US4912040A
Expired / Lapsed
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Eucaryotic expression system
Assignee: Genetics Institute LLC
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Filing: 1987-02-03
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Granted: 1990-03-27
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Expires: 2007-02-03
Abstract
Abstract This invention provides vectors, improved host cells and improved methods for producing a heterologous protein by culturing an improved eucaryotic host cell of this invention transformed or transfected with a vector capable of directing the expression of the heterologous protein. The preferred improved host cell of this invention is a mammalian host cell containing and capable of expressing an anti-sense GRP78 DNA sequence.
Inventors
- 👤 Randal J. Kaufman
- 👤 Andrew Dorner
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 01:44
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C07; C07K; C07K14/00; C07K14/435; C07K14/705; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456; C12N9/6459; C12Y; C12Y304/00; C12Y304/21; C12Y304/21069
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
55 words
An anti-sense expression vector capable of directing the transcription in a host cell of mRNA complementary to mRNA encoding the host cell's GRP78, said vector comprising a DNA sequence encoding host cell GRP78 peptide sequence operatively linked to an expression control sequence in reverse orientation such that transcription of the DNA produces the anti-sense mRNA.
Claim 2
Dependent Claim
55 words
An anti-sense expression vector capable of directing the transcription in a host cell of mRNA complementary to mRNA encoding the host cell's GRP78, said vector comprising a DNA sequence encoding host cell GRP78 peptide sequence operatively linked to an expression control sequence in reverse orientation such that transcription of the DNA produces the anti-sense mRNA.
Claim 3
Dependent Claim
24 words
A eucaryotic host cell for expressing a protein heterologous to the cell which comprises a host cell transformed with a vector of claim 1.
Claim 4
Dependent Claim
12 words
A yeast, fungal, insect, plant or mammalian host cell of claim 2.
Claim 5
Dependent Claim
25 words
A host cell of claim 2 which is additionally transformed with a vector capable of directing the expression of a protein heterologous to the cell.
Claim 6
Dependent Claim
18 words
A host cell of claim 4, wherein the protein heterologous to the cell is a factor VIII protein.
Claim 7
Dependent Claim
45 words
A method for producing a protein heterologous to a host cell by culturing a host cell transformed with a vector capable of directing the expression of the heterologous protein, the improvement comprising culturing a transformed host cell of claim 4 as the transformed host cell.
Claim 8
Dependent Claim
32 words
A method for producing a factor VIII protein which comprises culturing the improved host cell of claim 5 and recovering from the culture medium the Factor VIII protein so produced and secreted.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-03-26 | 1993-09-25 | 1994-03-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-03-26 | 1997-09-25 | 1998-03-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-03-26 | 2001-09-25 | 2002-03-27 | $7700.00 | Window Closed / Lapsed |