EP0171150B1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Method and apparatus for assaying with optional reagent quality control
Assignee: Orgenics Ltd
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Filing: 1985-06-12
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Granted: 1992-03-25
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Expires: 2005-06-12
Abstract
Abstract A solid phase immuno-assay system and method for detect ing and measuring at least one analyte in a single sample. The system includes a solid support having a plurality of receptors bound thereto. The support may be selectively treated whereby upon exposure to a sample, and after subsequent processing, the support exhibits a standard reference curve which may be compared with another portion of the card for reference purposes. Alternatively, or additionally, the support may be selectively treated with a control substance to monitor the activity of at least one reagent in the procedure. An apparatus which may be used for performing the multi-step assay procedure which includes a container having a plurality of compartments therein, each containing a reagent for the assay. A method of detecting the presence of horseradish peroxidase by contacting a substance suspected of containing horseradish peroxidase with potassium iodide, starch and hydrogen peroxide.
Inventors
- 👤 Max Herzberg
- 👤 Falk Fish
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-26 05:06
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/508; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/528; G01N33/53; G01N33/543; G01N33/54366
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
220 words
A method for detecting at least one analyte in a single liquid solution suspected of containing more than one analyte, said method comprising the steps of: (a) providing a solid support which is water insoluble and flexible and made from material selected from the group of polyvinyl, polystyrene, cellulose, nylon or glass, shaped in a form of flat, rectangular or round sheet, a rod, a stick or a cylinder; (b) attaching and selectively positioned on said support at least one receptor for said at least one analyte, said receptor being selected from the group consisting of proteins, immunoglobins, nucleic acids or polysaccharides, carbohydrates, lipids; (c) placing at least one control spot on a pre-designated area of said solid support for monitoring at least one reaction; (d) swabbing said liquid solution on said support (c), or dipping said support (c) into said liquid solution, wherein said liquid solution contains at least one analyte selected from the group consisting of proteins, nucleic acids, carbohydrates, immunoglobins, polysaccharides, lipids to form receptor-analyte conjugate reaction; (e) contacting said receptor-analyte conjugate reaction (d) with a signal generating reagent to determine the presence and/or extent of said at least one analyte, wherein said signal generating reagents are labelled probes selected from the group consisting of antibodies, antigens, enzymes, carbohydrates, polysaccharides, antigens and nucleic acids, proteins and lipids.
Claim 2
Independent Claim
220 words
A method for detecting at least one analyte in a single liquid solution suspected of containing more than one analyte, said method comprising the steps of: (a) providing a solid support which is water insoluble and flexible and made from material selected from the group of polyvinyl, polystyrene, cellulose, nylon or glass, shaped in a form of flat, rectangular or round sheet, a rod, a stick or a cylinder; (b) attaching and selectively positioned on said support at least one receptor for said at least one analyte, said receptor being selected from the group consisting of proteins, immunoglobins, nucleic acids or polysaccharides, carbohydrates, lipids; (c) placing at least one control spot on a pre-designated area of said solid support for monitoring at least one reaction; (d) swabbing said liquid solution on said support (c), or dipping said support (c) into said liquid solution, wherein said liquid solution contains at least one analyte selected from the group consisting of proteins, nucleic acids, carbohydrates, immunoglobins, polysaccharides, lipids to form receptor-analyte conjugate reaction; (e) contacting said receptor-analyte conjugate reaction (d) with a signal generating reagent to determine the presence and/or extent of said at least one analyte, wherein said signal generating reagents are labelled probes selected from the group consisting of antibodies, antigens, enzymes, carbohydrates, polysaccharides, antigens and nucleic acids, proteins and lipids.
Claim 3
Dependent Claim
16 words
The method as claimed in claim 1 wherein said signal generating reagents produce a chromogenic reaction.
Claim 4
Independent Claim
38 words
The method as claimed in claims 1 or 2 wherein said support is a flat sheet, and said sheet comprises a tear line, whereby a portion of said sheet may be separated from the remainder of said sheet.
Claim 5
Independent Claim
36 words
The method as claimed in claims 1, 2 or 3 wherein the said support further comprises standard spots thereon for establishing standard values, and wherein said method comprises comparing said standard values with the results observed.
Claim 6
Independent Claim
35 words
The method as claimed in claims 1, 2, 3, or 4 wherein said labelled probe is a receptor, whereby a sandwich is formed by said labelled probe, said analyte, and said receptor on said support.
Claim 7
Independent Claim
20 words
The method as claimed in any one of the preceding claims wherein said labelled probe is labelled with picryl sulfonate.
