US5879885A
Expired / Lapsed
US
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Compositions for detecting and quantifying biological samples
Assignee: Gen Probe Inc
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Filing: 1995-06-07
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Granted: 1999-03-09
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Expires: 2015-06-07
Abstract
Abstract Methods and compositions for selectively detecting analytes in a homogeneous assay, a heterogeneous assay, or a mixture of the two by contacting a labeled probe:analyte complex with one or more amphiphiles. The invention is also useful for increasing the signal to noise ratio when used in conjunction with other assay systems. In preferred embodiments, the analyte and probe are nucleic acids or proteins.
Inventors
- 👤 Michael McClellan Becker
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 20:52
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/585; G01N33/586; C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; G01N33/53; G01N33/543; G01N33/54313; G01N33/5432; Y; Y10; Y10S; Y10S436/00; Y10S436/829
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
93 words
A composition for determining the presence or amount of a target analyte in a medium comprising: a) a labeled probe able to form a probe:analyte complex with said target analyte, and b) at least one amphiphile which is capable of altering the detectability of said probe which has not formed part of a probe:analyte complex, wherein the detectability of said probe which has formed part of said probe:analyte complex can be distinguished from the detectability of said probe which has not formed part of a probe:analyte complex in the presence of said amphiphile.
Claim 2
Dependent Claim
93 words
A composition for determining the presence or amount of a target analyte in a medium comprising: a) a labeled probe able to form a probe:analyte complex with said target analyte, and b) at least one amphiphile which is capable of altering the detectability of said probe which has not formed part of a probe:analyte complex, wherein the detectability of said probe which has formed part of said probe:analyte complex can be distinguished from the detectability of said probe which has not formed part of a probe:analyte complex in the presence of said amphiphile.
Claim 3
Dependent Claim
24 words
The composition of claim 1 wherein said label is detected as a result of a triggering reaction between the label and a triggering agent.
Claim 4
Dependent Claim
54 words
The composition of claim 1 wherein said label forms a detectable chemiluminescent or electrochemiluminescent species upon undergoing a triggering reaction and the label joined to the unconjugated probe is protected from the triggering agent by the presence of said amphiphile or amphiphiles under conditions permitting the triggering of label joined to said probe:analyte conjugate.
Claim 5
Dependent Claim
23 words
The composition of claim 1 wherein at least one amphiphile has a positive charge under conditions allowing the formation of said probe:analyte complex.
Claim 6
Dependent Claim
11 words
The composition of claim 1 containing at least two different amphiphiles.
Claim 7
Dependent Claim
11 words
The composition of claim 4 containing at least two different amphiphiles.
Claim 8
Dependent Claim
16 words
The composition of claim 5 wherein at least two of said amphiphiles have different hydrophobic domains.
Claim 9
Dependent Claim
16 words
The composition of claim 6 wherein at least two of said amphiphiles have different hydrophobic domains.
Claim 10
Dependent Claim
15 words
The composition of claim 1 wherein the probe and the analyte are each nucleic acids.
Claim 11
Dependent Claim
26 words
The composition of claim 2 wherein said label forms a detected chemiluminescent species after undergoing said triggering reaction, said species comprising an optionally substituted, excited N-acridone.
Claim 12
Dependent Claim
29 words
The composition of claim 10 wherein said label comprises an N-acridinium phenyl ester optionally substituted at the acridinium ring and at carbons 2 through 6 of the phenyl ring.
Claim 13
Dependent Claim
15 words
The composition of claim 10 wherein the probe and the analyte are each nucleic acids.
Claim 14
Dependent Claim
13 words
The composition of claim 2 wherein said triggering agent is an oxidizing agent.
Claim 15
Dependent Claim
30 words
The composition of claim 1 wherein said a first amphiphile is selected from the group consisting of the TRITON® series, the Tween series, the Brij series and the NP series.
Claim 16
Dependent Claim
26 words
The composition of claim 1 wherein a first amphiphile is selected from the group consisting of TRITON® X-100, TRITON® X-305, Tween 20, Brij 35 and NP-40.
Claim 17
Dependent Claim
53 words
The composition of claim 1 wherein a first amphiphile is selected from the group consisting of cetyl trimethyl ammonium salts, cetylpyridinium salts, cetyl dimethylethyl ammonium salts, 3-(cyclohexylamino)-2-hydroxyl-1-propane sulfonate salts, benzthonium salts, benzyldimethyl salts, benzyldimethyl stearyl ammonium salts, benzyl trimethyl ammonium salts, benzylcetyldimethyl ammonium salts, benzyldimethyltetradecyl ammonium salts, benzalkonium salts, and dodecyl sulfate salts.
Claim 18
Dependent Claim
57 words
The composition of claim 5 wherein a first amphiphile is TRITON® X-100 and a second amphiphile is selected from the group consisting of cetyl trimethyl ammonium salts, cetylpyridinium salts, cetyl dimethylethyl ammonium salts, 3-(cyclohexylamino)-2-hydroxyl-1-propane sulfonate salts, benzthonium salts, benzyldimethyl salts, benzyldimethyl stearyl ammonium salts, benzyl trimethyl ammonium salts, benzylcetyldimethyl ammonium salts, benzyldimethyltetradecyl ammonium salts and benzalkonium salts.
Claim 19
Dependent Claim
30 words
The composition of claim 5 wherein a first amphiphile is TRITON® X-100 and a second amphiphile is selected from the group consisting of cetyl trimethyl ammonium salts and cetylpyridinium salts.
Claim 20
Dependent Claim
34 words
The composition of claim 11 wherein said label is selected from the group consisting of standard acridinium ester, o-diMe acridinium ester, 1-Me acridinium ester, 2,7-diMe acridinium ester, naphthyl acridinium ester, and o-dibromo acridinium ester.
Claim 21
Dependent Claim
34 words
The composition of claim 17 wherei said label is selected from the group consisting of standard acridinium ester, o-diMe acridinium ester, 1-Me acridinium ester, 2,7-diMe acridinium ester, naphthyl acridinium ester, and o-dibromo acridinium ester.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-03-08 | 2002-09-07 | 2003-03-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-03-08 | 2006-09-07 | 2007-03-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-03-08 | 2010-09-07 | 2011-03-09 | $7700.00 | Window Closed / Lapsed |