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US5469854A Expired / Lapsed US ⏱️ 0.0 years remaining

Methods of preparing gas-filled liposomes

Assignee: ImaRx Therapeutics Inc
•
Filing: 1993-06-11
•
Granted: 1995-11-28
•
Expires: 2013-06-11
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Abstract

Abstract Methods of and apparatus for preparing gas-filled liposomes are described. Gas-filled liposomes prepared by these methods are particularly useful, for example, in ultrasonic imaging applications and in therapeutic drug delivery systems.

Inventors

  • 👤 Evan C. Unger
  • 👤 Thomas A. Fritz
  • 👤 Terry Matsunaga
  • 👤 VaradaRajan Ramaswami
  • 👤 David Yellowhair
  • 👤 Guanli Wu

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-23 02:11
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/10; A61K9/127; A61K9/1277; A61K9/1278; A61K41/00; A61K41/0028; A61K41/0052; A61K47/00; A61K47/50; A61K47/69; A61K47/6921; A61K47/6925; A61K49/00; A61K49/06; A61K49/18; A61K49/1806; A61K49/1815; A61K49/22; A61K49/222; A61K49/223; A61K49/227; A61M; A61M5/00; A61M5/178; A61M5/31; A61M5/3145; Y; Y10; Y10S; Y10S977/00; Y10S977/70; Y10S977/788; Y10S977/795; Y10S977/797; Y10S977/798; Y10S977/799; Y10S977/801; Y10S977/902; Y10S977/904; Y10S977/905; Y10S977/906; Y10S977/907; Y10S977/927; Y10S977/929; Y10S977/962

Claims Analysis

Total Claims: 20
Claim 1
Independent Claim 38 words
A method for preparing gas-filled lipid microspheres comprising shaking an aqueous solution comprising a lipid in the presence of a gas at a temperature below the gel state to liquid crystalline state phase transition temperature of the lipid.
Claim 2
Independent Claim 38 words
A method for preparing gas-filled lipid microspheres comprising shaking an aqueous solution comprising a lipid in the presence of a gas at a temperature below the gel state to liquid crystalline state phase transition temperature of the lipid.
Claim 3
Dependent Claim 12 words
A method as in claim 1 wherein the shaking step comprises vortexing.
Claim 4
Dependent Claim 16 words
A method as in claim 1 further comprising filtering and heat sterilizing said aqueous lipid solution.
Claim 5
Dependent Claim 21 words
A method as in claim 1 further comprising extruding the microspheres through at least one filter of a selected pore size.
Claim 6
Dependent Claim 16 words
A method as in claim 4 wherein the pore size is about 10 μm or smaller.
Claim 7
Dependent Claim 20 words
A method as in claim 1 further comprising hydrating a dried lipid to form an aqueous solution comprising a lipid.
Claim 8
Dependent Claim 30 words
The method of claim 1 wherein said microsphere comprises a lipid selected from the group consisting of dipalmitoylphosphatidylcholine, dipalmitoylphosphatidylethanolamine, and a phosphatidic acid, and said microsphere further comprising polyethylene glycol.
Claim 9
Dependent Claim 14 words
The method of claim 1 wherein said lipid comprises at least one dipalmitoyl lipid.
Claim 10
Dependent Claim 13 words
The method of claim 1 wherein said lipid comprises dipalmitoylphosphatidylethanolamine and polyethylene glycol.
Claim 11
Dependent Claim 16 words
The method of claim 1 wherein said lipid comprises dipalmitoylphosphatidylcholine, phosphatidic acid, dipalmitoylphosphatidylethanolamine, and polyethylene glycol.
Claim 12
Dependent Claim 24 words
The method of claim 1 wherein said microsphere comprises a gas selected from the group consisting of a fluorine gas and a nitrogen gas.
Claim 13
Dependent Claim 16 words
The method of claim 1 wherein said microsphere comprises a gas which is a fluorine gas.
Claim 14
Dependent Claim 14 words
The method of claim 1 wherein said microsphere comprises a gas which is nitrogen.
Claim 15
Dependent Claim 46 words
The method of claim 1 wherein said microsphere comprises a gas selected from the group consisting of a fluorine gas and a nitrogen gas and a lipid selected from the group consisting of dipalmitoylphosphatidylcholine, dipalmitoylphosphatidylethanolamine, and a phosphatidic acid, and said microsphere further comprising polyethylene glycol.
Claim 16
Dependent Claim 35 words
The method of claim 1 wherein said microsphere comprises a gas selected from the group consisting of a fluorine gas and a nitrogen gas and a lipid comprising dipalmitoylphosphatidylcholine, phosphatidic acid, dipalmitoylphosphatidylethanolamine, and polyethylene glycol.
Claim 17
Dependent Claim 24 words
The method of claim 1 wherein said microsphere comprises a gas which is a fluorine gas and a lipid comprising dipalmitoylphosphatidylethanolamine and polyethylene glycol.
Claim 18
Dependent Claim 11 words
The method of claim 1 wherein said microsphere comprises 90% gas.
Claim 19
Dependent Claim 29 words
The method of claim 1 wherein said microsphere comprises a gas which is a fluorine gas and a lipid comprising dipalmitoylphosphatidylethanolamine and polyethylene glycol, said microsphere comprising 90% gas.
Claim 20
Dependent Claim 12 words
The method of claim 1 performed at a pressure above ambient pressure.

