US5543289A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Methods and materials for improved high gradient magnetic separation of biological materials
Assignee: Miltenyi Biotec GmbH
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Filing: 1994-05-31
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Granted: 1996-08-06
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Expires: 2014-05-31
Abstract
Abstract Improvements in the existing procedures and materials for conduct of high gradient magnetic separation (HGMS) are disclosed. Superior superparamagnetic particles, optionally coated with a polysaccharide or other, usually organic, materials can be prepared in uniform compositions with homogeneous magnetizations. The coating can conveniently be conjugated to a specific binding moiety complementary to a biological material whose purification or separation is desired. In addition, plastic coated matrices which form superior magnetic gradient-intensifying supports are disclosed, along with improved methods and apparatus to conduct HGMS.
Inventors
- 👤 Stefan Miltenyi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 08:22
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/54326; G01N33/5434; B; B03; B03C; B03C1/00; B03C1/002; B03C1/005; B03C1/01; B03C1/02; B03C1/025; B03C1/031; B03C1/033; B03C1/0332; B03C1/034; G01N2400/00; G01N2400/10; G01N2400/12; G01N2400/14; G01N2400/22; G01N2446/00; G01N2446/20; G01N2446/30; G01N2446/80; G01N2446/90; Y; Y10; Y10S; Y10S435/00; Y10S435/803
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
63 words
A composition of superparamagnetic colloidal particles, wherein said particles (a) have an organic coating, (b) are conjugated to a specific binding partner, (c) have a lower size limit of 0.04 μm and an upper size limit of 0.1 μm, and (d) when exposed to a magnetic field have magnetizations such that the value of the magnetization in the composition varies less than 10%.
Claim 2
Dependent Claim
63 words
A composition of superparamagnetic colloidal particles, wherein said particles (a) have an organic coating, (b) are conjugated to a specific binding partner, (c) have a lower size limit of 0.04 μm and an upper size limit of 0.1 μm, and (d) when exposed to a magnetic field have magnetizations such that the value of the magnetization in the composition varies less than 10%.
Claim 3
Dependent Claim
30 words
The composition of claim 1 wherein when exposed to a magnetic field said particles have magnetizations such that the value of the magnetization in the composition varies less than 5%.
Claim 4
Dependent Claim
19 words
The particles of claim 1 wherein the particles are bound to a target material by said specific binding partner.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-08-06 | 2000-02-05 | 2000-08-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-08-06 | 2004-02-05 | 2004-08-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-08-06 | 2008-02-05 | 2008-08-06 | $7700.00 | Window Closed / Lapsed |