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US5523226A
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US
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Transgenic swine compositions and methods
Assignee: University of Illinois System
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Filing: 1993-05-14
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Granted: 1996-06-04
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Expires: 2013-05-14
Abstract
Abstract Transgenic swine, and compositions and methods for making and using same, are provided. Central to the invention are porcine (Sus scrofa) embryonic stem cell lines and methods for establishing them. Cells of such lines are transformed with exogenous genetic material of interest and then used to provide chimeric swine, which have germ cells comprising the exogenous genetic material. The chimeric swine are bred to provide transgenic swine. Transgenic swine of the invention can be used to provide human proteins or peptide hormones or can be used as xenograft donors.
Inventors
- 👤 Matthew B. Wheeler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 09:12
CPC Classifications
A; A01; A01K; A01K67/00; A01K67/027; A01K67/0275; A01K67/0271; C; C12; C12N; C12N15/00; C12N15/09; C12N15/87; C12N15/873; C12N5/00; C12N5/06; C12N5/0602; C12N5/0603; C12N5/0606; A01K2217/00; A01K2217/05; A01K2227/00; A01K2227/10; A01K2227/101; A01K2227/102; A01K2227/103; A01K2227/108; A01K2267/00; A01K2267/02; C12N2502/00; C12N2502/14; C12N2510/00
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
116 words
A method of obtaining an embryonic stem cell for incorporation into a swine embryo to form a chimeric swine, said method comprising: (a) introducing a cell from a culture made by: (i) culturing dissociated cells from a swine embryo in conditioned stem cell medium in the presence or absence of a feeder layer, and (ii) subculturing the culture until a stable culture with morphological features and growth parameters characteristic of an embryonic stem cell culture is established, into a SCID mouse; (b) allowing a tumor to form in the mouse from the cell; and (c) obtaining an embryonic stem cell from a culture that is shown to be capable of producing a tumor in step b.
Claim 2
Independent Claim
116 words
A method of obtaining an embryonic stem cell for incorporation into a swine embryo to form a chimeric swine, said method comprising: (a) introducing a cell from a culture made by: (i) culturing dissociated cells from a swine embryo in conditioned stem cell medium in the presence or absence of a feeder layer, and (ii) subculturing the culture until a stable culture with morphological features and growth parameters characteristic of an embryonic stem cell culture is established, into a SCID mouse; (b) allowing a tumor to form in the mouse from the cell; and (c) obtaining an embryonic stem cell from a culture that is shown to be capable of producing a tumor in step b.
Claim 3
Dependent Claim
45 words
The method of claim 1, wherein the embryonic stem cell is characterized by an undifferentiated morphology indistinguishable from the morphology of a cell from the culture of step a of claim 1 from which a cell formed a tumor in step b of claim 1.
Claim 4
Independent Claim
85 words
A method for determining the cell types in which a genetic complement is expressed, said method comprising: (a) introducing a swine embryonic stem cell Which comprises the genetic complement into an immunocompromised mouse to produce a tumor; (b) placing the tumor in suitable conditions to allow the tumor to differentiate into a plurality of recognizable cell types and to empress the genetic complement; (c) excising the tumor; and (d) analyzing the differentiated cell types to determine in which cell types the genetic complement is expressed.
Claim 5
Independent Claim
85 words
A method for determining the cell types in which a genetic complement is expressed, said method comprising: (a) introducing a swine embryonic stem cell Which comprises the genetic complement into an immunocompromised mouse to produce a tumor; (b) placing the tumor in suitable conditions to allow the tumor to differentiate into a plurality of recognizable cell types and to empress the genetic complement; (c) excising the tumor; and (d) analyzing the differentiated cell types to determine in which cell types the genetic complement is expressed.
Claim 6
Dependent Claim
27 words
An embryonic stem cell obtained from a culture that is capable of forming a tumor in a SCIDS mouse in accordance with the method of claim 1.
Claim 7
Dependent Claim
11 words
A culture initiated from an embryonic stem cell of claim 4.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-06-04 | 1999-12-04 | 2000-06-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-06-04 | 2003-12-04 | 2004-06-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-06-04 | 2007-12-04 | 2008-06-04 | $7700.00 | Window Closed / Lapsed |