US5567301A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Antibody covalently bound film immunobiosensor
Assignee: Illinois Institute of Technology
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Filing: 1995-03-01
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Granted: 1996-10-22
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Expires: 2015-03-01
Abstract
Abstract A biosensor comprising a substrate material, at least one antibody covalently immobilized on the substrate material, and a pair of metal contact electrodes for measuring the impedance of the biosensor. A process for producing a biosensor in accordance with this invention as well as a process for utilizing the biosensor are also disclosed.
Inventors
- 👤 Joseph R. Stetter
- 👤 Peter J. Hesketh
- 👤 Steven M. Gendel
- 👤 G. Jordan Maclay
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 03:40
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
28 words
A biosensor comprising: a substrate material; at least one covalently bound antibody immobilized on said substrate material; and impedance detection means for measuring an impedance of said biosensor.
Claim 2
Dependent Claim
28 words
A biosensor comprising: a substrate material; at least one covalently bound antibody immobilized on said substrate material; and impedance detection means for measuring an impedance of said biosensor.
Claim 3
Dependent Claim
25 words
A biosensor in accordance with claim 1, wherein said substrate material is a material capable of being processed by at least one semiconductor device process.
Claim 4
Dependent Claim
16 words
A biosensor in accordance with claim 2, wherein said substrate material is a planar semiconductor device.
Claim 5
Dependent Claim
20 words
A biosensor in accordance with claim 3, wherein said substrate material comprises an insulator layer passivated on a silicon chip.
Claim 6
Dependent Claim
14 words
A biosensor in accordance with claim 4, wherein said insulator layer is silicon dioxide.
Claim 7
Dependent Claim
20 words
A biosensor in accordance with claim 4 further comprising a discontinuous ultra-thin metal film layer deposited on said insulator layer.
Claim 8
Dependent Claim
35 words
A biosensor in accordance with claim 6, wherein said discontinuous ultra-thin metal film layer comprises a plurality of distributed islands of metal with an uncovered portion of said insulator layer disposed between neighboring said islands.
Claim 9
Dependent Claim
35 words
A biosensor in accordance with claim 7, wherein a size of said uncovered portion of said insulator layer between said neighboring islands corresponds to a size range of said at least one covalently bound antibody.
Claim 10
Dependent Claim
26 words
A biosensor in accordance with claim 6, wherein said metal is selected from the group consisting of platinum, palladium, gold, iridium, rhodium, osmium and mixtures thereof.
Claim 11
Dependent Claim
24 words
A biosensor in accordance with claim 6, wherein said at least one covalently bound antibody is disposed on said discontinuous ultra-thin metal film layer.
Claim 12
Dependent Claim
25 words
A biosensor in accordance with claim 6, wherein said impedance detection means comprises two metal contact electrodes disposed on said discontinuous ultra-thin metal film layer.
Claim 13
Dependent Claim
27 words
A biosensor in accordance with claim 4, wherein said impedance detection means comprises two metal contact electrodes disposed on said insulator at a distance from one another.
Claim 14
Dependent Claim
31 words
A biosensor in accordance with claim 12, wherein said at least one covalently bound antibody is disposed on at least one of said insulator layer and said two metal contact electrodes.
Claim 15
Dependent Claim
64 words
A process for producing a biosensor for measuring antigen-antibody binding reactions comprising: thermally growing an insulator layer on a silicon chip substrate; depositing a discontinuous ultra-thin metal film layer on said insulator; securing two metal contact-electrodes at a distance from each other on said discontinuous ultra-thin metal film layer; and covalently immobilizing at least one antibody bioactive layer on said discontinuous ultra-thin metal layer.
Claim 16
Dependent Claim
64 words
A process for producing a biosensor for measuring antigen-antibody binding reactions comprising: thermally growing an insulator layer on a silicon chip substrate; depositing a discontinuous ultra-thin metal film layer on said insulator; securing two metal contact-electrodes at a distance from each other on said discontinuous ultra-thin metal film layer; and covalently immobilizing at least one antibody bioactive layer on said discontinuous ultra-thin metal layer.
Claim 17
Dependent Claim
36 words
A process in accordance with claim 14, wherein said insulator layer is a silicon dioxide layer thermally grown in a dry oxygen atmosphere at a temperature in the range of about 900° C. to 1300° C.
Claim 18
Dependent Claim
26 words
A process in accordance with claim 14, wherein said metal is selected from the group consisting of platinum, palladium, gold, iridium, rhodium, osmium and mixtures thereof.
Claim 19
Dependent Claim
12 words
A process in accordance with claim 16, wherein said metal is platinum.
Claim 20
Dependent Claim
35 words
A process in accordance with claim 14, wherein said discontinuous ultra-thin metal film layer comprises a plurality of distributed islands of metal with an uncovered portion of said insulator layer disposed between neighboring said islands.
Claim 21
Dependent Claim
27 words
A process in accordance with claim 14, wherein said discontinuous ultra-thin metal film layer is electrically deposited on said insulator layer by one of sputtering and evaporation.
Claim 22
Dependent Claim
15 words
A process in accordance with claim 14, wherein said at least one antibody is anti-SEB.
Claim 23
Dependent Claim
45 words
A process in accordance with claim 20, wherein said anti-SEB layer is immobilized by silanization of the surface of said discontinuous ultra-thin metal film layer followed by reaction of said silanized said discontinuous ultra-thin metal film layer with a crosslinking agent and then said anti-SEB.
Claim 24
Dependent Claim
25 words
A process in accordance with claim 21, wherein said anti-SEB is covalently bound to said silanized discontinuous ultra-thin metal film layer by a chemical bond.
Claim 25
Dependent Claim
25 words
A process in accordance with claim 22, wherein said anti-SEB is covalently bound to said silanized discontinuous ultra-thin metal film layer by a peptide bond.
Claim 26
Dependent Claim
34 words
A process in accordance with claim 14, wherein said two metal contact-electrodes are connected to a header through gold-plated copper wires with silver epoxy and cured at about 150° C. for about 30 minutes.
Claim 27
Dependent Claim
60 words
A process for measuring antigen-antibody binding reactions comprising: contacting a material comprising a chosen antigen with a biosensor comprising a substrate material, at least one covalently bound antibody corresponding to said chosen antigen immobilized on said substrate material, and impedance detection means for measuring an impedance of said biosensor; and measuring the impedance between two metal contact-electrodes of said biosensor.
Claim 28
Dependent Claim
60 words
A process for measuring antigen-antibody binding reactions comprising: contacting a material comprising a chosen antigen with a biosensor comprising a substrate material, at least one covalently bound antibody corresponding to said chosen antigen immobilized on said substrate material, and impedance detection means for measuring an impedance of said biosensor; and measuring the impedance between two metal contact-electrodes of said biosensor.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-10-22 | 2000-04-22 | 2000-10-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-10-22 | 2004-04-22 | 2004-10-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-10-22 | 2008-04-22 | 2008-10-22 | $7700.00 | Window Closed / Lapsed |