Claim 8
Independent Claim
64 words
The method as claimed claims 4, 5 or 6 wherein said support comprises a tear line to allow for separation of a portion of said support, and wherein said standard spots are all located to one side of said tear line whereby the portion of said support having said standard spots thereon may be separated from the remainder of said support and compared thereto.
Claim 9
Independent Claim
18 words
The method as claimed in any one of the preceding claims wherein said probe is monitored for activity.
Claim 10
Independent Claim
32 words
The method as claimed in any one of the preceding claims wherein said labelled probe is labelled with a member selected from the group consisting of an enzyme or radio active substance.
Claim 11
Dependent Claim
52 words
The method as claimed in claim 9 wherein said label is an enzyme, and said method further comprises contacting said support with a dye and substrate after exposure to said liquid solution and said labelled probe to provide a colour reaction permitting observation of the locations at which said analyte is bound.
Claim 12
Dependent Claim
26 words
The method as claimed in claim 10 comprising adding a gelling agent to said support and developing said colour reaction in said gel on said support.
Claim 13
Dependent Claim
31 words
The method as claimed in claim 10 comprising contacting said support with the developing sheet having a gel thereon, and developing said colour reaction on said developing sheet in said gel.
Claim 14
Dependent Claim
22 words
The method as claimed in claim 10 wherein said enzyme is selected from the group consisting of peroxidase, glucose oxidase and ,8-galactosidase.
Claim 15
Independent Claim
29 words
The method as claimed in any one of the preceding claims comprising amplifying the number of binding sites on said support prior to contacting said support with said receptors.
Claim 16
Independent Claim
30 words
The method as claimed in any one of the preceding claims wherein said support is a sheet which comprises a tear line to facilitate separation of portion of said sheet.
Claim 17
Independent Claim
31 words
The method as claimed in any one of the preceding claims comprising washing said support after contacting said liquid solution with said support to remove excess unbound material from said support.
Claim 18
Independent Claim
67 words
The method as claimed in any one of the preceding claims comprising providing a container having a plurality of compartments, the number of compartments corresponding to the number of steps performed, each compartment having a specific reagent or group of reagents therein, and performing said method by dipping said support within each of said compartments to contact said support with the reagent within each of said compartments.
Claim 19
Independent Claim
33 words
An apparatus for performing said method according to any one of the preceding claims comprising a container having a plurality of compartments therein, each of said compartments containing a reagent for said assay.
Claim 20
Dependent Claim
29 words
The apparatus as claimed in claim 18 wherein each of said compartments is elongated and has an elongate opening at its top through which a card may be inserted.
Claim 21
Dependent Claim
53 words
The apparatus as claimed in claim 19 wherein each of said elongated openings is covered with an elastic cover having an elongate slit therein to form two lips over each of said compartments through which a sheet may be inserted and removed with said lips serving to remove excess material from said sheet.
Claim 22
Dependent Claim
17 words
The apparatus as claimed in claim 20 wherein each of said compartments contains reagent in dehydrated form.
Claim 23
Dependent Claim
30 words
The apparatus as claimed in claim 21 wherein one wall of each said compartments has an orifice therein through which liquid may be injected to hydrate each of said reagents.
Claim 24
Dependent Claim
18 words
The apparatus as claimed in claim 22 wherein each of said orifices is covered with a sealing strip.
Claim 25
Dependent Claim
76 words
A support for performing said method according to any one of the preceding claims whereby upon exposure to a liquid solution, and after subsequent processing, said support exhibits a standard reference curve which may be compared with another portion of said card for reference purposes and is formed by a series of discrete treated standard spots on said support, each of said standard spots exhibiting a different colour after exposure to said sample and final processing.
Claim 26
Dependent Claim
18 words
The support as claimed in claim 24 wherein said support is flexible card comprising a tear line thereon.
Claim 27
Dependent Claim
54 words
The support as claimed in claim 25 wherein all said standard spots are to one side of said tear line on a portion of said card whereby upon separation of said portion of said card along said tear line, said standard curve can be compared with the remainder of said card for reference purposes.
Claim 28
Dependent Claim
30 words
The support as claimed in claim 24 wherein said card has a plurality of receptors thereon adapted to bind more than a single analyte on separation location on said card.
Claim 29
Dependent Claim
30 words
The support as claimed in claim 24 wherein said support is made of a material selected from the group consisting of: polyvinyl, polystyrene, celulose, nylon, glass fibre, or mixtures thereof.
References Cited (Backward Citations)
No citation records indexed.
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