribosome binding portion of said gene.
Claim 91
Dependent Claim
17 words
The cell of claim 79 wherein said gene segment includes the translation initiation portion of said gene.
Claim 92
Dependent Claim
17 words
The cell of any one of claims 79-83 wherein the polynucleotide construct is incorporated into a vector.
Claim 93
Dependent Claim
11 words
The cell of claim 84 wherein said vector is a plasmid.
Claim 94
Dependent Claim
12 words
The cell of claim 84 wherein said vector is a viral vector.
References Cited (Backward Citations)
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