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US5593838A
Expired / Lapsed
US
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Partitioned microelectronic device array
Assignee: Sarnoff Corp
•
Filing: 1995-05-31
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Granted: 1997-01-14
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Expires: 2015-05-31
Abstract
Abstract A system for processing a plurality of tests or syntheses in parallel comprising a sample channel for moving samples into a microlaboratory array of a plurality of wells connected by one or more channels for the testing or synthesis of samples, a station for housing the array and an optical system comprising at least one light source and at least one light detector for measuring the samples in the array, and a means of electrically connecting said array to an apparatus capable of monitoring and controlling the flow of fluids into the array. Samples are loaded from a common loading channel into the array, processed in the wells and measurements taken by the optical system. The array can process many samples, or synthesize many compounds in parallel, reducing the time required for such processes.
Inventors
- 👤 Peter J. Zanzucchi
- 👤 Satyam C. Cherukuri
- 👤 Sterling E. McBride
- 👤 Amrit K. Judd
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 15:48
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; B; B01; B01L; B01L7/00; B01L7/52; B01J; B01J19/00; B01J19/0046; B01J19/0093; B01L3/00; B01L3/50; B01L3/502; B01L3/5025; B01L3/5027; B01L3/502707; B01L3/50273; B01L3/502738; C; C07; C07K; C07K1/00; C07K1/04; C07K1/042; G01N27/00; G01N27/26; G01N33/543; G01N33/54366; G01N33/558; G01N33/561; G01N35/00; B01F; B01F33/00; B01F33/30; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00279; B01J2219/00306; B01J2219/00313; B01J2219/00315; B01J2219/00317; B01J2219/00351; B01J2219/00353; B01J2219/00364; B01J2219/00367; B01J2219/00369; B01J2219/00389; B01J2219/00479; B01J2219/00488; B01J2219/00495; B01J2219/00497; B01J2219/005; B01J2219/00527; B01J2219/00536; B01J2219/0054; B01J2219/00547; B01J2219/00583; B01J2219/00585; B01J2219/0059; B01J2219/00596; B01J2219/00603; B01J2219/00605; B01J2219/00612; B01J2219/00614; B01J2219/00621; B01J2219/00623; B01J2219/00626; B01J2219/00632; B01J2219/00637; B01J2219/00646; B01J2219/00648; B01J2219/00659; B01J2219/0068; B01J2219/00686; B01J2219/00698; B01J2219/00718; B01J2219/0072; B01J2219/00722; B01J2219/00725; B01J2219/00781; B01J2219/00819; B01J2219/00824; B01J2219/00826; B01J2219/00828; B01J2219/00831; B01J2219/00891; B01J2219/0095; B01J2219/00952; B01L2200/00; B01L2200/06; B01L2200/0605; B01L2200/12; B01L2200/14; B01L2200/143; B01L2200/147; B01L2300/00; B01L2300/08; B01L2300/0803; B01L2300/0806; B01L2300/0861; B01L2300/0864; B01L2300/0867; B01L2300/087; B01L2300/0874; B01L2300/18; B01L2300/1805; B01L2300/1827; B01L2400/00; B01L2400/04; B01L2400/0403; B01L2400/0406; B01L2400/0409; B01L2400/0415; B01L2400/06; B01L2400/0633; B01L3/502715; C40; C40B; C40B40/00; C40B40/04; C40B40/06; C40B40/10; C40B60/00; C40B60/14; C40B70/00; Y; Y10; Y10S; Y10S436/00; Y10S436/805; Y10S436/807; Y10T; Y10T436/00; Y10T436/11
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
189 words
A method of assaying nucleic acid which comprises separately and in parallel for each of two or more nucleic acid-containing samples: placing a first nucleic acid-containing sample and a second nucleic-acid-containing sample into an array comprising a solid substrate, a plurality of wells and channels, wherein at least one channel is formed in the upper surface of the substrate and is situated above another channel formed in the lower surface of the substrate, so as to form crossovers comprising crossing but non-intersecting channels; moving said first sample into a first well for isolating nucleic acid; moving said second sample into a second well for isolating nucleic acid; moving the isolated nucleic acid from the first sample to a third well; moving the isolated nucleic acid from the second sample to a fourth well; amplifying the nucleic acid in the third and fourth wells by cycling the temperature of the third and fourth wells; moving the amplified nucleic acid in the third well to a fifth well for assay; moving the amplified nucleic acid in the fourth well to a sixth well for assay; and assaying the amplified nucleic acid.
