US5045304A
Expired / Lapsed
US
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Contras agent having an imaging agent coupled to viable granulocytes for use in magnetic resonance imaging of abscess and a method of preparing and using same
Assignee: Wayne State University
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Filing: 1988-08-31
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Granted: 1991-09-03
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Expires: 2008-08-31
Abstract
Abstract A method and contrast agent for magnetic resonance imaging (MRI) of abscess. Granulocytes are separated from whole blood and incubated with an imaging vehicle such as magnetite. The granulocytes encapsulate the imaging vehicle and are suspended in an intravascularly administrable carrier to form the contrast agent. The contrast agent is highly specific for MRI of abscess.
Inventors
- 👤 David R. Schneider
- 👤 Kostaki G. Bis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 04:03
CPC Classifications
G; G01; G01R; G01R33/00; G01R33/20; G01R33/44; G01R33/48; G01R33/54; G01R33/56; G01R33/5601; A; A61; A61K; A61K49/00; A61K49/06; Y; Y10; Y10T; Y10T436/00; Y10T436/24
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
112 words
A method of preparing and using contrast agent in magnetic resonance imaging abscesses in a patient comprising the steps of: mixing a specific number of viable granulocytes with an aqueous solution of a metallic imaging agent in which said imaging agent binds to and is subsequently incorporated into the granulocytes incubating said aqueous solution to encapsulate said imaging agent in said granulocytes to form labeled granulocytes; separating said labeled granulocytes from said solution; suspending said labeled granulocytes in an intravascularly administerable carrier to form said contrast agent; administering a diagnostically effective amount of said contrast agent by intravenous infusion to said patient; and subjecting said patient to examination by magnetic resonance imaging.
Claim 2
Independent Claim
112 words
A method of preparing and using contrast agent in magnetic resonance imaging abscesses in a patient comprising the steps of: mixing a specific number of viable granulocytes with an aqueous solution of a metallic imaging agent in which said imaging agent binds to and is subsequently incorporated into the granulocytes incubating said aqueous solution to encapsulate said imaging agent in said granulocytes to form labeled granulocytes; separating said labeled granulocytes from said solution; suspending said labeled granulocytes in an intravascularly administerable carrier to form said contrast agent; administering a diagnostically effective amount of said contrast agent by intravenous infusion to said patient; and subjecting said patient to examination by magnetic resonance imaging.
Claim 3
Dependent Claim
23 words
The method of claim 1 wherein said separating step further comprises attracting said labelled granulocytes from said aqueous solution with a pole magnet.
Claim 4
Dependent Claim
22 words
The method of claim 1 further comprising after the separating step, the step of washing the labelled granulocytes with a physiologic buffer.
Claim 5
Dependent Claim
18 words
The method of claim 1 in which said imaging agent is a ferromagnetic metal or non-toxic salt thereof.
Claim 6
Dependent Claim
18 words
The method of claim 1 in which said imaging agent is a paramagnetic metal or non-toxic salt thereof.
Claim 7
Dependent Claim
18 words
The method of claim 1 in which said imaging agent is a superparamagnetic metal or non-toxic salt thereof.
Claim 8
Dependent Claim
17 words
The method of claim 1 in which said imaging agent is magnetite; Fe 2 O 3 .FeO.
Claim 9
Dependent Claim
19 words
The method of claim 1 in which the incubating step further comprises bubbling O 2 through said aqueous solution.
Claim 10
Dependent Claim
31 words
The method of claim 1 in which the imaging agent has the formula F 2 O 3 .XO, where X is one of the group consisting of para-magnetic and ferro-magnetic material.
Claim 11
Dependent Claim
59 words
A contrast reagent for MRI of abscesses, said reagent comprising: a suspension of labelled granulocytes in an intravascularly adminstrable carrier, said granulocytes binding to and subsequently incorporating within a diagnostically effective amount of an imaging agent, said agent having the formula Fe 2 O 3 .XO where X is one of the group consisting of para-magnetic and ferro-magnetic materials.
Claim 12
Dependent Claim
59 words
A contrast reagent for MRI of abscesses, said reagent comprising: a suspension of labelled granulocytes in an intravascularly adminstrable carrier, said granulocytes binding to and subsequently incorporating within a diagnostically effective amount of an imaging agent, said agent having the formula Fe 2 O 3 .XO where X is one of the group consisting of para-magnetic and ferro-magnetic materials.
Claim 13
Dependent Claim
17 words
The contrast agent of claim 10 wherein said imaging agent is magnetite; Fe 2 O 3 .FeO.
Claim 14
Dependent Claim
13 words
The contrast agent of claim 10 wherein said intravascularly adminsterable carrier is plasma.
References Cited (Backward Citations)
US4269821A
Cited
US4452773A
Cited
US4497791A
Cited
US4672040A
Cited
US4554088A
Cited
US4628037A
Cited
US4615879A
Cited
US4755375A
Cited
US4714607A
Cited
US4687658A
Cited
US4612185A
Cited
US4639364A
Cited
US4637929A
Cited
US4675173A
Cited
US4789541A
Cited
US4669481A
Cited
US4729892A
Cited
US4770183A
Cited
US4935223A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-09-02 | 1995-03-04 | 1995-09-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-09-02 | 1999-03-04 | 1999-09-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-09-02 | 2003-03-04 | 2003-09-03 | $7700.00 | Window Closed / Lapsed |