US5618716A
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Materials and methods for biosynthesis of serine and serine-related products
Assignee: Wacker Chemie AG
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Filing: 1992-08-17
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Granted: 1997-04-08
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Expires: 2012-08-17
Abstract
Abstract PCT No. PCT/EP92/01873 Sec. 371 Date May 24, 1994 Sec. 102(e) Date May 24, 1994 PCT Filed Aug. 17, 1992 PCT Pub. No. WO93/12235 PCT Pub. Date Jun. 24, 1993Engineered DNA encoding 3-phosphoglycerate dehydrogenate (PGD) has reduced sensitivity to inhibition by serine in comparison to wild-type PGD. The DNA encodes PGD which has at least some level of enzymatic activity useful for biosynthesis, and which retains that activity at higher serine levels than does the (unmodified) wild-type PGD. The PGD has the amino acid sequence of this above-described engineered DNA. Expression vectors contain this engineered DNA and regulatory DNA positioned and oriented for expression of the engineered DNA in a host expression system. Cells contain these expression vectors, and a method for producing serine or a serine-derived product includes culturing such cells.
Inventors
- 👤 Richard P. Burlingame
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 08:19
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0006; C12P; C12P13/00; C12P13/04; C12P13/06
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
67 words
Engineered DNA encoding 3-phosphoglycerate dehydrogenase (PGD) from E. Coli with reduced sensitivity to inhibition by serine in comparison to wild-type PGD; said DNA encoding PGD from E. Coli comprising a C-terminal insertion into a wild-type PGD sequence; wherein said insertion is between VAL 363 and ASN 364 of wild-type PGD of (SEQ ID NO:1) or between ALA 392 and GLN 394 of wild-type PGD (SEQ ID NO:1).
Claim 2
Dependent Claim
67 words
Engineered DNA encoding 3-phosphoglycerate dehydrogenase (PGD) from E. Coli with reduced sensitivity to inhibition by serine in comparison to wild-type PGD; said DNA encoding PGD from E. Coli comprising a C-terminal insertion into a wild-type PGD sequence; wherein said insertion is between VAL 363 and ASN 364 of wild-type PGD of (SEQ ID NO:1) or between ALA 392 and GLN 394 of wild-type PGD (SEQ ID NO:1).
Claim 3
Dependent Claim
26 words
An expression vector comprising the engineered DNA of claim 1 and regulatory DNA positioned and oriented for expressing said engineered DNA in a host expression system.
Claim 4
Dependent Claim
23 words
A cell comprising the engineered DNA of claim 1, and regulatory DNA positioned and oriented to express said engineered DNA in said cell.
Claim 5
Dependent Claim
14 words
The cell of claim 3, in which said cell is deleted for wild-type serA.
Claim 6
Dependent Claim
29 words
A method for producing a product selected from the group consisting of serine and a serine-derived product, comprising culturing a cell according to claim 3; and recovering said product.
Claim 7
Dependent Claim
11 words
The method of claim 5, in which said product is serine.
Claim 8
Dependent Claim
16 words
A 3-phosphoglycerate dehydrogenase having the amino acid sequence encoded by the engineered DNA of claim 1.
Claim 9
Dependent Claim
98 words
Engineered DNA encoding 3-phosphoglycerate dehydrogenase (PGD) from E. coli with reduced sensitivity to inhibition by serine in comparison to wild-type PGD; said DNA encodes PGD from E. coli having an amino acid sequence selected from the group consisting of the following (SEQ ID NO.: 3); (SEQ ID NO.: 4); (SEQ ID NO.: 5); (SEQ ID NO.: 6); (SEQ ID NO.: 7); (SEQ ID NO.: 8); (SEQ ID NO.: 9); (SEQ ID NO.: 10); (SEQ ID NO.: 11); and (SEQ ID NO.: 12) instead of deleted 52 C-terminal amino acids of the wild-type PGD of (SEQ ID NO.: 1).
Claim 10
Dependent Claim
98 words
Engineered DNA encoding 3-phosphoglycerate dehydrogenase (PGD) from E. coli with reduced sensitivity to inhibition by serine in comparison to wild-type PGD; said DNA encodes PGD from E. coli having an amino acid sequence selected from the group consisting of the following (SEQ ID NO.: 3); (SEQ ID NO.: 4); (SEQ ID NO.: 5); (SEQ ID NO.: 6); (SEQ ID NO.: 7); (SEQ ID NO.: 8); (SEQ ID NO.: 9); (SEQ ID NO.: 10); (SEQ ID NO.: 11); and (SEQ ID NO.: 12) instead of deleted 52 C-terminal amino acids of the wild-type PGD of (SEQ ID NO.: 1).
Claim 11
Dependent Claim
16 words
A 3-phosphoglycerate dehydrogenase having the amino acid sequence encoded by the engineered DNA of claim 8.
References Cited (Backward Citations)
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US4753883A
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EP0372962A2
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EP0418840A2
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-04-07 | 2000-10-07 | 2001-04-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-04-07 | 2004-10-07 | 2005-04-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-04-07 | 2008-10-07 | 2009-04-08 | $7700.00 | Window Closed / Lapsed |