US5053225A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Functional organic thin film chemically bonded to biologically active agent
Assignee: Fujifilm Holdings Corp
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Filing: 1989-03-20
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Granted: 1991-10-01
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Expires: 2009-03-20
Abstract
Abstract A water-insoluble functional organic thin film comprising a binder selected from the group consisting of a high molecular weight organic compound and a lipid, having contained therein a biologically active protein and a polymer containing at least one functional group which forms covalent bonds with said biologically active protein.
Inventors
- 👤 Tsutomu Miyasaka
- 👤 Yukio Maekawa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 03:41
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; C; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/089; C12N11/096
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
79 words
A water-insoluble functional organic thin film comprising a binder selected from the group consisting of a high molecular weight organic compound and a lipid, each having a functional group capable of reacting with an --SO 2 CH═CH 2 group, said binder having contained therein a biologically active protein and a polymer, said polymer comprising at least one of the following monomers: ##STR14## wherein covalent bonds are formed between said polymer and biologically active protein upon hardening of said film.
Claim 2
Dependent Claim
79 words
A water-insoluble functional organic thin film comprising a binder selected from the group consisting of a high molecular weight organic compound and a lipid, each having a functional group capable of reacting with an --SO 2 CH═CH 2 group, said binder having contained therein a biologically active protein and a polymer, said polymer comprising at least one of the following monomers: ##STR14## wherein covalent bonds are formed between said polymer and biologically active protein upon hardening of said film.
Claim 3
Dependent Claim
27 words
A water-insoluble functional organic thin film as claimed in claim 1, wherein said binder is selected from a hydrophilic colloid, a hydrophilic polymer and a mixture thereof.
Claim 4
Dependent Claim
18 words
A water-insoluble functional organic thin film as claimed in claim 2, wherein said binder is gelatin or albumin.
Claim 5
Dependent Claim
33 words
A water-insoluble functional organic thin film as claimed in claim 1, wherein said polymer comprises 1 mol % or more of said at least one monomer based on the amount of said polymer.
Claim 6
Dependent Claim
33 words
A water-insoluble functional organic thin film as claimed in claim 4, wherein said polymer comprises 5 mol % or more of said at least one monomer based on the amount of said polymer.
Claim 7
Dependent Claim
62 words
A water-insoluble functional organic thin film as claimed in claim 1, wherein said polymer is employed in such an amount that the amount of --SO 2 CH═CH 2 groups in the polymer is from 1 to 500 mmol per 100 g of substances having groups capable of reacting with said --SO 2 CH═CH 2 groups contained in said functional organic thin film.
Claim 8
Dependent Claim
60 words
A water-insoluble functional organic thin film as claimed in claim 6, wherein said polymer is employed in such an amount that the amount of said --SO 2 CH═CH 2 groups is from 10 to 100 mmol per 100 g of substances having groups capable of reacting with said --SO 2 CH═CH 2 groups contained in said functional organic thin film.
Claim 9
Dependent Claim
19 words
A water-insoluble functional organic thin film as claimed in claim 1, wherein said biologically active protein is an enzyme.
Claim 10
Dependent Claim
23 words
A water-insoluble functional organic thin film as claimed in claim 1, wherein the molecular weight of said polymer is from 2,000 to 500,000.
Claim 11
Dependent Claim
30 words
A water-insoluble functional organic thin film as claimed in claim 1, wherein the amount ratio of said binder to said biologically active protein is from 1/2 to 100 by weight.
Claim 12
Dependent Claim
36 words
A water-insoluble functional organic thin film as claimed in claim 1, wherein the total amount of said water-insoluble functional organic thin film is from 1 μg to 10 mg per 1 cm 2 of said film.
Claim 13
Dependent Claim
36 words
A water-insoluble functional organic thin film as claimed in claim 11, wherein the total amount of said water-insoluble functional organic thin film is from 10 μg to 1 mg, per 1 cm 2 of the film.
Claim 14
Dependent Claim
29 words
A water-insoluble functional organic thin film as claimed in claim 1, wherein the dry thickness of said water-insoluble functional organic thin film is from 50 Å to 10 μm.
Claim 15
Dependent Claim
28 words
A water-insoluble functional organic thin film as claimed in claim 13, wherein the dry thickness of said water-insoluble functional organic thin film is from 100 to 5,000 Å.
Claim 16
Dependent Claim
23 words
A water-insoluble functional organic thin film as claimed in claim 1, wherein said water-insoluble functional organic thin film is provided on a support.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-09-30 | 1995-04-01 | 1995-10-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-09-30 | 1999-04-01 | 1999-10-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-09-30 | 2003-04-01 | 2003-10-01 | $7700.00 | Window Closed / Lapsed |