US4375407A
Expired / Lapsed
US
⏱️ 0.0 years remaining
High gradient magnetic separation device
Assignee: Calspan Corp
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Filing: 1981-06-22
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Granted: 1983-03-01
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Expires: 2001-06-22
Abstract
Abstract An improved high gradient magnetic separation device wherein field gradients are produced by applying a relatively uniform magnetic field to a matrix of filamentary ferromagnetic material contained in a separation chamber, the improvement comprising providing the filamentary material with a coating of a hydrogel polymer. The improved device is particularly useful for the separation of biological entities, such as cells, proteins, organelles, enzymes, and the like for mixtures containing same.
Inventors
- 👤 Paul L. Kronick
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:58
CPC Classifications
B; B03; B03C; B03C1/00; B03C1/02; B03C1/025; B03C1/031; B03C1/033; B03C1/034; G; G01; G01N; G01N33/00; G01N33/48; G01N35/00; G01N35/0098; Y; Y10; Y10S; Y10S435/00; Y10S435/803
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
73 words
A high gradient magnetic separation device comprising a chamber having an inlet and a discharge port through which the materials to be separated are passed, said chamber containing a matrix of ferromagnetic filamentary material, and magnetic means disposed in association with said chamber for applying a magnetic field to said matrix of ferromagnetic filamentary material, wherein the improvement comprises providing said matrix of ferromagnetic filamentary material with a coating of a hydrogel polymer.
Claim 2
Dependent Claim
73 words
A high gradient magnetic separation device comprising a chamber having an inlet and a discharge port through which the materials to be separated are passed, said chamber containing a matrix of ferromagnetic filamentary material, and magnetic means disposed in association with said chamber for applying a magnetic field to said matrix of ferromagnetic filamentary material, wherein the improvement comprises providing said matrix of ferromagnetic filamentary material with a coating of a hydrogel polymer.
Claim 3
Dependent Claim
15 words
The device of claim 1 wherein the hydrogel polymer comprises an acrylic homopolymer or copolymer.
Claim 4
Dependent Claim
27 words
The device of claim 2 wherein the acrylic homopolymer or copolymer comprises monomers selected from the group consisting of acrylamide, hydroxyethethyl methacrylate, N, N'-methylene-bis-acrylamide and methacrylic acid.
Claim 5
Dependent Claim
14 words
The device of claim 3 wherein the hydrogel polymer is a homopolymer of acrylamide.
Claim 6
Independent Claim
113 words
A method for separating a particular biological entity from a mixture of biological entities which comprise contacting the mixture with a conjugate comprising a magnetic particle and a substance having a high degree of affinity for the particular biological entity to be separated, thereby producing a complex of said particular biological entity with said conjugate, and passing the mixture through a high gradient magnetic separation device containing a matrix of ferromagnetic filamentary material having a magnetic field applied thereto, said filamentary material being provided with a hydrogel polymer coating, whereby the complex is attracted to the ferromagnetic filamentary material and held thereon as the remainder of the mixture passes out of the device.
Claim 7
Independent Claim
113 words
A method for separating a particular biological entity from a mixture of biological entities which comprise contacting the mixture with a conjugate comprising a magnetic particle and a substance having a high degree of affinity for the particular biological entity to be separated, thereby producing a complex of said particular biological entity with said conjugate, and passing the mixture through a high gradient magnetic separation device containing a matrix of ferromagnetic filamentary material having a magnetic field applied thereto, said filamentary material being provided with a hydrogel polymer coating, whereby the complex is attracted to the ferromagnetic filamentary material and held thereon as the remainder of the mixture passes out of the device.
Claim 8
Dependent Claim
16 words
The method of claim 5 including the additional step of reducing the magnetic field to zero.
Claim 9
Dependent Claim
21 words
The method of claim 6 including the additional step of washing the complex out of the high gradient magnetic separation device.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-02-28 | 1986-08-30 | 1987-03-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-02-28 | 1990-08-30 | 1991-03-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-02-28 | 1994-08-30 | 1995-03-01 | $7700.00 | Window Closed / Lapsed |