US4492762A
Expired / Lapsed
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Fluorescent polarization immunoassays
Assignee: Abbott Laboratories
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Filing: 1982-06-30
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Granted: 1985-01-08
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Expires: 2002-06-30
Abstract
Abstract Improvement in fluorescent polarization immunoassays for substances in blood plasma or serum comprising conducting the assays in dilute anionic surfactant solutions which disrupt the fluorescent bilirubin serum albumin complex without disturbing the antibody reaction in the immunoassay. In this manner, background fluorescence in icteric samples is greatly reduced.
Inventors
- 👤 Chao-Huei J. Wang
- 👤 Stephen D. Stroupe
- 👤 Michael E. Jolley
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 21:18
CPC Classifications
C; C07; C07D; C07D405/00; C07D405/02; C07D405/12; C07D311/00; C07D311/02; C07D311/78; C07D311/80; C07D311/82; C07D473/00; C07D473/02; C07D473/04; C07D473/06; C07D473/08; C07J; C07J41/00; C07J41/0005; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; G01N33/58; G01N33/582; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/825
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
52 words
In a fluorescent polarization immunoassay for a substance in blood serum or plasma the improvement comprising: conducting the fluorescent polarization immunoassay in a solution containing effective amounts of an anionic surfactant to disrupt bilirubin serum albumin complex in the sample and thereby reduce background fluorescence of the blood serum or plasma sample.
Claim 2
Dependent Claim
52 words
In a fluorescent polarization immunoassay for a substance in blood serum or plasma the improvement comprising: conducting the fluorescent polarization immunoassay in a solution containing effective amounts of an anionic surfactant to disrupt bilirubin serum albumin complex in the sample and thereby reduce background fluorescence of the blood serum or plasma sample.
Claim 3
Dependent Claim
14 words
The improvement according to claim 1 wherein the anionic detergent is sodium dodecyl sulfate.
Claim 4
Dependent Claim
13 words
The improvement according to claim 1 wherein the anionic detergent is sodium cholate.
Claim 5
Dependent Claim
13 words
The improvement according to claim 1 wherein the detergent is sodium toluene sulfonate.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-01-08 | 1988-07-09 | 1989-01-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-01-08 | 1992-07-09 | 1993-01-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-01-08 | 1996-07-09 | 1997-01-08 | $7700.00 | Window Closed / Lapsed |