US4714680A
Expired / Lapsed
US
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Human stem cells
Assignee: Johns Hopkins University
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Filing: 1984-02-06
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Granted: 1987-12-22
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Expires: 2004-02-06
Abstract
Abstract Monoclonal antibodies that recognize a stage-specific antigen on immature human marrow cells are provided. These antibodies are useful in methods of isolating cell suspensions from human blood and marrow that can be employed in bone marrow transplantation. Cell suspensions containing human pluripotent lympho-hematopoietic stem cells are also provided, as well as theraputic methods employing the cell suspensions.
Inventors
- 👤 Curt I. Civin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 12:29
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/28; C; C07; C07K; C07K16/00; C07K16/18; C07K16/28
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
18 words
A suspension of human cells comprising pluripotent lympho-hematopoietic stem cells substantially free of mature lymphoid and myeloid cells.
Claim 2
Dependent Claim
18 words
A suspension of human cells comprising pluripotent lympho-hematopoietic stem cells substantially free of mature lymphoid and myeloid cells.
Claim 3
Dependent Claim
32 words
The cell suspension of claim 1 further comprising colony-forming cells for granulocytes/monocytes (CC-GM), colony-forming cells for erythrocytes (BFU-E), colony-forming cells for eosinophils (CFC-Eo), multipotent colony-forming cells (CFC-GEMM), and immature lymphoid precursor cells.
Claim 4
Dependent Claim
29 words
The cell suspension of claim 1 substantially free of cells without a cell-surface antigen recognized by the monoclonal antibody produced by the hybridoma deposited under ATCC Accession No. HB-8483.
Claim 5
Dependent Claim
20 words
A suspension of human cells from blood comprising pluripotent lympho-hematopoietic stem cells substantially free of mature lymphoid and myeloid cells.
Claim 6
Dependent Claim
20 words
A suspension of human cells from blood comprising pluripotent lympho-hematopoietic stem cells substantially free of mature lymphoid and myeloid cells.
Claim 7
Dependent Claim
65 words
A suspension of human cells from marrow or blood comprising cells having a cell-surface antigen recognized by the antibody produced by the hybridoma deposited under ATCC Accession No. HB-8483 and substantially free of cells that do not have a cell-surface antigen recognized by said antibody, said suspension having the ability to restore the production of lymphold and hematopoietic cells to a human lacking said production.
Claim 8
Dependent Claim
65 words
A suspension of human cells from marrow or blood comprising cells having a cell-surface antigen recognized by the antibody produced by the hybridoma deposited under ATCC Accession No. HB-8483 and substantially free of cells that do not have a cell-surface antigen recognized by said antibody, said suspension having the ability to restore the production of lymphold and hematopoietic cells to a human lacking said production.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-12-21 | 1991-06-22 | 1991-12-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-12-21 | 1995-06-22 | 1995-12-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-12-21 | 1999-06-22 | 1999-12-22 | $7700.00 | Window Closed / Lapsed |