US4640898A
Expired / Lapsed
US
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Homogeneous fluorescence ligang binding assay based upon preferential alteration of the respective intensities of bound and free label by solvent components
Assignee: HEALTH SCIENCES/CHICAGO MEDICAL SCOOL 3333 GREEN BAY ROAD NORTH CHICAGO IL 60064 A NON-PROFIT CORP OF, University of
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Filing: 1983-08-01
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Granted: 1987-02-03
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Expires: 2003-08-01
Abstract
Abstract Fluorescence ligand binding assay of a sample containing an unknown amount of ligand may be performed by making direct intensity measurements. In an immunoassay, for example, the sample may be added to a solution containing fluorescein labeled analyte and then is added an antibody specific to the analyte. Sodium dodecyl sulfate, a surfactant, added to the solution in an amount sufficient to form micelles provides markedly different fluorescent intensity from bound and unbound labeled analyte.
Inventors
- 👤 Clarke J. Halfman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 02:36
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/542; G01N33/58; G01N33/582; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/826; Y10S436/829
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
105 words
A method for conducting a ligand binding assay to determine the concentration of an analyte in a sample containing an unknown amount of the analyte comprising the steps of: preparing an aqueous solution containing the sample, a conjugate comprising the analyte and the fluorescent dye fluorescein, a protein that binds specifically and strongly to the analyte, and the surfactant, sodium dodecyle sulphate, having a micelle threshold, the surfactant concentration being greater than said micelle threshold; and measuring the intensity of fluorescent emission from said solution while irradiating said solution with electromagnetic radiation; said surfactant effecting different fluorescence intensities from bound conjugate and from free conjugate.
Claim 2
Independent Claim
105 words
A method for conducting a ligand binding assay to determine the concentration of an analyte in a sample containing an unknown amount of the analyte comprising the steps of: preparing an aqueous solution containing the sample, a conjugate comprising the analyte and the fluorescent dye fluorescein, a protein that binds specifically and strongly to the analyte, and the surfactant, sodium dodecyle sulphate, having a micelle threshold, the surfactant concentration being greater than said micelle threshold; and measuring the intensity of fluorescent emission from said solution while irradiating said solution with electromagnetic radiation; said surfactant effecting different fluorescence intensities from bound conjugate and from free conjugate.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-02-02 | 1990-08-04 | 1991-02-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-02-02 | 1994-08-04 | 1995-02-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-02-02 | 1998-08-04 | 1999-02-03 | $7700.00 | Window Closed / Lapsed |