US5591625A
Expired / Lapsed
US
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Transduced mesenchymal stem cells
Assignee: Case Western Reserve University
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Filing: 1993-11-24
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Granted: 1997-01-07
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Expires: 2013-11-24
Abstract
Abstract Genetically engineered human stem cells that carry within them genes of interest particularly for the expression of physiologically or pharmacologically active proteins or for use in gene therapy. In addition to correction of genetic disorders, is the ability to introduce, in a targeted manner, additional copies of essential genes to allow expression in proliferating, nondifferentiating cells of certain gene products. These genes can be, for example, hormones matrix proteins, cytokines, adhesion molecules, detoxification enzymes and "rebuilding" proteins important in tissue repair.
Inventors
- 👤 Stanton L. Gerson
- 👤 Arnold I. Caplan
- 👤 Stephen E. Haynesworth
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 06:59
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/86; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/13011; C12N2740/13041; C12N2740/13043
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
26 words
Isolated human mesenchymal stem cells which can differentiate into more than one connective tissue type transfected with exogenous genetic material encoding a protein to be expressed.
Claim 2
Dependent Claim
26 words
Isolated human mesenchymal stem cells which can differentiate into more than one connective tissue type transfected with exogenous genetic material encoding a protein to be expressed.
Claim 3
Dependent Claim
15 words
The isolated human mesenchymal stem cells of claim 1 that are non-embryonic mesenchymal stem cells.
Claim 4
Dependent Claim
15 words
The isolated human mesenchymal stem cells of claim 1 that are marrow-derived mesenchymal stem cells.
Claim 5
Dependent Claim
27 words
The isolated human mesenchymal stem cells of claim 1 that have been transfected with a DNA sequence which codes for at least one protein to be expressed.
Claim 6
Dependent Claim
42 words
The isolated human mesenchymal stem cells of claim 4 that have been transfected with a DNA sequence comprising a retroviral 5' LTR and at least one of a retroviral gag, pol or env gene under the transcriptional control of said 5' LTR.
Claim 7
Dependent Claim
24 words
The isolated human mesenchymal stem cells of claim 5 that have also been transfected with a DNA sequence comprising a retroviral packaging signal sequence.
Claim 8
Dependent Claim
32 words
The isolated human mesenchymal stem cells of claim 1 which include a cell surface epitope specifically bound by antibodies from hybridoma cell line SH2, deposited with the ATCC under accession number HB10743.
Claim 9
Dependent Claim
33 words
The isolated human mesenchymal stem cells of claim 7 which further include a cell surface epitope specifically bound by antibodies from hybridoma cell line SH3, deposited with the ATCC under accession number HB10744.
Claim 10
Dependent Claim
32 words
The isolated human mesenchymal stem cells of claim 7 which include a cell surface epitope specifically bound by antibodies from hybridoma cell line SH4, deposited with the ATCC under accession number HB10745.
Claim 11
Dependent Claim
26 words
A composition comprising the isolated human mesenchymal stem cells of claim 1 in a medium that stimulates their culture expansion but does not stimulate their differentiation.
Claim 12
Dependent Claim
12 words
The composition of claim 10 wherein the medium comprises a supplemented DMEM.
Claim 13
Dependent Claim
10 words
A composition of claim 11 wherein the medium comprises serum.
Claim 14
Dependent Claim
12 words
The composition of claim 12 wherein the medium comprises fetal animal serum.
Claim 15
Dependent Claim
14 words
The composition of claim 11 which is supplemented with an antibiotic and antimycotic composition.
Claim 16
Dependent Claim
17 words
A composition comprising the isolated human mesenchymal stem cells of claim 1 in supplemented BGJ b medium.
Claim 17
Dependent Claim
17 words
A composition comprising the isolated human mesenchymal stem cells of claim 1 in supplemented F-12 Nutrient Mixture.
Claim 18
Dependent Claim
38 words
The composition of claim 1 wherein the isolated human mesenchymal stem cells of claim 1 are isolated from other cells by selective binding with antibodies from hybridoma cell line SH2, deposited with the ATCC under accession number HB10743.
Claim 19
Dependent Claim
19 words
The composition of claim 10 wherein the mesenchymal stem cells are isolated from other cells by density gradient fractionation.
Claim 20
Dependent Claim
19 words
The composition of claim 10 wherein the mesenchymal stem cells are isolated from other cells by Percoll gradient fractionation.
Claim 21
Dependent Claim
30 words
The isolated human mesenchymal stem cells of claim 4 that have been transduced with a retroviral vector that includes the DNA sequence that codes for the protein to be expressed.
Claim 22
Dependent Claim
23 words
The isolated human mesenchymal stem cells of claim 20 wherein transcription of the DNA sequence is under the control of a retroviral LTR.
References Cited (Backward Citations)
US3968567A
Cited
US4254226A
Cited
US4430760A
Cited
US4642120A
Cited
US4714680A
Cited
US4714680B1
Cited
US4609551A
Cited
US4596574A
Cited
WO1987000201A1
Cited
US4721096A
Cited
EP0243204A2
Cited
WO1988008306A1
Cited
WO1989002468A1
Cited
WO1989005345A1
Cited
WO1989007136A2
Cited
US4904259A
Cited
WO1990002806A1
Cited
WO1990010872A1
Cited
WO1990011359A1
Cited
US5399346A
Cited
WO1992007573A1
Cited
WO1992007943A1
Cited
US5226914A
Cited
US5197985A
Cited
US5486359A
Cited
WO1992012242A1
Cited
US5206023A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-01-07 | 2000-07-08 | 2001-01-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-01-07 | 2004-07-08 | 2005-01-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-01-07 | 2008-07-08 | 2009-01-07 | $7700.00 | Window Closed / Lapsed |