US6040193A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Combinatorial strategies for polymer synthesis
Assignee: Affymetrix Inc
•
Filing: 1998-08-04
•
Granted: 2000-03-21
•
Expires: 2018-08-04
Abstract
Abstract An array is created by moving a dispenser toward a solid support until a tip of the dispenser touches the support, withdrawing the tip from the surface and releasing a drop of 5 nanoliters or less in an area smaller than 1 mm2 to create an array of at least 100 spots.
Inventors
- 👤 James L. Winkler
- 👤 Stephen P. A. Fodor
- 👤 Christopher J. Buchko
- 👤 Debra A. Ross
- 👤 Lois Aldwin
- 👤 Douglas N. Modlin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 02:48
CPC Classifications
B; B01; B01J; B01J19/00; B01J19/0046; B82; B82Y; B82Y30/00; C; C07; C07H; C07H21/00; C07K; C07K1/00; C07K1/04; C07K1/045; C07K1/047; C07K5/00; C07K5/04; C07K5/10; C07K5/1002; C07K5/1005; C07K5/1008; C07K5/1016; G; G01; G01N; G01N35/00; G01N35/10; G01N35/1065; G01N35/1072; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00351; B01J2219/00364; B01J2219/00367; B01J2219/00369; B01J2219/00378; B01J2219/00389; B01J2219/00414; B01J2219/00416; B01J2219/00427; B01J2219/0043; B01J2219/00432; B01J2219/00457; B01J2219/00475; B01J2219/00497; B01J2219/005; B01J2219/00527; B01J2219/00529; B01J2219/00531; B01J2219/00536; B01J2219/00583; B01J2219/00585; B01J2219/0059; B01J2219/00596; B01J2219/00603; B01J2219/00605; B01J2219/00608; B01J2219/0061; B01J2219/00612; B01J2219/00614; B01J2219/00617; B01J2219/00621; B01J2219/00623; B01J2219/00626; B01J2219/00632; B01J2219/00637; B01J2219/00659; B01J2219/00675; B01J2219/0068; B01J2219/00686; B01J2219/00689; B01J2219/00702; B01J2219/00709; B01J2219/00711; B01J2219/00718; B01J2219/0072; B01J2219/00722; B01J2219/00725; C07B; C07B2200/00; C07B2200/11; C40; C40B; C40B40/00; C40B40/04; C40B40/06; C40B40/10; C40B50/00; C40B50/14; C40B60/00; C40B60/14; Y; Y02; Y02P; Y02P20/00; Y02P20/50; Y02P20/55; Y10; Y10S; Y10S435/00; Y10S435/97; Y10S435/973; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333; Y10T436/25; Y10T436/2575
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
115 words
A method of forming an array of polymers on a solid support, comprising: (a) moving a dispenser containing a solution of a polymer toward a surface of a solid support until a tip of the dispenser touches the surface, moving the dispenser away from the surface and releasing a droplet of 5 nl or less from the dispenser, the droplet contacting the surface at a localized area smaller than 1 mm 2 , whereby the polymer attaches to the surface of the support at the localized area; (b) repeating (a) to attach a different polymer at a different localized area until an array of at least 100 different polymers at different localized areas is formed.
Claim 2
Independent Claim
115 words
A method of forming an array of polymers on a solid support, comprising: (a) moving a dispenser containing a solution of a polymer toward a surface of a solid support until a tip of the dispenser touches the surface, moving the dispenser away from the surface and releasing a droplet of 5 nl or less from the dispenser, the droplet contacting the surface at a localized area smaller than 1 mm 2 , whereby the polymer attaches to the surface of the support at the localized area; (b) repeating (a) to attach a different polymer at a different localized area until an array of at least 100 different polymers at different localized areas is formed.
Claim 3
Dependent Claim
11 words
The method of claim 1 wherein said dispenser comprises a capillary.
Claim 4
Dependent Claim
16 words
The method of claim 1 wherein said localized areas are spaced between 5 and 100 microns.
Claim 5
Dependent Claim
22 words
The method of claim 1 wherein said surface comprises a hydrophobic material surrounding hydrophilic localized areas to which the polymers are attached.
Claim 6
Dependent Claim
11 words
The method of claim 1 wherein said dispenser comprises a micropipette.
