US4385115A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Diagnostics testing devices and processes
Assignee: Hoffmann La Roche Inc
•
Filing: 1980-10-22
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Granted: 1983-05-24
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Expires: 2000-10-22
Abstract
Abstract A convenient, self-contained, disposable device suitable for the rapid identification of microorganisms, having capability for simultaneously carrying out a multiplicity of analytical determinations. The device comprises a disc-shaped arrangement defining a multiplicity of satellite testing chambers each of which is connected to a centrally defined chamber. The central chamber is provided with a movable sleeve or piston arrangement. Liquid sample is received in the central chamber and the sleeve is manipulated to provide samples uniformly and simultaneously to each of the satellite testing chambers without resorting to external means, such as positive or a negative pressurization or centrifugal force. Also disclosed are unique disc-shaped optically transparent support films which are coated on one or both sides with analytical substrates or reagents which are predispensed to the satellite testing chambers.
Inventors
- 👤 Edward F. de Zabala
- 👤 Edward V. Savard
- 👤 Doris B. Taylor
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 14:30
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; B; B01; B01L; B01L3/00; B01L3/50; B01L3/502; C12M; C12M41/00; C12M41/30; C12M41/34; C12M41/36; G; G01; G01N; G01N21/00; G01N21/01; G01N21/03; G01N21/17; G01N21/25; G01N21/251; G01N21/253; B01L2200/00; B01L2200/06; B01L2200/0605; B01L2200/0684; B01L2300/00; B01L2300/08; B01L2300/0803; B01L2300/0861; B01L2300/0864; B01L2400/00; B01L2400/04; B01L2400/0403; B01L2400/0409; B01L2400/0475; B01L2400/0478; G01N2021/0325; G01N2021/0328; G01N21/75; G01N21/77; G01N21/78
Claims Analysis
Total Claims: 35
Claim 1
Independent Claim
168 words
A diagnostic device having a first chamber for receiving a sample and a multiplicity of separate analysis chambers in controlled communication only with the first chamber, wherein the improvement comprises the combination of: (a) at least one diagnostically useful substance predispensed in solid form to each of the individually viewable, optically transparent analysis chambers; (b) optically transparent and flat cover seal means being disposed above each of the analysis chambers to render each analysis chamber individually viewable in a vertical orientation from above said analysis chamber; (c) self-contained means for controlling communication between the first source chamber and the optically transparent analysis chambers, and for dispensing sample from the first chamber simultaneously and in uniform amount to each of the analysis chambers concurrently with venting said first chamber said means being adjustably interdisposed between said first chamber and said analysis chambers; and (d) analysis chambers circumferentially arranged relative to the first chamber, each of said analysis chambers sharing in common a wall with the immediately adjacent analysis chambers.
Claim 2
Dependent Claim
168 words
A diagnostic device having a first chamber for receiving a sample and a multiplicity of separate analysis chambers in controlled communication only with the first chamber, wherein the improvement comprises the combination of: (a) at least one diagnostically useful substance predispensed in solid form to each of the individually viewable, optically transparent analysis chambers; (b) optically transparent and flat cover seal means being disposed above each of the analysis chambers to render each analysis chamber individually viewable in a vertical orientation from above said analysis chamber; (c) self-contained means for controlling communication between the first source chamber and the optically transparent analysis chambers, and for dispensing sample from the first chamber simultaneously and in uniform amount to each of the analysis chambers concurrently with venting said first chamber said means being adjustably interdisposed between said first chamber and said analysis chambers; and (d) analysis chambers circumferentially arranged relative to the first chamber, each of said analysis chambers sharing in common a wall with the immediately adjacent analysis chambers.
Claim 3
Dependent Claim
21 words
A device according to claim 1 wherein said diagnostically useful substance is disposed apart from a wall defining said analysis chamber.
Claim 4
Dependent Claim
26 words
A device according to claim 1 or 2 wherein said at least one diagnostically useful substance comprises a pair of such substances which are normally incompatible.
Claim 5
Dependent Claim
25 words
A device according to claim 1 or 2 wherein said diagnostically useful substance is coated onto at least one surface of an optically transparent support.
Claim 6
Dependent Claim
52 words
A device according to claim 4 wherein at least one diagnostically useful substance is coated onto each side of said support, and at least one of said substances on the one side of said support is normally incompatible with at least one of said substances on the other side of said support.
Claim 7
Dependent Claim
19 words
A device according to claim 5 wherein each substance coated onto said support has a property for microorganism identification.
Claim 8
Dependent Claim
37 words
A device according to claim 5 wherein said analysis locations are provided with means for supporting a side of said optically transparent support thereby enabling simultaneous access of the sample to all surfaces of said predispensed substances.
Claim 9
Dependent Claim
30 words
A device according to claim 1 wherein the optically transparent analysis chambers are circumferentially arranged relative to the first chamber and share in common an optically transparent cover seal means.
Claim 10
Dependent Claim
52 words
A device according to claim 1 wherein said self-contained accessing means is adjustably interdisposed between said source and analysis locations and is structured for a reversible linear vertical movement relative to source and analysis locations whereby communication between said source and analysis locations and venting above said source location is concurrently controlled.
Claim 11
Dependent Claim
31 words
A device according to claim 9 wherein said self-contained accessing means is a substantially cylindrical sleeve disposed adjacent to and surrounding said source location and structured to provide a vertical displacement.
