US5093270A
Expired / Lapsed
US
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Method for enhancing signal release from liposomes using water-miscible alcohols
Assignee: Bayer Corp
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Filing: 1989-05-24
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Granted: 1992-03-03
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Expires: 2009-05-24
Abstract
Abstract A method for releasing encapsulated marker molecules from within liposomes comprises contracting the liposomes with an effective amount of a water-miscible alcohol. The chemiluminescence of acridinium esters is enhanced by oxidizing the acridinium ester in the presence of an effective amount of a water-miscible alcohol.
Inventors
- 👤 Steve C. S. Chang
- 👤 Say-Jong Law
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 06:19
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/5436; Y; Y10; Y10S; Y10S435/00; Y10S435/968; Y10S436/00; Y10S436/829
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
47 words
A method for enhancing the chemiluminescent signal of an acridinium ester in a chemiluminescent reaction which comprises oxidizing the acridinium ester under alkaline pH conditions in the presence of about 10 to 90% of a water-miscible alcohol wherein the water-miscible alcohol contains one to three carbon atoms.
Claim 2
Independent Claim
47 words
A method for enhancing the chemiluminescent signal of an acridinium ester in a chemiluminescent reaction which comprises oxidizing the acridinium ester under alkaline pH conditions in the presence of about 10 to 90% of a water-miscible alcohol wherein the water-miscible alcohol contains one to three carbon atoms.
Claim 3
Dependent Claim
15 words
A method as recited in claim 1 wherein the water-miscible alcohol contains one hydroxyl group.
Claim 4
Dependent Claim
14 words
A method as recited in claim 2 wherein the water-miscible alcohol is an isopropanol.
Claim 5
Dependent Claim
26 words
A method as recited in claim 1 wherein the effective amount of the water-miscible alcohol in the chemiluminescent reaction mixture is at least 10% by volume.
Claim 6
Dependent Claim
26 words
A method as recited in claim 4 wherein the effective amount of the water-miscible alcohol in the chemiluminescent reaction mixture is at least 50% by volume.
Claim 7
Independent Claim
89 words
A method for enhancing the chemiluminescent signal from an acridinium ester encapsulated within a liposome vesicle, in a chemiluminescent reaction which comprises: a) contacting the liposome vesicle with an aqueous solution comprising about 10 to 90% of a first water-miscible alcohol to release the acridinium ester from the liposome vesicle; and b) oxidizing the acridinium ester so released under alkaline pH conditions in the presence of about 10 to 90% of a second water-miscible alcohol wherein the first and second water-miscible alcohols each contain one to three carbon atoms.
Claim 8
Independent Claim
89 words
A method for enhancing the chemiluminescent signal from an acridinium ester encapsulated within a liposome vesicle, in a chemiluminescent reaction which comprises: a) contacting the liposome vesicle with an aqueous solution comprising about 10 to 90% of a first water-miscible alcohol to release the acridinium ester from the liposome vesicle; and b) oxidizing the acridinium ester so released under alkaline pH conditions in the presence of about 10 to 90% of a second water-miscible alcohol wherein the first and second water-miscible alcohols each contain one to three carbon atoms.
Claim 9
Dependent Claim
20 words
A method as recited in claim 6 wherein the first water-miscible alcohol is the same as the second water-miscible alcohol.
Claim 10
Dependent Claim
18 words
A method recited in claim 6 wherein the first and second water-miscible alcohols each contain one hydroxyl group.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-03-03 | 1995-09-02 | 1996-03-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-03-03 | 1999-09-02 | 2000-03-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-03-03 | 2003-09-02 | 2004-03-03 | $7700.00 | Window Closed / Lapsed |