US5175384A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Transgenic mice depleted in mature t-cells and methods for making transgenic mice
Assignee: Genpharm International Inc
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Filing: 1988-12-05
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Granted: 1992-12-29
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Expires: 2008-12-05
Abstract
Abstract Transgenic mice having a phenotype characterized by the substantial absence of mature T lymphocytes otherwise naturally occurring in said mice. The phenotype is conferred in the transgenic mouse by a transgene contained in at least the precursor stem cell of the T lymphocyte which is depleted. The transgene comprises a DNA sequence encoding a T cell receptor polypeptide variant which inhibits maturation of the T lymphocyte.
Inventors
- 👤 Paulus J. A. Krimpenfort
- 👤 Antonius J. M. Berns
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 09:17
CPC Classifications
A; A01; A01K; A01K67/00; A01K67/027; A01K67/0275; A01K67/0276; C; C07; C07K; C07K14/00; C07K14/435; C07K14/705; C07K14/70503; C07K14/7051; C07K16/00; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/8509; A01K2207/00; A01K2207/15; A01K2217/00; A01K2217/05; A01K2217/07; A01K2217/075; A01K2227/00; A01K2227/10; A01K2227/105; A01K2267/00; A01K2267/03; A01K2267/0337; A01K2267/035; A01K2267/0381; C07K2317/00; C07K2317/70; C07K2317/77; C12N2830/00; C12N2830/008
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
66 words
A transgenic mouse having a phenotype characterized by the substantial absence of mature T-cells otherwise naturally occurring in said mouse, said phenotype being conferred by a transgene contained in the somatic and germ cells of said mouse, said transgene comprising the ΔV-TCRβ DNA fragment which encodes a T-cell antigen receptor polypeptide variant, and said polypeptide variant being incapable of mediating T-cell maturation in said transgenic mouse.
Claim 2
Dependent Claim
66 words
A transgenic mouse having a phenotype characterized by the substantial absence of mature T-cells otherwise naturally occurring in said mouse, said phenotype being conferred by a transgene contained in the somatic and germ cells of said mouse, said transgene comprising the ΔV-TCRβ DNA fragment which encodes a T-cell antigen receptor polypeptide variant, and said polypeptide variant being incapable of mediating T-cell maturation in said transgenic mouse.
Claim 3
Independent Claim
92 words
A method for producing a transgenic mouse having a phenotype characterized by the substantial absence of mature T-cells otherwise naturally occurring in said mouse, said method comprising: (a) introducing a transgene into a zygote of a mouse, said transgene comprising the ΔV-TCRβ DNA fragment which encodes a T-cell antigen receptor polypeptide variant, said polypeptide variant being incapable of mediating T-cell maturation in said transgenic mouse, (b) transplanting said zygote into a pseudopregnant mouse. (c) allowing said zygote to develop to term, and (d) identifying at least one transgenic offspring containing said transgene.
Claim 4
Independent Claim
92 words
A method for producing a transgenic mouse having a phenotype characterized by the substantial absence of mature T-cells otherwise naturally occurring in said mouse, said method comprising: (a) introducing a transgene into a zygote of a mouse, said transgene comprising the ΔV-TCRβ DNA fragment which encodes a T-cell antigen receptor polypeptide variant, said polypeptide variant being incapable of mediating T-cell maturation in said transgenic mouse, (b) transplanting said zygote into a pseudopregnant mouse. (c) allowing said zygote to develop to term, and (d) identifying at least one transgenic offspring containing said transgene.
Claim 5
Independent Claim
105 words
A method for producing a transgenic mouse having a phenotype characterized by the substantial absence of mature T-cells otherwise naturally occurring in said mouse, said method comprising: (a) introducing a transgene into an embryo of a mouse, said transgene comprising the ΔV-TCRβ DNA fragment which encodes a T-cell antigen receptor polypeptide variant, said polypeptide variant being incapable of mediating T-cell maturation in said transgenic mouse. (b) transplanting said embryo into a pseudopregnant mouse, (c) allowing said embryo to develop to term, and (d) identifying at least one transgenic offspring containing said transgene, and (e) breeding said offspring to form a transgenic mouse having said phenotype.
Claim 6
Independent Claim
105 words
A method for producing a transgenic mouse having a phenotype characterized by the substantial absence of mature T-cells otherwise naturally occurring in said mouse, said method comprising: (a) introducing a transgene into an embryo of a mouse, said transgene comprising the ΔV-TCRβ DNA fragment which encodes a T-cell antigen receptor polypeptide variant, said polypeptide variant being incapable of mediating T-cell maturation in said transgenic mouse. (b) transplanting said embryo into a pseudopregnant mouse, (c) allowing said embryo to develop to term, and (d) identifying at least one transgenic offspring containing said transgene, and (e) breeding said offspring to form a transgenic mouse having said phenotype.
Claim 7
Dependent Claim
27 words
The method of claim 3 wherein said introducing of said transgene into said embryo is by introducing an embryonic stem cell containing said transgene into said embryo.
Claim 8
Dependent Claim
25 words
The method of claim 3 wherein said introducing of said transgene into said embryo is by infecting said embryo with a retrovirus containing said transgene.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-12-29 | 1996-06-29 | 1996-12-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-12-29 | 2000-06-29 | 2000-12-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-12-29 | 2004-06-29 | 2004-12-29 | $7700.00 | Window Closed / Lapsed |