US5269903A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Microbioelectrode and method of fabricating the same
Assignee: Individual
•
Filing: 1991-10-22
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Granted: 1993-12-14
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Expires: 2011-10-22
Abstract
Abstract Fine particles 1 of an electrically conductive material such as platinum black are being deposited electrolytically on the end surface of an electrically conductive material (base) such as platinum, with immersion of the conductive material in a solution containing both of a conductive material to produce porous deposition 1, e.g. platinum black, and a biofunctional material (biologically active substance) 2 such as an enzyme, so as to produce a microbioelectrode 7 having fine particle layer 1 and biologically active substance 2 incorporated therein, with significantly high surface area. This microbioelectrode can be compact in size and moreover, since the apparent surface area of the fine particles 1 of the conductive material is significantly large and a considerable amount of the biologically active material 2 can be incorporated and immobilized in the conductive fine particles 1, the intended trace target substance can be measured quickly and with high sensitivity even when it is in a trace amount of sample.
Inventors
- 👤 Yoshito Ikariyama
- 👤 Shigeru Yamuchi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 19:55
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/14; C12Q; C12Q1/00; C12Q1/001; C12Q1/002; C12Q1/003; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
57 words
A biologically active substance immobilized microbioelectrode comprising a fine particle electrically conductive surface layer incorporating an immobilized biologically active substance formed by a single step of: depositing fine particles of a conductive material and said active substance on the surface of an electroconductive material so as to form a porous conductive material incorporating said active substance therewithin.
Claim 2
Dependent Claim
57 words
A biologically active substance immobilized microbioelectrode comprising a fine particle electrically conductive surface layer incorporating an immobilized biologically active substance formed by a single step of: depositing fine particles of a conductive material and said active substance on the surface of an electroconductive material so as to form a porous conductive material incorporating said active substance therewithin.
Claim 3
Dependent Claim
25 words
The microbioelectrode in accordance with claim 1, further comprising the step of: stabilizing the resulting active substance incorporated porous conductive material with a crosslinking agent.
Claim 4
Dependent Claim
30 words
The microbioelectrode in accordance with claim 2, further comprising the step of: forming a polymeric film on the surface of said conductive materials for the stabilization of said active substance.
Claim 5
Dependent Claim
29 words
The microbioelectrode in accordance with claim 1, wherein said porous conductive material is treated by anodic oxidation after the deposition of said porous conductive material incorporating said active substance.
Claim 6
Dependent Claim
20 words
The microbioelectrode in accordance with claim 1, wherein said fine particles are deposited by a solution containing the conductive material.
Claim 7
Dependent Claim
23 words
The microbioelectrode in accordance with claim 1, wherein said fine particles are deposited by a chemical reaction utilized to generate the conductive material.
Claim 8
Dependent Claim
59 words
A microbioelectrode designed to have biologically active substances embodied directly in a transducing electrode, or immobilized in the fine pores or on the surface of a porous electroconductive material which is deposited on the surface of a transducing electrode, whereby both the biochemical reaction and electrochemical reaction occur simultaneously so as to enable direct transduction into an electric signal.
Claim 9
Dependent Claim
59 words
A microbioelectrode designed to have biologically active substances embodied directly in a transducing electrode, or immobilized in the fine pores or on the surface of a porous electroconductive material which is deposited on the surface of a transducing electrode, whereby both the biochemical reaction and electrochemical reaction occur simultaneously so as to enable direct transduction into an electric signal.
Claim 10
Dependent Claim
47 words
A microbioelectrode comprising a fine platinum black electrically conductive surface layer with an immobilized biologically active substance incorporated therein, wherein said microbioelectrode is formed by the single step of depositing particles of said fine platinum black and an active substance on the surface of an electroconductive material.
Claim 11
Dependent Claim
47 words
A microbioelectrode comprising a fine platinum black electrically conductive surface layer with an immobilized biologically active substance incorporated therein, wherein said microbioelectrode is formed by the single step of depositing particles of said fine platinum black and an active substance on the surface of an electroconductive material.
Claim 12
Dependent Claim
25 words
The microbioelectrode in accordance with claim 8, further comprising the step of: stabilizing the resulting active substance incorporated porous conductive material with a crosslinking agent.
Claim 13
Dependent Claim
30 words
The microbioelectrode in accordance with claim 9, further comprising the step of: forming a polymeric film on the surface of said conductive materials for the stabilization of said active substance.
Claim 14
Dependent Claim
29 words
The microbioelectrode in accordance with claim 8, wherein said porous conductive material is treated by anodic oxidation after the deposition of said porous conductive material incorporating said active substance.
Claim 15
Dependent Claim
20 words
The microbioelectrode in accordance with claim 8, wherein said fine particles are deposited by a solution containing the conductive material.
Claim 16
Dependent Claim
23 words
The microbioelectrode in accordance with claim 8, wherein said fine particles are deposited by a chemical reaction utilized to generate the conductive material.
References Cited (Backward Citations)
US4545382A
Cited
US4970145A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-12-13 | 1997-06-14 | 1997-12-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-12-13 | 2001-06-14 | 2001-12-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-12-13 | 2005-06-14 | 2005-12-14 | $7700.00 | Window Closed / Lapsed |