Claim 12
Dependent Claim
51 words
A device according to claim 1 wherein said self-contained accessing means is adjustably interdisposed between said source and analysis locations and is structured for a reversible rotary movement relative to source and analysis locations whereby communication between said source and analysis locations and venting above said source location is concurrently controlled.
Claim 13
Dependent Claim
32 words
A device according to claim 11 wherein said self-contained accessing means is a substantially cylindrical sleeve disposed adjacent to and surrounding said source location and structured to provide a horizontal rotary movement.
Claim 14
Dependent Claim
45 words
A device according to claim 1 wherein said self-contained accessing means is adjustably interdisposed between said source and analysis locations and is structured for a reversible screw movement whereby communication between said source and analysis locations and venting above said source location is concurrently controlled.
Claim 15
Independent Claim
45 words
A device according to any of claims 13 wherein said self-contained accessing means provides means for simultaneously closing the vent means above the first chamber and blocking communication between the source and analysis locations thereby sealing the dispensed sample at each of the analysis locations.
Claim 16
Independent Claim
29 words
A device according to any of claims 9, 13 or 14 wherein said self-contained accessing means comprises a substantially cylindrical sleeve disposed adjacent to and surrounding said source location.
Claim 17
Dependent Claim
37 words
A device according to claim 15 wherein said source location comprises a first chamber and said analysis locations comprise a multiplicity of separate, optically transparent analysis chambers circumferentially spaced about the substantially cylindrical sleeve and source chamber.
Claim 18
Dependent Claim
74 words
A device according to claim 16 further comprising a multiplicity of passageway means for facilitating transfer of sample from the first chamber to the optically transparent analysis chambers when said sleeve is moved, said multiplicity of passageway means being formed on the wall defining the first chamber and radiating out to the analysis chambers from said first chamber and each said passageway means being in communication with a an optically transparent, separate analysis chamber.
Claim 19
Dependent Claim
20 words
A device according to claim 16 wherein each of the analysis locations includes a venting arrangement above the analysis locations.
Claim 20
Dependent Claim
14 words
A device according to claim 18 wherein said venting arrangement includes a venting chamber.
Claim 21
Dependent Claim
28 words
A device according to claim 19 wherein said venting arrangement further includes means for enabling the venting chamber to communicate with the respective analysis chamber and the atmosphere.
Claim 22
Dependent Claim
24 words
A device according to claim 20 wherein said enabling means is structured to pass gaseous matter and block passage of liquid and solid matter.
Claim 23
Dependent Claim
16 words
A device according to claim 19 wherein said venting chamber is disposed above the analysis chamber.
Claim 24
Dependent Claim
22 words
A device according to claim 19 wherein said venting chamber is provided with at least one predispensed substance useful in sample identification.
Claim 25
Dependent Claim
36 words
A device according to claim 1 wherein said self-contained accessing means comprises a piston arrangement adjustably disposed at the source location for pressurizing the first chamber whereby communication between the source and analysis locations is controlled.
Claim 26
Dependent Claim
20 words
A device according to claim 24 wherein said self-contained accessing means further comprises a screw movement adjustable, threaded piston arrangement.
Claim 27
Dependent Claim
35 words
A device according to claim 1 further including a multiplicity of inclined passageway means in controlled communication with the source location and the analysis locations for facilitating the dispensing of sample to the analysis locations.
Claim 28
Dependent Claim
20 words
A device according to claim 1 wherein a plurality of said substances is predispensed in at least one analysis location.
Claim 29
Independent Claim
18 words
A device according to any one of claims 1, and 27 wherein said substances are in solid form.
Claim 30
Dependent Claim
22 words
A device according to claim 28 wherein said substances are coated on opposite sides of a support material which is optically transparent.
Claim 31
Dependent Claim
21 words
A device according to claim 29 wherein at least one analysis location is provided with a plurality of said coated supports.
Claim 32
Dependent Claim
127 words
Diagnostic method comprising: (a) predepositing in solid form at least one selected substance useful in the identification of at least a part of a sample, into each of a series of separate, optically transparent analysis chambers; (b) depositing a sample in a single source chamber; (c) providing a portion of said sample simultaneously to each of the separate analysis chambers and while maintaining the orientation of the device concurrently venting said source chamber, such that a substantially uniform amount of sample is received therein; and (d) individually measuring the nature and extent of reactions between the sample portion and substance useful in its identification in each optically transparent, separate analysis chamber whereby the nature and extent of said reactions are indicative of identifying characteristics of said sample.
Claim 33
Dependent Claim
127 words
Diagnostic method comprising: (a) predepositing in solid form at least one selected substance useful in the identification of at least a part of a sample, into each of a series of separate, optically transparent analysis chambers; (b) depositing a sample in a single source chamber; (c) providing a portion of said sample simultaneously to each of the separate analysis chambers and while maintaining the orientation of the device concurrently venting said source chamber, such that a substantially uniform amount of sample is received therein; and (d) individually measuring the nature and extent of reactions between the sample portion and substance useful in its identification in each optically transparent, separate analysis chamber whereby the nature and extent of said reactions are indicative of identifying characteristics of said sample.
Claim 34
Dependent Claim
13 words
A method according to claim 31 wherein said sample identification comprises identifying microorganisms.
Claim 35
Dependent Claim
15 words
A method according to claim 32 wherein said microorganism identification includes measuring the endogenous respiration.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-05-23 | 1986-11-22 | 1987-05-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-05-23 | 1990-11-22 | 1991-05-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-05-23 | 1994-11-22 | 1995-05-24 | $7700.00 | Window Closed / Lapsed |