Patent US6277576B1 successfully refreshed from Google Patents.
US6277576B1
Expired / Lapsed
US
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Nucleic acid mediated electron transfer
Assignee: California Institute of Technology
•
Filing: 1999-05-07
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Granted: 2001-08-21
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Expires: 2019-05-07
Abstract
Abstract The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.
Inventors
- 👤 Thomas J. Meade
- 👤 Jon Faiz Kayyem
- 👤 Scott E. Fraser
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 22:23
CPC Classifications
C; C07; C07H; C07H21/00; C07H23/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/6818; C12Q1/6825; Y; Y02; Y02A; Y02A50/00; Y02A50/30
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
76 words
A method for detecting the presence of a target sequence in a nucleic acid sample comprising: a) applying an AC input signal to a hybridization complex, wherein said hybridization complex comprises: i) a first single stranded nucleic acid comprising an electrode; and ii) a target sequence containing at least one moiety capable of donating or accepting an electron; and b) detecting electron transfer between said moiety capable of donating or accepting an electron and said electrode.
Claim 2
Independent Claim
76 words
A method for detecting the presence of a target sequence in a nucleic acid sample comprising: a) applying an AC input signal to a hybridization complex, wherein said hybridization complex comprises: i) a first single stranded nucleic acid comprising an electrode; and ii) a target sequence containing at least one moiety capable of donating or accepting an electron; and b) detecting electron transfer between said moiety capable of donating or accepting an electron and said electrode.
Claim 3
Independent Claim
81 words
A method for detecting the presence of a target sequence in a nucleic acid sample comprising: a) applying an AC input signal to a hybridization complex, wherein said hybridization complex comprises: i) an electron transfer mediator; ii) a first single stranded nucleic acid comprising an electrode; and iii) a target sequence containing at least one moiety capable of donating or accepting an electron; and b) detecting electron transfer between said moiety capable of donating or accepting an electron and said electrode.
Claim 4
Independent Claim
81 words
A method for detecting the presence of a target sequence in a nucleic acid sample comprising: a) applying an AC input signal to a hybridization complex, wherein said hybridization complex comprises: i) an electron transfer mediator; ii) a first single stranded nucleic acid comprising an electrode; and iii) a target sequence containing at least one moiety capable of donating or accepting an electron; and b) detecting electron transfer between said moiety capable of donating or accepting an electron and said electrode.
Claim 5
Dependent Claim
20 words
A method according to claim 1 or 2 wherein said AC input signal is applied at a plurality of frequencies.
Claim 6
Dependent Claim
28 words
A method according to claim 1 or 2 wherein said detecting is done by receiving an output signal characteristic of electron transfer between said moiety and said electrode.
Claim 7
Dependent Claim
13 words
A method according to claim 4 wherein said output signal is a current.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2004-08-20 | 2005-02-19 | 2005-08-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2008-08-20 | 2009-02-19 | 2009-08-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2012-08-20 | 2013-02-19 | 2013-08-21 | $7700.00 | Window Closed / Lapsed |