References Cited (Backward Citations)

US3532500A Cited
US3873564A Cited
US4089801A Cited
US4162282A Cited
US4229360A Cited
US4224179A Cited
US4229360B1 Cited
US4192859A Cited
US4310506A Cited
WO1980002365A1 Cited
US4276885A Cited
US4310505A Cited
US4344929A Cited
US4315514A Cited
US4331654A Cited
US4442843A Cited
WO1982001642A1 Cited
US4681119A Cited
US4657756A Cited
US4533254A Cited
US4586512A Cited
US4426330A Cited
US4534899A Cited
US4569836A Cited
US4466442A Cited
EP0107559A1 Cited
US4646756A Cited
US4603044A Cited
US4718433A Cited
US4572203A Cited
US4544545A Cited
US4900540A Cited
WO1985001161A1 Cited
US4675310A Cited
US4728575A Cited
US4658828A Cited
WO1986000238A1 Cited
US5008050A Cited
US4620546A Cited
WO1986001103A1 Cited
US4921706A Cited
US4830858A Cited
US4689986A Cited
US4895719A Cited
EP0216730A2 Cited
US4684479A Cited
US4938947A Cited
US4774958A Cited
US4987154A Cited
US4927623A Cited
US4865836A Cited
US4737323A Cited
US4877561A Cited
GB2193095A Cited
US4728578A Cited
US4776991A Cited
JPS6360943A Cited
US4781871A Cited
US4790891A Cited
US5195520A Cited
US5049388A Cited
US4863717A Cited
EP0272091A2 Cited
US5219538A Cited
US5000960A Cited
EP0314764B1 Cited
WO1989005040A1 Cited
US4844882A Cited
EP0324938A1 Cited
US4898734A Cited
EP0338971A1 Cited
US5171755A Cited
US4893624A Cited
US5045304A Cited
US4957656A Cited
US5316771A Cited
EP0361894A2 Cited
US5198225A Cited
WO1990004943A1 Cited
WO1991000086A1 Cited
US5194266A Cited
US5013556A Cited
US5213804A Cited
US5088499A Cited
US5271928A Cited
WO1991015244A2 Cited
EP0458745A1 Cited
US5215680A Cited
US5310540A Cited
WO1992010166A1 Cited
US5144703A Cited
WO1992017212A1 Cited
WO1993005819A1 Cited
WO1993006869A1 Cited
WO1993013809A1 Cited
EP0554213A1 Cited
WO1993020802A1 Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1998-11-27 1999-05-29 1999-11-28 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 2002-11-27 2003-05-29 2003-11-28 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2006-11-27 2007-05-29 2007-11-28 $7700.00 Window Closed / Lapsed

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