Claim 7
Dependent Claim
11 words
The method of claim 1, wherein the polymers comprise nucleic acids.
Claim 8
Dependent Claim
14 words
The method of claim 6 wherein said nucleic acids are obtained from cell extracts.
Claim 9
Dependent Claim
35 words
The method of claim 6, wherein (a) and (b) are repeated to produce an array of at least 1000 different nucleic acids occupying localized areas within 1 cm 2 of the surface of the support.
Claim 10
Dependent Claim
13 words
The method of claim 1, wherein the support is glass, quartz or silicon.
Claim 11
Dependent Claim
15 words
The method of claim 1, wherein the solution of the polymer is an aqueous solution.
Claim 12
Dependent Claim
31 words
The method of claim 1, wherein the support bears at least two reference points for positioning the dispenser over at least one of said localized areas for release of said droplet.
Claim 13
Dependent Claim
51 words
The method of claim 11, wherein the reference points comprise global reference points for positioning the dispenser over a local region of the surface of the support, and local reference points within the local region for positioning the dispenser over at least one of said localized areas within the local region.
Claim 14
Dependent Claim
17 words
The method of claim 12, wherein the dispenser further comprises a camera for identifying the reference points.
Claim 15
Dependent Claim
19 words
The method of claim 1, wherein the polymers are each at least 20% pure within their respective localized areas.
References Cited (Backward Citations)
US3615240A
Cited
US3848998A
Cited
US4166457A
Cited
US4392362A
Cited
US4282287A
Cited
US4327073A
Cited
US4704256A
Cited
US4378333A
Cited
US4711955A
Cited
EP0063810A1
Cited
US5486452A
Cited
US4483920A
Cited
US4689405A
Cited
US4994373A
Cited
US4591570A
Cited
US4584277A
Cited
US4562157A
Cited
US4987065A
Cited
US4542102A
Cited
US4677054A
Cited
US4598049A
Cited
US4746490A
Cited
US4849513A
Cited
US4613566A
Cited
US4737344A
Cited
GB2156074B
Cited
US4668476A
Cited
US4704353A
Cited
US4728502A
Cited
US4861420A
Cited
EP0171150B1
Cited
US4755458A
Cited
EP0173339B1
Cited
US4586546A
Cited
US4820630A
Cited
EP0185547B1
Cited
US5026840A
Cited
US4681870A
Cited
US4675301A
Cited
US4833092A
Cited
US5043265A
Cited
EP0225807B1
Cited
EP0232967B1
Cited
US5447841A
Cited
US4855225A
Cited
US4868103A
Cited
US5348855A
Cited
EP0235726B1
Cited
US4925785A
Cited
US4728591A
Cited
EP0237362B2
Cited
US4818492A
Cited
US5310893A
Cited
US4937188A
Cited
US4981783A
Cited
US4966646A
Cited
US4943032A
Cited
US4877745A
Cited
US5028525A
Cited
US5175209A
Cited
US4762881A
Cited
US4861866A
Cited
US5077085A
Cited
US5079600A
Cited
US5037882A
Cited
EP0281927B1
Cited
US5026773A
Cited
US5667972A
Cited
US5525464A
Cited
US5492806A
Cited
US5695940A
Cited
US5202231A
Cited
US4997521A
Cited
US4874500A
Cited
US5011770A
Cited
US4923901A
Cited
US5082830A
Cited
US4988617A
Cited
EP0337498A2
Cited
US5002867A
Cited
US5700637A
Cited
US5436327A
Cited
US5200051A
Cited
US5489507A
Cited
US5077210A
Cited
US5053454A
Cited
EP0392546A3
Cited
US5405783A
Cited
US5510270A
Cited
US5445934A
Cited
US5143854A
Cited
US5279558A
Cited
GB2233654A
Cited
US5232829A
Cited
US5215882A
Cited
US5094594A
Cited
US5100626A
Cited
US5338688A
Cited
US5384262A
Cited
US5143849A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2003-03-21 | 2003-09-20 | 2004-03-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2007-03-21 | 2007-09-20 | 2008-03-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2011-03-21 | 2011-09-20 | 2012-03-21 | $7700.00 | Window Closed / Lapsed |