US4495293A
Expired / Lapsed
US
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Fluorometric assay
Assignee: Abbott Laboratories
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Filing: 1983-02-24
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Granted: 1985-01-22
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Expires: 2003-02-24
Abstract
Abstract A method is provided to fluorometrically determine a ligand in an assay solution containing the ligand, reagent system and a fluorescer wherein the intensity of the fluorescer emitted by the assay solution is related to the change in the transmittive properties of the assay solution produced by the interaction of the ligand to be determined and a reagent system capable of producing a change in the transmittive properties of the assay solution in the presence of the ligand. In addition, novel reagent compositions are provided which may be utilized to either spectrophotometrically or fluorometrically determine the concentration of a ligand in an assay solution.
Inventors
- 👤 Mark R. Shaffar
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 15:51
CPC Classifications
C; C12; C12Q; C12Q1/00; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/53; G01N33/536; G01N33/542; G01N33/58; G01N33/582; Y; Y10; Y10S; Y10S436/00; Y10S436/80
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
148 words
A method for determining a ligand in a sample suspected of containing the ligand, said method comprising: (a) combining to form an assay solution (i) said sample; (ii) an effective amount of a fluorescer which does not react chemically or immunologically with the ligand; (iii) an effective amount of a reagent system which does not react either chemically or immunogically with the fluorescer and which in the presence of the ligand to be determined is capable of providing a change in the transmittive properties of the assay solution within a wavelength band that overlaps the excitation and/or emission wavelength band of the fluorescer and (b) irradiating the assay solution with light having a wavelength within the excitation wavelength band of the fluorescer; and then (c) measuring the intensity of the fluorescence emitted by the assay solution as a measure of the concentration of the ligand in the sample.
Claim 2
Independent Claim
148 words
A method for determining a ligand in a sample suspected of containing the ligand, said method comprising: (a) combining to form an assay solution (i) said sample; (ii) an effective amount of a fluorescer which does not react chemically or immunologically with the ligand; (iii) an effective amount of a reagent system which does not react either chemically or immunogically with the fluorescer and which in the presence of the ligand to be determined is capable of providing a change in the transmittive properties of the assay solution within a wavelength band that overlaps the excitation and/or emission wavelength band of the fluorescer and (b) irradiating the assay solution with light having a wavelength within the excitation wavelength band of the fluorescer; and then (c) measuring the intensity of the fluorescence emitted by the assay solution as a measure of the concentration of the ligand in the sample.
Claim 3
Dependent Claim
20 words
A method according to claim 1 wherein the reagent system is a chromogenic reagent system or a turbidimetric reagent system.
Claim 4
Dependent Claim
15 words
A method according to claim 2 wherein the reagent system is a chromogenic reagent system.
Claim 5
Dependent Claim
31 words
A method according to claim 3 wherein the absorption wavelength band associated with the change in the transmittive properties of the assay solution overlaps the excitation wavelength band of the fluorescer.
Claim 6
Dependent Claim
31 words
A method according to claim 3 wherein the absorption wavelength band associated with the change in the transmittive properties of the assay solution overlaps the emission wavelength band of the fluorescer.
Claim 7
Dependent Claim
33 words
A method according to claim 3 wherein the absorption wavelength band associated with the change in the transmittive properties of the assay solution overlaps the excitation and emission wavelength bands of the fluorescer.
Claim 8
Dependent Claim
154 words
An improved fluorimetric assay method for determining a ligand in an assay solution containing a sample suspected of containing the ligand, and an effective amount of a reagent system which in the presence of the ligand is capable of providing a change in the transmittive properties of the assay solution within a wavelength band, said improvement comprising adding to the assay solution an effective amount of a fluorescer wherein the fluorescer does not react either chemically or immunologically with the ligand or the reagent system, and wherein the excitation and/or emission wavelength band of the fluorescer overlaps the wavelength band associated with the change in the transmittive properties of the assay solution; irradiating the assay solution with light having a wavelength within the excitation wavelength band of the fluorescer; and then measuring the intensity of the fluorescer emitted by the assay solution as a measure of the concentration of the ligand in the sample.
Claim 9
Dependent Claim
154 words
An improved fluorimetric assay method for determining a ligand in an assay solution containing a sample suspected of containing the ligand, and an effective amount of a reagent system which in the presence of the ligand is capable of providing a change in the transmittive properties of the assay solution within a wavelength band, said improvement comprising adding to the assay solution an effective amount of a fluorescer wherein the fluorescer does not react either chemically or immunologically with the ligand or the reagent system, and wherein the excitation and/or emission wavelength band of the fluorescer overlaps the wavelength band associated with the change in the transmittive properties of the assay solution; irradiating the assay solution with light having a wavelength within the excitation wavelength band of the fluorescer; and then measuring the intensity of the fluorescer emitted by the assay solution as a measure of the concentration of the ligand in the sample.
Claim 10
Dependent Claim
20 words
A method according to claim 7 wherein the reagent system is a chromogenic reagent system or a turbidimetric reagent system.
Claim 11
Dependent Claim
15 words
A method according to claim 8 wherein the reagent system is a chromogenic reagent system.
Claim 12
Dependent Claim
32 words
A method according to claim 9 wherein the fluorescer has an excitation wavelength band that overlaps the absorption wavelength band associated with the change in the transmittive properties of the assay solution.
Claim 13
Dependent Claim
32 words
A method according to claim 9 wherein the fluorescer has an emission wavelength band that overlaps the absorption wavelength band associated with the change in the transmittive properties of the assay solution.
Claim 14
Dependent Claim
36 words
A method according to claim 9 wherein the fluorescer has an excitation wavelength band and emission wavelength band that overlap the absorption wavelength band associated with the change in the transmittive properties of the assay solution.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-01-22 | 1988-07-23 | 1989-01-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-01-22 | 1992-07-23 | 1993-01-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-01-22 | 1996-07-23 | 1997-01-22 | $7700.00 | Window Closed / Lapsed |