EP0418840A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Methods and materials for phe A feedback inhibition resistance
Assignee: Nutrasweet Co
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Filing: 1990-09-19
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Granted: —
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Expires: 2010-09-19
Abstract
Abstract Disclosed are DNA sequences encoding deletion, substitution and/or addition analogs of the E. coli enzyme, chorismate mutase/prephenate dehydratase (CMPD). Preferred expression products include [des-Gln³⁰⁷, des-Ala³⁰⁸, des-Gly³⁰⁹, des-Ala³¹⁰]CMPD; [Leu³⁰⁶]CMPD; [des-Thr³⁰⁴, Lys³⁰⁵, des-Gln³⁰⁶]CMPD; and [Cys³⁰⁹]CMPD display enzymatic activity of the wild type enzyme but are more resistant to inhibition in the presence of phenylalanine.
Inventors
- 👤 Ian Graham Fotheringham
- 👤 Jennifer Nelms
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 08:35
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/52; C07; C07H; C07H21/00; C07H21/02; C12N9/00; C12N9/88; C12P; C12P13/00; C12P13/04; C12P13/22; C12P13/222
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
20 words
DNA sequences encoding deletion analogs, substitution analogs and/or addition analogs, affecting the residues 301 to 315 of E. coli CMPD.
Claim 2
Dependent Claim
20 words
DNA sequences encoding deletion analogs, substitution analogs and/or addition analogs, affecting the residues 301 to 315 of E. coli CMPD.
Claim 3
Dependent Claim
25 words
DNA sequences according to claim 1, encoding the deletion analogs, substitution analogs and/or addition analogs affecting the residues 304 to 310 of E. coli CMPD.
Claim 4
Dependent Claim
13 words
DNA sequence according to claim 2, encoding E. coli [des-Gln³⁰⁷, des-Ala³⁰⁸, des-Gly³⁰⁹, des-Ala³¹⁰]CMPD.
Claim 5
Dependent Claim
10 words
DNA sequence according to claim 2, encoding E. coli [Leu³⁰⁶]CMPD.
Claim 6
Dependent Claim
12 words
DNA squence according to claim 2, encoding E. coli [des-Thr³⁰⁴, Lys³⁰⁵, des-Gln³⁰⁶]CMPD.
Claim 7
Dependent Claim
10 words
DNA sequence according to claim 2, encoding E. coli [Cys³⁰⁹]CMPD.
Claim 8
Dependent Claim
10 words
DNA expression vectors comprising DNA sequences according to claim 1.
Claim 9
Dependent Claim
15 words
DNA expression vectors comprising DNA sequences according to one of the claims 2 through 6.
References Cited (Backward Citations)
No citation records indexed.
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