Claim 2
Independent Claim
189 words
A method of assaying nucleic acid which comprises separately and in parallel for each of two or more nucleic acid-containing samples: placing a first nucleic acid-containing sample and a second nucleic-acid-containing sample into an array comprising a solid substrate, a plurality of wells and channels, wherein at least one channel is formed in the upper surface of the substrate and is situated above another channel formed in the lower surface of the substrate, so as to form crossovers comprising crossing but non-intersecting channels; moving said first sample into a first well for isolating nucleic acid; moving said second sample into a second well for isolating nucleic acid; moving the isolated nucleic acid from the first sample to a third well; moving the isolated nucleic acid from the second sample to a fourth well; amplifying the nucleic acid in the third and fourth wells by cycling the temperature of the third and fourth wells; moving the amplified nucleic acid in the third well to a fifth well for assay; moving the amplified nucleic acid in the fourth well to a sixth well for assay; and assaying the amplified nucleic acid.
Claim 3
Dependent Claim
29 words
A method according to claim 1 wherein a red corpuscle buffer solution and a white corpuscle buffer solution are moved sequentially into the first well and the second well.
Claim 4
Dependent Claim
16 words
A method according to claim 1 wherein the first well and the second well are heated.
Claim 5
Dependent Claim
11 words
The method of claim 1, further comprising assaying by optical detection.
Claim 6
Independent Claim
111 words
A method of assaying nucleic acid which comprises separately and in parallel for each of two or more nucleic acid-containing samples: placing a first nucleic acid-containing sample and a second nucleic acid-containing sample into an array comprising a solid substrate, a plurality of wells and channels, wherein at least one channel is formed in the upper surface of the substrate and is situated above another channel formed in the lower surface of the substrate, so as to form crossovers comprising crossing but non-intersecting channels; separately isolating nucleic acid from the first and second sample; separately amplifying the nucleic acid from the first and second sample; and assaying the amplified nucleic acid.
Claim 7
Independent Claim
111 words
A method of assaying nucleic acid which comprises separately and in parallel for each of two or more nucleic acid-containing samples: placing a first nucleic acid-containing sample and a second nucleic acid-containing sample into an array comprising a solid substrate, a plurality of wells and channels, wherein at least one channel is formed in the upper surface of the substrate and is situated above another channel formed in the lower surface of the substrate, so as to form crossovers comprising crossing but non-intersecting channels; separately isolating nucleic acid from the first and second sample; separately amplifying the nucleic acid from the first and second sample; and assaying the amplified nucleic acid.
Claim 8
Dependent Claim
11 words
The method of claim 5, further comprising assaying by optical detection.
Claim 9
Dependent Claim
18 words
The method according to claim 5 wherein the nucleic acid is amplified using the polymerase chain reaction method.
Claim 10
Dependent Claim
16 words
The method according to claim 5 wherein the nucleic acid is assayed using probe hybridization techniques.
Claim 11
Dependent Claim
25 words
The method according to claim 5 wherein the isolation of nucleic acid is achieved by treating the sample with a lysis solution and a proteinase.
Claim 12
Dependent Claim
37 words
The method according to claim 5 wherein the substrate has at least one well for each sample for isolating nucleic acid, another well for amplifying nucleic acid, and a further well for assaying the amplified nucleic acid.
Claim 13
Dependent Claim
25 words
The method according to claim 5 wherein the substrate further comprises DNA binding paramagnetic beads that fit into one or more wells of the substrate.
Claim 14
Dependent Claim
57 words
The method according to claim 5 wherein the substrate has a first cover that is sealed onto the top of the substrate to enclose said channel in the upper surface of the substrate and a second cover that is sealed to the bottom of the substrate to enclose said channel in the lower surface of the substrate.
Claim 15
Dependent Claim
23 words
The method according to claim 5 wherein the substrate further comprises a control means to control fluid flow into a channel or well.
Claim 16
Dependent Claim
16 words
The method according to claim 5 wherein the substrate further comprises reservoirs comprising plastic release containers.
Claim 17
Dependent Claim
12 words
The method according to claim 14 wherein the plastic containers contain primers.
Claim 18
Dependent Claim
22 words
The method according to claim 5 wherein the first sample and the second sample are treated sequentially in a series of wells.
Claim 19
Dependent Claim
27 words
The method according to claim 16 wherein the nucleic acid for the first and second samples are isolated in a first well and a second well, respectively.
Claim 20
Dependent Claim
27 words
The method according to claim 17 wherein the nucleic acid for the first and second samples are amplified in a third well and a fourth well, respectively.
Claim 21
Dependent Claim
27 words
The method according to claim 18 wherein the nucleic acid for the first and second samples are assayed in a fifth well and a sixth well, respectively.
Claim 22
Dependent Claim
11 words
The method according to claim 5 wherein the sample comprises blood.
Claim 23
Dependent Claim
23 words
The method according to claim 5 wherein the substrate comprises reservoirs in which reagents or solvents are stored or built into the substrate.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-01-14 | 2000-07-15 | 2001-01-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-01-14 | 2004-07-15 | 2005-01-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-01-14 | 2008-07-15 | 2009-01-14 | $7700.00 | Window Closed / Lapsed |