US4230685A
Expired / Lapsed
US
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Method of magnetic separation of cells and the like, and microspheres for use therein
Assignee: KENDREW BIOSYSTEMS Inc A CORP OF
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Filing: 1979-02-28
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Granted: 1980-10-28
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Expires: 1999-02-28
Abstract
Abstract Magneticaly-responsive microspheres having Protein A associated with the outer surfaces thereof are reacted with antibodies selective to the cells, bacteria, or viruses to be separated from a mixed population to attach the antibodies in oriented relation with their Fab arms extending outwardly, and the microspheres are then used in a magnetic separation procedure. The preferred microspheres are prepared from a mixture of albumin, Protein A, and magnetic particles, the microspheres being prepared so that the Protein A is present in the exterior surfaces for antibody binding.
Inventors
- 👤 Andrew E. Senyei
- 👤 Kenneth J. Widder
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 10:08
CPC Classifications
C; C12; C12N; C12N7/00; A; A61; A61K; A61K35/00; A61K35/12; C12N1/00; C12N1/02; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/54326; G01N33/5434; G01N33/544; C12M; C12M47/00; C12M47/04; C12N2710/00; C12N2710/00011; C12N2710/16011; C12N2710/16051; G01N2333/00; G01N2333/195; G01N2333/305; G01N2333/31; G01N2333/435; G01N2333/76; G01N2446/00; G01N2446/20; G01N2446/30; G01N2446/80; G01N2446/84; Y; Y10; Y10S; Y10S436/00; Y10S436/828
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
155 words
A method for the separation of a select population of cells, bacteria, or viruses from a mixed population thereof, in which microspheres containing magnetic particles are coated with a layer of antibodies which selectively bind to the select population, the coated microspheres are contacted with said mixed population so that said microspheres are bound to the select population, and said bound select population is magentically separated from the rest of said mixed population, wherein the improvement comprises: prior to coating said microspheres with antibodies modifying the surfaces of said microspheres to provide staphylococcal Protein A distributed thereover in adherent relation to said microspheres, then contacting said microspheres with antibodies which bind to Protein A and which also bind selectively to said select population, whereby said antibodies are arranged in oriented attachment on the surfaces of said microspheres with their Fab arms extending outwardly, and thereafter carrying out the rest of the steps of said method.
Claim 2
Independent Claim
155 words
A method for the separation of a select population of cells, bacteria, or viruses from a mixed population thereof, in which microspheres containing magnetic particles are coated with a layer of antibodies which selectively bind to the select population, the coated microspheres are contacted with said mixed population so that said microspheres are bound to the select population, and said bound select population is magentically separated from the rest of said mixed population, wherein the improvement comprises: prior to coating said microspheres with antibodies modifying the surfaces of said microspheres to provide staphylococcal Protein A distributed thereover in adherent relation to said microspheres, then contacting said microspheres with antibodies which bind to Protein A and which also bind selectively to said select population, whereby said antibodies are arranged in oriented attachment on the surfaces of said microspheres with their Fab arms extending outwardly, and thereafter carrying out the rest of the steps of said method.
Claim 3
Dependent Claim
26 words
The method of claim 1 in which said microspheres are formed of a polymer matrix material in admixture with said magnetic particles and said Protein A.
Claim 4
Dependent Claim
31 words
The method of claim 2 in which said matrix material is albumin and said microspheres contain from 2 to 40 parts by weight of Protein A per 100 parts of albumin.
Claim 5
Dependent Claim
19 words
The method of claim 1 in which said Protein A is chemically-bonded to the exterior surfaces of said microspheres.
Claim 6
Dependent Claim
98 words
Microspheres for magnetically sorting of cells, bacteria, or viruses, comprising microspheres formed from an amino acid polymer matrix material in admixture with staphylococcal Protein A, and being of a size from 0.2 to 100 microns diameter, said microspheres containing from 2 to 40 parts by weight of said Protein A per 100 parts of said amino acid polymer and said Protein A being present in the exterior surfaces of said microspheres for antibody binding, said microspheres also containing magnetic particles of a size from 100 to 20,000 Angstroms and in an amount sufficient to make said microspheres magnetically-responsive.
Claim 7
Dependent Claim
98 words
Microspheres for magnetically sorting of cells, bacteria, or viruses, comprising microspheres formed from an amino acid polymer matrix material in admixture with staphylococcal Protein A, and being of a size from 0.2 to 100 microns diameter, said microspheres containing from 2 to 40 parts by weight of said Protein A per 100 parts of said amino acid polymer and said Protein A being present in the exterior surfaces of said microspheres for antibody binding, said microspheres also containing magnetic particles of a size from 100 to 20,000 Angstroms and in an amount sufficient to make said microspheres magnetically-responsive.
Claim 8
Dependent Claim
13 words
The microspheres of claim 5 in which said amino acid polymer is albumin.
Claim 9
Dependent Claim
34 words
The microspheres of claim 5 or claim 6 in which said Protein A is present in an amount of from 10 to 30 parts by weight per 100 parts of said amino acid polymer.
Claim 10
Dependent Claim
92 words
Microspheres for magnetically sorting of cells, bacteria, or viruses, comprising microspheres formed from an aqueous mixture of albumin, staphylococcal Protein A, and magnetic particles, said microspheres having an average diameter of from 0.5 to 2.0 microns, and containing from 2 to 40 parts of said Protein A per 100 parts of said albumin and said Protein A being present in the exterior surfaces of said microspheres for antibody binding, said magnetic particles being of a size not over 300 Angstroms and being present in an amount sufficient to make said microspheres magnetically-responsive.
Claim 11
Dependent Claim
92 words
Microspheres for magnetically sorting of cells, bacteria, or viruses, comprising microspheres formed from an aqueous mixture of albumin, staphylococcal Protein A, and magnetic particles, said microspheres having an average diameter of from 0.5 to 2.0 microns, and containing from 2 to 40 parts of said Protein A per 100 parts of said albumin and said Protein A being present in the exterior surfaces of said microspheres for antibody binding, said magnetic particles being of a size not over 300 Angstroms and being present in an amount sufficient to make said microspheres magnetically-responsive.
Claim 12
Dependent Claim
35 words
The microspheres of claim 8 in which said magnetic particles are Fe 3 O 4 and are present in an amount of from 10 to 150 parts by weight per 100 parts of said albumin.
Claim 13
Dependent Claim
32 words
The microspheres of claim 8 or claim 9 in which said Protein A is present in an amount of from 10 to 35 parts by weight per 100 parts of said albumin.
References Cited (Backward Citations)
US3970518A
Cited
US4018886A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-10-28 | 1984-04-28 | 1984-10-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-10-28 | 1988-04-28 | 1988-10-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-10-28 | 1992-04-28 | 1992-10-28 | $7700.00 | Window Closed / Lapsed |