US4318707A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Macromolecular fluorescent quencher particle in specific receptor assays
Assignee: Siemens Healthcare Diagnostics GmbH Germany
•
Filing: 1980-06-03
•
Granted: 1982-03-09
•
Expires: 2000-06-03
Abstract
Abstract Homogeneous immunoassay employing a light absorbing particle to which a member of an immunological pair (mip)-ligand and antiligand is bound and a labeled mip, where the label is light emitting. The labeled mip becomes bound to the light absorbing particle in proportion to the amount of analyte in the assay medium. Thus, the light emitted from the medium is related to the amount of analyte in the medium.
Inventors
- 👤 David J. Litman
- 👤 Zvi Harel
- 👤 Edwin F. Ullman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 20:34
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/582; G01N33/581
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
203 words
An assay method for determining the presence in a sample of an analyte which is a mip, wherein said mip is a member of a specific binding pair consisting of ligand and its homologous antiligand; said method employing: (a) a continuous aqueous medium; (b) a particle conjugate comprising a mip bound to a light absorbing particle; (c) a signal label conjugate, comprising a signal label conjugated to a mip, wherein said signal label is a fluorescer of chemiluminescer; wherein the amount of signal label conjugate bound to said particle conjugate is related to the amount of analyte in said aqueous medium; said method comprising: combining in an aqueous medium, (a) said sample; (b) said particle conjugate substantially uniformly dispersed in said medium; (c) said signal label conjugate; (d) the homologous member of said specific binding pair, where the analyte, particle conjugate, and signal label conjugate are the same member of said specific binding pair; and (e) when the label is excited by chemical activation, additional reagents necessary for the production of chemiluminescence; and determining the amount of light emission from said assay medium containing both bound and unbound signal label conjugate as compared to an assay medium having a known amount of analyte.
Claim 2
Dependent Claim
203 words
An assay method for determining the presence in a sample of an analyte which is a mip, wherein said mip is a member of a specific binding pair consisting of ligand and its homologous antiligand; said method employing: (a) a continuous aqueous medium; (b) a particle conjugate comprising a mip bound to a light absorbing particle; (c) a signal label conjugate, comprising a signal label conjugated to a mip, wherein said signal label is a fluorescer of chemiluminescer; wherein the amount of signal label conjugate bound to said particle conjugate is related to the amount of analyte in said aqueous medium; said method comprising: combining in an aqueous medium, (a) said sample; (b) said particle conjugate substantially uniformly dispersed in said medium; (c) said signal label conjugate; (d) the homologous member of said specific binding pair, where the analyte, particle conjugate, and signal label conjugate are the same member of said specific binding pair; and (e) when the label is excited by chemical activation, additional reagents necessary for the production of chemiluminescence; and determining the amount of light emission from said assay medium containing both bound and unbound signal label conjugate as compared to an assay medium having a known amount of analyte.
Claim 3
Dependent Claim
49 words
The method according to claim 1, wherein said signal label is a fluorescer, said light absorbing particle is carbon and said aqueous assay medium is at a temperature in the range of about 10° to 50° C. and at a pH in the range of about 5 to 10.
Claim 4
Independent Claim
14 words
A method according to claims 1 or 2, wherein said analyte is a protein.
Claim 5
Independent Claim
17 words
A method according to claims 1 or 2, wherein said particle conjugate has antiligand as said mip.
Claim 6
Independent Claim
21 words
A method according to claims 1 or 2, wherein said fluorescer absorbs light at a wavelength greater than about 350 nm.
Claim 7
Dependent Claim
15 words
A method according to claim 3, wherein said particle conjugate has antiligand as said mip.
Claim 8
Dependent Claim
19 words
A method according to claim 3, wherein said fluorescer absorbs light at a wavelength greater than about 350 nm.
Claim 9
Dependent Claim
19 words
A method according to claim 6, wherein said fluorescer absorbs light at a wavelength greater than about 350 nm.
References Cited (Backward Citations)
US3791932A
Cited
US3901654A
Cited
US3853987A
Cited
DE2332697C3
Cited
US4059685A
Cited
US3998943A
Cited
US3935074A
Cited
US4052010A
Cited
US3996345A
Cited
US4199559A
Cited
US4174384A
Cited
US4104029A
Cited
US4201763A
Cited
US4067959A
Cited
US4158135A
Cited
US4134792A
Cited
US4160645A
Cited
US4208479A
Cited
US4220450A
Cited
US4233402A
Cited
US4193983A
Cited
US4238195A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-03-08 | 1985-09-07 | 1986-03-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-03-08 | 1989-09-07 | 1990-03-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-03-08 | 1993-09-07 | 1994-03-09 | $7700.00 | Window Closed / Lapsed |